LOCUS       sequence-491851-         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with
            blasticidin resistance gene. Contains minimal A2UCOE and Cp36 
            recombination site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, 
            Garcia CA, Nguyen T, Venida A, Seczynska M, Bintu L, Lehner PJ, 
            Prolo LM, Bassik MC
  TITLE     Reduced Cas9 transgene silencing by incorporation of intron 
            sequences.
  JOURNAL   Nat Commun. 2025 Nov 27;16(1):10656. doi: 
            10.1038/s41467-025-65669-0.
  PUBMED    41309578
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-11-27"; volume: "16(1):10656. doi"; pages: " 
            10.1038/s41467-025-65669-0"
COMMENT     Sequence Label: pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgggaggtgg tccctgcagt tacgccaatg ataacccccg ccagaaaaat cttagtagcc
       61 ttcccttttt gttttccgtg ccccaactcg gcggattgac tcggcccctt ccggaaacac
      121 ccgaatcaac ttctagtcaa attattgttc acgccgcaat gacccacccc tggcccgcgt
      181 ctgtggaact gacccctggt gtacaggaga gttcgctgct gaaagtggtc ccaaaggggt
      241 actagttttt aagctcccaa ctccccctcc cccagcgtct ggaggattcc acaccctcgc
      301 accgcagggg cgaggaagtg ggcggagtcc ggttttggcg ccagccgctg aggctgccaa
      361 gcagaaaagc caccgctgag gagactccgg tcactgtcct cgccccgcct cccccttccc
      421 tccccttggg gaccaccggg cgccacgccg cgaacggtaa gtgccgcggt cgtcggcgcc
      481 tccgccctcc ccctagggcc ccaattccca gcgggcgcgg cgcgcggccc ctccccccgc
      541 cgggcgcgcg cccgctgccc cgcccttcgt ggccgcccgg cgtgggcggt gccacccctc
      601 cccccggcgg ccccgcgcgc agctcccggc tccctccccc ttcggatgtg gcttgagctg
      661 taggcgcgga ggg
//
