LOCUS       sequence-491812-        2323 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with EIF4A3 IVS2 intron in 5' UTR and 
            blasticidin resistance gene. Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA090 EIF4A3_IVS2-Cas9-BFP
FEATURES             Location/Qualifiers
     source          1..2323
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtaagttgtc ctggggtgcg gcgcagggct ggatgtaaat ttgggcagcg cagtgcagtt
       61 agatgctctg ggatgactgt ttccctcagc ttgtacacgc tcagttccaa cactttccct
      121 gactttagcc acagggttta atactttttt aaatttgtgt tctctactgt ggaaagcttt
      181 cattttcttt ttgagatgga gtctcgctct gttgcccagg ctggagtgca gtggtgccat
      241 ctcggctcac tgcaaccttt gcctctcagt ttcaagctat tctcctgcct caccctccca
      301 agtagctggg attacaggca tgcaccacca tgcccgacta attttcatat ttttagtaga
      361 gacgggggtt tcaccatgtt ggtcaggctg gtctcaaact cctgacctca ggtgatccac
      421 cctccttggc ctcctaaagt gctgggattt caggcatgag ttactgcgcc cggccaaggc
      481 tttcattttc ttagccagct ttgcttagtg gtttgctggc atgtggaatt gtatataaca
      541 caatttcgga aaaacagact gtgcttttcc tctgctctca caccaccaca acagtcatca
      601 gcacagaaga agacttctgt gagcagaggt gtggggcttt tccagctggg tgtcctccag
      661 ttcaactcac accacctgcc tggagatagg ccatatcctg caggttgacg gctcagtccc
      721 catgactacc ctcttttcag acaccagtta taagttcgga tctctagaac ttctgaccaa
      781 gcagcttcaa gttgggttcc caggatcccc tctttaggtt ggattaattt gctggaacag
      841 cagctcaaag aactcaggga aacacattta gtggtttatt ataaagggta ctgcagagga
      901 tacagatatg gggggagggg tgcagcctcc aaggggcggg tggtcacctc ccggaacctc
      961 cttgtgttcc actgtctgtg ttcagccatc tagaagctct tcgaacccag tcctcttggg
     1021 tttttatgga aaattccatg aggtcagcat tctttccccc ataagggaat agggcaggag
     1081 cttttctggg ataataccat aatgcttttt aaaaaaaaat ttgaaattta aattttttac
     1141 atttttcgag acagggtctt actctgtctc ccagactgga gtgcagtcgt gtgattacag
     1201 ctcatagcag cctcaagcga tcctcccacc tcaccctccc aagtagctgg gactacaggc
     1261 acacatcacc atgcccaatt aatttttgta ttttctgtag agacagggtt ttgttgtgtt
     1321 gcccaggctg ctctcaaact cctggcaatc tgtctgcctt ggcttcccaa agccttggga
     1381 ttacaggcgt gagccactgt gcccggccta aacatttttt ttgttaagat atggggcctc
     1441 acttcgtcac ccaggcggga atgcagtggt gcagtcatag ctcactgcaa cctctaactt
     1501 cgaggattaa ggaatcgtcc cgcctcaacc tcccaagtag ctgggactgc cagaatgtgc
     1561 caccgttcct ggttagtttt ttgttttgtt tagaaatggg gtctcttgct gtattgccca
     1621 ggctggtctc gaattcctgg cttcaattaa tgctgcctct gcgtcccaga gtgttgggaa
     1681 tacaggcctg agccaccacg ccgtgacatg agggtcttta tgactcacaa tctgaaaggc
     1741 agggaagatt agagccctgc tgtggagcag gtgaaaggag agcagaaggt cagagagatt
     1801 gtttcctgag gcctaaccca tcaatttaat aagactgtaa taagggctat gggagctgtg
     1861 agccaggaac tatggatgaa aacttgtata tagccatatc tcctagcacc acaaatcctc
     1921 ttttaatagc tttattcctc gtttgcctaa tttcaaaaaa gactcattta cacaaaaatt
     1981 tctttggaag agagagttag tccaaaatcc tagaaggaaa agagttgtat ttagataaag
     2041 gggagtcctc ctttttctga cacctatgaa acacctcttg aagttagaga gtctgtggct
     2101 ccattgcccc cgtgcagaag ccatgtctac tctctttctt catggacact taacaagctg
     2161 tgacactggg cccgagtcct gcctcctcgc agttaagatg ttattaggta cgggggcttg
     2221 ctggaagaag gtacctaagt gcctctggag cccaggcctt ggagtgcagc tgtggcgtgg
     2281 cctgtcctcc ctctaatccc actgcttttg tttccttgaa tag
//
