LOCUS       sequence-491803-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with HBB IVS2 intron within BFP coding 
            sequence and blasticidin resistance gene. Contains Cp36 
            recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA074 Cas9-BFP/HBB_IVS2/BFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgagtctat gggacgcttg atgttttctt tccccttctt ttctatggtt aagttcatgt
       61 cataggaagg ggataagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca
      121 tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt
      181 ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact
      241 taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta
      301 aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt
      361 tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta
      421 tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag
      481 ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt
      541 atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat
      601 catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc
      661 aatatctctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata
      721 ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg
      781 ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt
      841 cctcccacag
//
