LOCUS       sequence-49185-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK1
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      302..311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg aagagaagct gaagaaaacc aatatcatct ttgtggtggg tgggcctggc
      121 tcagggaagg gcacccagtg tgagaagatc gtgcagaagt atggctacac ccacctctcc
      181 accggggacc tcctgcggtc cgaggtcagc tcaggctcgg ccaggggcaa gaagctgtcg
      241 gaaatcatgg agaaggggca gctggttcca ctggagacag tgttggacat gctccgggat
      301 gccatggtgg ccaaagtcaa tacttccaaa ggcttcctga ttgatggcta cccgcgggag
      361 gtgcagcaag gagaagagtt tgagcgacgg attggacagc ccacactgct gctgtatgtg
      421 gacgcaggcc ctgagaccat gacccagcgg ctcttgaaac gtggagagac cagcgggcgt
      481 gtggacgaca atgaggagac catcaaaaag cggctggaga cctattacaa ggccacagaa
      541 cccgtcatcg ccttctatga gaaacgtggc attgtgcgca aggtcaacgc tgagggctcc
      601 gtggacagtg tcttctccca ggtctgcacc cacctggacg ccctattgaa ttgacagtaa
      661 aggtggatac
//
