LOCUS       sequence-492004-       10271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  baculovirus-based delivery system that enables the efficient 
            incorporation of unnatural amino acids into proteins in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Chatterjee A, Xiao H, Bollong M, Ai HW, Schultz PG
  TITLE     Efficient viral delivery system for unnatural amino acid mutagenesis
            in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 
            10.1073/pnas.1309584110. Epub 2013 Jul 1.
  PUBMED    23818609
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1309584110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-16- 16"; volume: "110"; issue: "29"; pages: "11803-8"
COMMENT     Sequence Label: pAcBac1.tR4-MbPyl
FEATURES             Location/Qualifiers
     source          1..10271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(4142..4236)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    4142..4160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4170..4188
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(4313..4561)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     polyA_signal    4598..4822
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4811..4828
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    4877..5465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4983..4994
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5392..5412
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      6744..6753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6772..6791)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6773..6791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6836..7039)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6865..6885)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7040..7419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7514..7605
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     7530..7547
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(7664..7711,7712..9199)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    9345..9479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9382..9401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9436..9455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9512..9760
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        9837..9931
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    9885..9903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9913..9931
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     mobile_element  10024..10189
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc gcttaatgcg
     4021 ccgctacagg gcgcgtgggg atacccccta gagccccagc tgaaaaaacg gggggtgcgg
     4081 gagtttcacc cgcctgcacg gatttagagt ccgtgtgatc tattcgatcc accccccggt
     4141 gtctctatca ctgataggga acttataagt ctctatcact gatagggatt tcacgtttat
     4201 ggtgatttcc cagaacacat agcgacatgc aaatattaaa aaacggaaac cccgggaatc
     4261 taacccggct gaacggattt agagtccgtt cgatctacat gatcaggttt ccggtgtttc
     4321 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca
     4381 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
     4441 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
     4501 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
     4561 cttcctgccc gagatctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca
     4621 gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc
     4681 agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg
     4741 acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat
     4801 ggctggcaac tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctctagac
     4861 tcgagttaaa gtcgacgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     4921 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     4981 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     5041 caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg
     5101 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     5161 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     5221 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     5281 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     5341 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     5401 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     5461 tgattgtcga cttaacgcgt tgaattctta cagattggtt gaaatcccat tatagtaaga
     5521 ttcggacctt gatgccctct taatgttttt aaagccgtgc ataaccttga gcaagcgttc
     5581 aagaccaaaa cctgcaccta tccatggttt gtcaataccc cattctctat caagagaaac
     5641 tggcccgacg actgccgaag aaagctccag gtccccgtgc attatatcaa gagtatcccc
     5701 aaagaccata caggaatctc ctacgatttc gaagtcgatt tccagatagt ccagaaactc
     5761 tttgatgaga gcttcaagat tttcccgagt acatcccgaa cccatctgac agaagttcac
     5821 catagtaaat tcttccaggt gctctttgcc gtcagactct ttccggtaac aaggtccgac
     5881 ttcgaaaatt tttattgggc ctggtaaaat cctatcgagt tttcgcagat agttgtaaag
     5941 agtcggggca agcattggcc tcaagcagag atttttatcc acccggaaga tctgttttga
     6001 aagttcagta tcattattaa tacccattct ctccacgtat tccgccggaa taaggatagg
