LOCUS       sequence-49194-e         517 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 517)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS1
FEATURES             Location/Qualifiers
     source          1..517
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt ctggaatgaa atcttccaag tccaaggatg tactttctgc tgctgaagta
      121 atgcaatggt ctcaatctct ggaaaaactt cttgccaacc aaactggtca aaatgtcttt
      181 ggaagtttcc taaagtctga attcagtgag gagaatattg agttctggct ggcttgtgaa
      241 gactataaga aaacagagtc tgatcttttg ccctgtaaag cagaagagat atataaagca
      301 tttgtgcatt cagatgctgc taaacaaatc aatattgact tccgcactcg agaatctaca
      361 gccaagaaga ttaaagcacc aacccccacg tgttttgatg aagcacaaaa agtcatatat
      421 actcttatgg aaaaggactc ttatcccagg ttcctcaaat cagatattta cttaaatctt
      481 ctaaatgacc tgcaggcttg acagtaaagg tggatac
//
