LOCUS       sequence-49195-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CENTG1
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc gctccattcc tgaactgcgc ctgggtgtgc tgggcgatgc caggagtggg
      121 aagtcatcgc tcatccaccg attcctgact ggctcatacc aggtgctgga gaagacagag
      181 agtgagcagt acaagaaaga aatgttggtg gatggacaga cacatctggt gctaatccga
      241 gaggaagctg gggcacctga tgccaagttc tcaggctggg cagatgctgt gatcttcgtc
      301 ttcagcctgg aggatgagaa cagtttccag gctgtgagcc gtctccatgg gcagctgagt
      361 tcccttcgcg gggagggacg aggaggcctg gccttggcac tggtggggac acaagacagg
      421 atcagtgctt cctcccctcg ggtggtggga gatgctcgtg ccagagctct gtgcgcggac
      481 atgaaacgct gcagctacta tgagacttgt gcaacctatg ggctcaatgt ggatcgggtc
      541 ttccaggagg tggcccagaa ggtggtgacc ttgcgcaagc agcaacaact tctggcttga
      601 cagtaaaggt ggatac
//
