LOCUS       sequence-49186-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK3L1
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cttccaaact cctgcgcgcg gtcatcctcg ggccgcccgg ctcgggcaag
      121 ggcaccgtgt gccagaggat cgcccagaac tttggtctcc agcatctctc cagcggccac
      181 ttcttgcggg agaacatcaa ggccagcacc gaagttggtg agatggcaaa gcagtatata
      241 gagaaaagtc ttttggttcc agaccatgtg atcacacgcc taatgatgtc cgagttggag
      301 aacaggcgtg gccagcactg gctccttgat ggttttccta ggacattagg acaagccgaa
      361 gccctggaca aaatctgtga agtggatcta gtgatcagtt tgaatattcc atttgaaaca
      421 cttaaagatc gtctcagccg ccgttggatt caccctccta gcggaagggt atataacctg
      481 gacttcaatc cacctcatgt acatggtatt gatgacgtca ctggtgaacc gttagtccag
      541 caggaggatg ataaacccga agcagttgct gccaggctaa gacagtacaa agacgtggca
      601 aagccagtca ttgaattata caagagccga ggagtgctcc accaattttc cggaacggag
      661 acgaacaaaa tctggcccta cgtttacaca cttttctcaa acaagatcac acctattcag
      721 tccaaagaag catatttgtg acagtaaagg tggatac
//
