LOCUS       sequence-491826-         408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with HNRNPK IVS3 intron in 5' UTR and 
            blasticidin resistance gene. Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA097 HNRNPK_IVS3-Cas9-BFP
FEATURES             Location/Qualifiers
     source          1..408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgagaagaa attttagttt taacagtatt tttttatcct gttaataaat tgcctttttc
       61 tgttaattat tgccttagga gtgtgtggtg aatttttggt attactgttt atgtaattct
      121 gcgattatca gtagttacat cttagctttt atttatgaag tagaagtaac tgatttgcat
      181 ataaagaatt tttcttgcat tttgggattt caaatccttt gaagtatttt gtggcagtat
      241 tttgtgtttg gtgcatttta ctagatggaa gcataacgtt caagtctgta gcaatgcctt
      301 tggtcttttc aaaacatgcc taagattagc aacaagtctc ttgaaacaga atggagttac
      361 tttggagact tcgctgcttt ctttgaaatg tgaactgtta atttttag
//
