LOCUS       sequence-49183-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PIM1
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc tcttgtccaa aatcaactcg cttgcccacc tgcgcgccgc gccctgcaac
      121 gacctgcacg ccaccaagct ggcgcccggc aaggagaagg agcccctgga gtcgcagtac
      181 caggtgggcc cgctactggg cagcggcggc ttcggctcgg tctactcagg catccgcgtc
      241 tccgacaact tgccggtggc catcaaacac gtggagaagg accggatttc cgactgggga
      301 gagctgccta atggcactcg agtgcccatg gaagtggtcc tgctgaagaa ggtgagctcg
      361 ggtttctccg gcgtcattag gctcctggac tggttcgaga ggcccgacag tttcgtcctg
      421 atcctggaga ggcccgagcc ggtgcaagat ctcttcgact tcatcacgga aaggggagcc
      481 ctgcaagagg agctggcccg cagcttcttc tggcaggtgc tggaggccgt gcggcactgc
      541 cacaactgcg gggtgctcca ccgcgacatc aaggacgaaa acatccttat cgacctcaat
      601 cgcggcgagc tcaagctcat cgacttcggg tcgggggcgc tgctcaagga caccgtctac
      661 acggacttcg atgggacccg agtgtatagc cctccagagt ggatccgcta ccatcgctac
      721 catggcaggt cggcggcagt ctggtccctg gggatcctgc tgtatgatat ggtgtgtgga
      781 gatattcctt tcgagcatga cgaa
//
