LOCUS       sequence-491818-        1188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with PFDN2 IVS3 intron in 5' UTR and 
            blasticidin resistance gene. Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA093 PFDN2_IVS3-Cas9-BFP
FEATURES             Location/Qualifiers
     source          1..1188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgagcaaga atcattgaat aggatcgggt acagtggctg taaacccagc actttgggag
       61 gccaaggcag gtggactctt tgagtccggg agttcgagac cagcctgggc aacatggtga
      121 aaccatatca ctacaaaaaa tacaaaagat tagctgggta tggtggtatg tgcctgtagt
      181 cccagctact cacctgagcc taaaatgtcg aggcttcagt gagctgtgat cacctcactg
      241 cactccagcc tgggtgacag agtgagaccc tgtctcagat aaaaggaggg cagcataaaa
      301 tagcctatgc tcattatgct atgctaagtg caactcttag acaagaggaa ttgctgactg
      361 ctgtgggctc tgtgacttgg ctgaattggg gtgggaggat tggggttaga gtaatgtttc
      421 tcatagtgtg tgtttcacga atacatgaat caaaatcacc tgaaggacta atttataaat
      481 gcagcttccc agacccactg gattagaacc tttgggagtg ccatgaagtg acattttaaa
      541 aataagctag gcaggttgca gtgactcaca cctgtaatcc cagcactttg ggaggccgag
      601 gcgggcggat cacgaggtca ggaggtcaag accatcctgg ctagcatggt gaaaccccat
      661 ctctactaag aatacgaaaa attggctggg cgttgtggtg ggcgcctgtg gtcctagcta
      721 ctcgggaggg caaggcagga gaatggcgtg aacctgcgag gcggagcttg cagtgagctg
      781 agattgcacc actgcactcc agcctgggcg acacagcgat gctgcaactc aaaaaaaaaa
      841 tattaaaaat aaaaaatgaa aaaataaaaa taagctcatg cctgtattcc tagcgctttg
      901 ggagaccgag gcaggaggac tgcttgaggc agaagtttga gaccagcctg ggtaacatag
      961 taggaacttg tctctgtaaa aaataaaaat aagcagccca ggtgattcta agtacattaa
     1021 agtgtataaa ccactgtgtt caagaaagga ataattttaa tgtctgggct cctctacttg
     1081 gttaaaggta aggttaaatg tgttaacact tcctccaacc atagtccatt ccaggtgggg
     1141 gaaaggagtc ctgaattcct tatataatcc ctgtcacttg tcctctag
//
