LOCUS       sequence-491820-        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with UBA1 IVS20 intron in 5' UTR and 
            blasticidin resistance gene. Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA094 UBA1_IVS20-Cas9-BFP
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgagggtgt ttggccagtt ggccaggagt caccccacat gtccccggcc tagtccagcc
       61 tccccaccag tcctctaccc ctggttctgc tctgctctgt gaccccaggc ctgaggttta
      121 cccacacctt cctttgagcc tccctttgac atgaagaggg taggttgggg caaattatct
      181 ccctccctcc tcctccttcc cactatctgg ccctcaggag aacttgctgt tttactcttg
      241 ctctgtgtat ttcccttagt tttgttcttt cttctgtttc ttcttgatct gttcatctag
      301 gaggtatgat ctctaccagc agtctctctc ccttgatact ttcctgcctt gtctccttcc
      361 agatccgccc ccatctgtac atctacctgc atgtttctct gtgcatcttc accctccata
      421 gtttggggct gcggtttact gacaccccca ccccacccca tctgcatatt ttttcaccac
      481 ccctcccttc tgtatatgat gcttctgtag ctctgtaacg ccccctacat ttaccttcct
      541 tatatctccc ccgtcttcct ctccatagat ctcctcccat ttccccttcc atggtcccca
      601 tcttccttct gaaatgtcta ctccttcatg ttcctttatg tatgtcttcc aatctttcct
      661 tccatagctc tcatcacctt catatatttc ttccatcttt ctcctcccac ctgcctcgcc
      721 ctctgtatat acccccactc tccccctttt atatcttctc catctccccc catatctttc
      781 ctctatgtcc acatctgtgt attcccccca acttcccctc catatatctt ttttactccc
      841 cttttcctcc ctgtatcctc tgtgttcccc ccatcttgct ctacatcatt cttcccaaga
      901 tctttacgtc tcccatcttg atctctccat ctccactttc tcctaacatt ttcatttccg
      961 ttccttagtg tctctagaga gatcattctt gatagcctca gctctttctc tgtgtttttc
     1021 aggtttgtat tctgctctgc tctacctctc ctccttgccc cttttctctc ccaggatgtc
     1081 tctcctttcc aaatcctttt tgtacctgaa taccttttgc cccaccctgg gctctcattt
     1141 ccatctcaga ccttagcctg ggatctaaag ggctgacagt gtccctttct tcatgcag
//
