LOCUS       sequence-49181-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AKR7A2
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt cccggccacc gccaccgcgg gtcgcctcgg tgctgggcac catggagatg
      121 gggcgccgca tggacgcgcc cgccagcgcc gcggccgtgc gcgcctttct ggagcgcggc
      181 cacaccgaac tggacacggc cttcatgtac agcgacggcc agtccgagac catcctgggc
      241 ggcctggggc tcgggctggg cggtggcgac tgcagagtga aaattgccac caaggccaac
      301 ccttgggatg gaaaatcact aaagcctgac agtgtccggt cccagctgga gacgtcattg
      361 aagaggctgc agtgtcccca agtggacctc ttctacctac acgcacctga ccacggcacc
      421 ccggtggaag agacgctgca tgcctgccag cggctgcacc aggagggcaa gttcgtggag
      481 cttggcctct ccaactatgc tagctgggaa gtggccgaga tctgtaccct ctgcaagagc
      541 aatggctgga tcctgcccac tgtgtaccag ggcatgtaca acgccaccac ccggcaggtg
      601 gaaacggagc tcttcccctg cctcaggcac tttggactga ggttctatgc ctacaaccct
      661 ctggctgggg gcctgctgac tggcaagtac aagtatgagg acaaggacgg gaaacagcct
      721 gtgggccgct tctttgggaa tagctgggct gagacctaca ggaatcgctt ctggaaggag
      781 caccacttcg aggccattgc gttggtggag aaggccctgc aggccgcata tggcgccagc
      841 gcccccagtg tgacctcggc tgccctccgg tggatgtacc accactcaca gctgcagggt
      901 gcccacgggg acgcggtcat cctgggcatg tccagcctgg agcagctgga gcagaacttg
      961 gcagcaacag aggaagggcc cctggagccg gctgtcgtgg atgcctttaa tcaagcctgg
     1021 catttggttg ctcacgaatg tcccaactac ttccgctaac agtaaaggtg gatac
//
