LOCUS       sequence-491840-         231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and 
            blasticidin resistance gene. Flanked by cHS4 core insulators. 
            Contains Cp36 recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pSA144 HBB_IVS2-Cas9-BFP with core cHS4 insula...
FEATURES             Location/Qualifiers
     source          1..231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg
       61 aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg
      121 gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc
      181 gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc t
//
