LOCUS       sequence-491830-         116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with PGK1 IVS3 intron in 5' UTR and 
            blasticidin resistance gene. Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 116)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA099 PGK1_IVS3-Cas9-BFP
FEATURES             Location/Qualifiers
     source          1..116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtaagtgcca ggctctggtg ctggtagact ttgtggcggg ggaagttgtc aagcacgttg
       61 ttactggttt ttaactttca tactgctcaa gtgtcttcat ctcttcctct tctcag
//
