LOCUS       sequence-49188-e        1599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1599)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PAK4
FEATURES             Location/Qualifiers
     source          1..1599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     612..634
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             661..684
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
       61 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      121 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      181 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      241 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      301 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      361 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      421 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      481 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      541 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      601 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      661 ctggaagttc tgttccaggg gcccctggga tcctcaccac agcgggagcc acagcgagta
      721 tcccatgagc agttccgggc tgccctgcag ctggtggtgg acccaggcga cccccgctcc
      781 tacctggaca acttcatcaa gattggcgag ggctccacgg gcatcgtgtg catcgccacc
      841 gtgcgcagct cgggcaagct ggtggccgtc aagaagatgg acctgcgcaa gcagcagagg
      901 cgcgagctgc tcttcaacga ggtggtaatc atgagggact accagcacga gaatgtggtg
      961 gagatgtaca acagctacct ggtgggggac gagctctggg tggtcatgga gttcctggaa
     1021 ggaggcgccc tcaccgacat cgtcacccac accaggatga acgaggagca gatcgcggcc
     1081 gtgtgccttg cagtgctgca ggccctgtcg gtgctccacg cccagggcgt catccaccgg
     1141 gacatcaaga gcgactcgat cctgctgacc catgatggca gggtgaagct gtcagacttt
     1201 gggttctgcg cccaggtgag caaggaagtg ccccgaagga agtcgctggt cggcacgccc
     1261 tactggatgg ccccagagct catctcccgc cttccctacg ggccagaggt agacatctgg
     1321 tcgctgggga taatggtgat tgagatggtg gacggagagc ccccctactt caacgagcca
     1381 cccctcaaag ccatgaagat gattcgggac aacctgccac cccgactgaa gaacctgcac
     1441 aaggtgtcgc catccctgaa gggcttcctg gaccgcctgc tggtgcgaga ccctgcccag
     1501 cgggccacgg cagccgagct gctgaagcac ccattcctgg ccaaggcagg gccgcctgcc
     1561 agcatcgtgc ccctcatgcg ccagaaccgc accagatga
//