     6061 agactttatc tccagaaaac cccggtctac gaaaaatttc gtaatatcac gttcgagttt
     6121 accgaggtag tcttctctat cattggtata gagccgctga aaatcgtttt ttcttcttgt
     6181 cacaagttca ggctcaagtt ccctgaaagg ctttgccata tttagagaaa ttttatcctc
     6241 tggacttaag agagcctcaa ccctatcaag ctggcttctt gtaagtgaag gagcaggagc
     6301 cgatgcggga acagacgaat ttggagttga ttttgcaggc gaaggtacag atctggatgt
     6361 gttcgttgat gcctttgcag aaacagaatt ttccagaggc ttcggagccc ttgaaactga
     6421 tttcggcata gcttttttga cctttggagc agaaactacc ctaactttca cactgttttt
     6481 gctttcggtt gatcttgtga gaaaattatt gatatcctcg tccgaaaccc tacatcgttt
     6541 gcaggttttt ctgtacttat gatgtctgaa tgctctggct gttctacaac tcctggaatt
     6601 attcacaaca agatggtctc cacacgccat ttcaatgtat attttacttc ttgagacctc
     6661 atggtgcttg attttgtgga gcgtgccagt cctggacatc cagagcccgg tcgcagatat
     6721 taaaacatct aatggttttt tatccatggt ggcgctagcc agcttgggtc tccctatagt
     6781 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
     6841 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     6901 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     6961 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     7021 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     7081 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     7141 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     7201 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     7261 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     7321 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     7381 taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta
     7441 catcgcgaag cagcgcaaaa cggatcctgc aggtatttgc ggccgcggtc cgtatactcc
     7501 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     7561 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     7621 taccgtccca ccatcgggcg cgaactccta aaaaaccgcc accatgaagt gccttttgta
     7681 cttagccttt ttattcattg gggtgaattg caagttcacc atagtttttc cacacaacca
     7741 aaaaggaaac tggaaaaatg ttccttctaa ttaccattat tgcccgtcaa gctcagattt
     7801 aaattggcat aatgacttaa taggcacagc cttacaagtc aaaatgccca agagtcacaa
     7861 ggctattcaa gcagacggtt ggatgtgtca tgcttccaaa tgggtcacta cttgtgattt
     7921 ccgctggtat ggaccgaagt atataacaca ttccatccga tccttcactc catctgtaga
     7981 acaatgcaag gaaagcattg aacaaacgaa acaaggaact tggctgaatc caggcttccc
     8041 tcctcaaagt tgtggatatg caactgtgac ggatgccgaa gcagtgattg tccaggtgac
     8101 tcctcaccat gtgctggttg atgaatacac aggagaatgg gttgattcac agttcatcaa
     8161 cggaaaatgc agcaattaca tatgccccac tgtccataac tctacaacct ggcattctga
     8221 ctataaggtc aaagggctat gtgattctaa cctcatttcc atggacatca ccttcttctc
     8281 agaggacgga gagctatcat ccctgggaaa ggagggcaca gggttcagaa gtaactactt
     8341 tgcttatgaa actggaggca aggcctgcaa aatgcaatac tgcaagcatt ggggagtcag
     8401 actcccatca ggtgtctggt tcgagatggc tgataaggat ctctttgctg cagccagatt
     8461 ccctgaatgc ccagaagggt caagtatctc tgctccatct cagacctcag tggatgtaag
     8521 tctaattcag gacgttgaga ggatcttgga ttattccctc tgccaagaaa cctggagcaa
     8581 aatcagagcg ggtcttccaa tctctccagt ggatctcagc tatcttgctc ctaaaaaccc
     8641 aggaaccggt cctgctttca ccataatcaa tggtacccta aaatactttg agaccagata
     8701 catcagagtc gatattgctg ctccaatcct ctcaagaatg gtcggaatga tcagtggaac
     8761 taccacagaa agggaactgt gggatgactg ggcaccatat gaagacgtgg aaattggacc
     8821 caatggagtt ctgaggacca gttcaggata taagtttcct ttatacatga ttggacatgg
     8881 tatgttggac tccgatcttc atcttagctc aaaggctcag gtgttcgaac atcctcacat
     8941 tcaagacgct gcttcgcaac ttcctgatga tgagagttta ttttttggtg atactgggct
     9001 atccaaaaat ccaatcgagc ttgtagaagg ttggttcagt agttggaaaa gctctattgc
     9061 ctcttttttc tttatcatag ggttaatcat tggactattc ttggttctcc gagttggtat
     9121 ccatctttgc attaaattaa agcacaccaa gaaaagacag atttatacag acatagagat
     9181 gaaccgactt ggaaagtgat aaggccaggc cggccaagct tgtcgagaag tactagagga
     9241 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     9301 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     9361 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9421 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     9481 gatcactgct tgagcctagg tcgggcagga agagggccta tttcccatga ttccttcata
     9541 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
     9601 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     9661 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     9721 tttcttggct ttatatatct tgtggaaagg acgaaacacc ggaaacctga tcatgtagat
     9781 cgaacggact ctaaatccgt tcagccgggt tagattcccg gggtttccgt tttttaatat
     9841 ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatcccta tcagtgatag
     9901 agacttataa gttccctatc agtgatagag acaccggggg gtggatcgaa tagatcacac
     9961 ggactctaaa tccgtgcagg cgggtgaaac tcccgcaccc cccgtttttt gctaggagat
    10021 ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta
    10081 gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa
    10141 aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt
    10201 ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct
    10261 tcggctactt t
//
