LOCUS       sequence-491822-         447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with EEF2 IVS9 intron in 5' UTR and 
            blasticidin resistance gene. Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA095 EEF2_IVS9-Cas9-BFP
FEATURES             Location/Qualifiers
     source          1..447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgagcagct gggtgatgtg caggcccctt ggcactgttg cgggccctgc cctgctccaa
       61 gtcttgggct cctcagtcgg gagctagaag taaggagcat agaaggaata tagggtgata
      121 cttgtgccag ccacaacttt tgttcttagc ccagtgaggg catttctgga gaagctccaa
      181 gccccagggc acagcacacc cctgctcatc tgtgtccctg ggcacaggtg ggtggcggcc
      241 gtcgacccat acccagggag gagggcagtg attctgaaga ggatgtttcc tgacagcccc
      301 gacgcagcag tcagggcctt gtggaagcca ccagtctgcc ctgggacctg gcaggagggc
      361 caccaactcc gggagggacc tgccctggcc ctccagggtc cagcacaacc atccctgcgg
      421 cctgctgccg ccttctgctt cttccag
//
