LOCUS       sequence-491814-        2252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with PSMD14 IVS2 intron in 5' UTR and 
            blasticidin resistance gene. Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA091 PSMD14_IVS2-Cas9-BFP
FEATURES             Location/Qualifiers
     source          1..2252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtaaggttta aaaatgtatt ttacttttaa ttattatttt acggatggat agagagatag
       61 tatgaaaatg tattattttt aacagatgtc aaattatgaa tgtgattgat aaccagattt
      121 tgaaaagtgg tgtgatttag aatatgtatc aatatacttc attaccaaat gttagtattt
      181 ttattaaacc tggttatctc agcttaaagt tttaatttgt taaatatgtt tcctctttgc
      241 tgtgatatct aattttgcta cagaaacaaa tttgacctgc cagattttct ttctctctgc
      301 ttcttgcatc actcatatat attcacagca tctggagtct gtgtaaagtc atcagaaaca
      361 acacagaggg atagataaac tgaacacagc tgggaatcag ttttggccta gaatatatat
      421 attctaatgt atactgtatg ttgtttgttt tttctgttgg ttctttaagt ttgtaaggtt
      481 gaaatggtcc aaaagaaatt tttttcttct ttggtaatat attctagaga attgctttaa
      541 tattataaaa tatacaggat tccccccgcc ccgtttttag cctttaaaaa gtgctgtgtg
      601 ttgaatgcca tatttgattt gaggaatttc aatgagtatt tgatggagat tttatttgta
      661 aatgttgcta ttaggaggat gagttaagat cgtgaagtgg aaattttgtc tgtattacca
      721 cgttttctta tatgcctttt cattttaaaa aaggaatttt taaaattgtg agtcttataa
      781 acatgttaca tgaaacaagc cagatacgaa aaatatatga ttccgtttat gtaaaattca
      841 aaagcagcca aactaatctg tgctgttgaa agttgaattt tacctttgca gggtggaata
      901 gactggaagc ggggcttggg tggcgtttct gaggtgccaa taatgttctg tttcttgaac
      961 taggtgatgg ttacatggat gtgttcactt ttgaagattc catggccata tcgtttgttt
     1021 tcttttctgt atgtgcactt gagtgaagtt cttttctgta tgtggacttg aataaagttt
     1081 gcgatattaa cattgttaaa ttgttaccag gattcaacaa aggtgattgg tgggggataa
     1141 tatataagta cattattgag ttttagttta tatagcaatt tgaactcgta aaacaacatg
     1201 gacgaaggat gaaatgaatc ttagcattta atttagaata taatttgtaa taataccatt
     1261 ttcttaagca tacacttagg aagaagagga atttcattta ggacatagcg attttcaagt
     1321 agaaaaaatt gctttgttgt catttttaaa ttacttagtt gtactgttat taacttttaa
     1381 aatttgatta cttctttttg ttatttaagg ccatataagt acacttgata tttcttaact
     1441 tcttctgtga ggttttcaag cttgtaagag tatcttctgt agttactcta aacgttaaaa
     1501 ctaaatcaga ttgatatagt aaatccactt tgtattggtt aactctgaag ttataattga
     1561 gaagcacagt tgaatttcct tagtgaaaaa ggtgtatata tctctacaac atctgaaaca
     1621 acacaaaata tctgtgtagg ttctagaaca ctatcttaat atgtttaatg tttatgctat
     1681 agtatttctt ttagtataat gagttcagtt tttaaaagcc tttgatgcta attcagagat
     1741 ccataatctg ctcacaatgt ctttcacatg tagttttgat ggtaactgac aagctgtaag
     1801 ttaattagca cagaacttac taatgaggat gttaaaattt aatttaaaag tgaggatttt
     1861 tatttgtaca ctctggacca attgaaggaa attcaatcat acatatgata agcatttcag
     1921 gcactttttc tacattcagg tactttatta gataacaata agaaaatata attaacttaa
     1981 tgatagcaaa gttttgaaag tatgctaaga ttgcttgtct ttaaaactgg ggataaccca
     2041 aaaccaaagc agtaaaataa ggtctgttaa ttagcatgga agacagcttc ttgaattaat
     2101 atgtggtgtt aattaacagg aagtctgatg ttgtgttgta attaccacca atatttttga
     2161 catactgagt gactgaaggt gaattatata gatagcaatt gaataaacat ttttgtgctt
     2221 aggaacgttt tttctttgtt tctgttttct ag
//
