LOCUS       sequence-492027-        9152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a minimal promoter only with single TATA 
            cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor 
            lentiviral transduction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE60
FEATURES             Location/Qualifiers
     source          1..9152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1984..2015
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2042..2051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2048..3697
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3701..3820
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3912..4500
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3965..3985)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4383..4394)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4515..4831
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4682..4817
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4882..4884,4885..4887,4888..5601)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4927..4948)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5188..5207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5535..5556
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5673..5906
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5978..6099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6015..6034)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6069..6088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6139..6274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6294..6313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6295..6313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6323..6340)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6323..6339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6332..6354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6481..6936
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6568..6587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6778..6799
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6962..7066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7067..7135,7136..7927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7285..7304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8098..8686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8587..8606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8840..8857
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8974..8995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9010..9015,9016..9033,9034..9040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9048..9064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9053..9075
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9072..9088
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9072..9088
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9107..9127
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9109..9127
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatccgaga acagatctgg cctcggcggc caagcttaga
     1981 cactagaggg tatataatgg aagctcgact tccagcttgg caatccggta ctgttggtaa
     2041 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
     2101 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
     2161 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
     2221 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
     2281 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
     2341 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
     2401 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
     2461 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
     2521 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
     2581 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
     2641 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
     2701 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
     2761 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
     2821 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     2881 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     2941 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     3001 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     3061 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     3121 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     3181 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     3241 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     3301 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     3361 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     3421 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     3481 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     3541 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     3601 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     3661 tctcattaag gccaagaagg gcggcaagat cgccgtgaat tctcacggct tccctcccga
     3721 ggtggaggag caggccgccg gcaccctgcc catgagctgc gcccaggaga gcggcatgga
     3781 tagacaccct gctgcttgcg ccagcgccag gatcaacgtc taaggccgcg actctagaac
     3841 gcgtcactgc ggcttttcaa cggggtagac cgactacaga catcagcgcg taccggttag
     3901 taatgatcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3961 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4021 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4081 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4141 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4201 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4261 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4321 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4381 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4441 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4501 ctggcgatgg taccggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4561 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4621 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4681 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4741 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     4801 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     4861 cggatctgat cagcacctag catggtgagc aagggcgagg agctgttcac cggggtggtg
     4921 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4981 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     5041 ctgcccgtgc cctggcccac cctcgtgacc accttcacct acggcgtgca gtgcttcgcc
     5101 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5161 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5221 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5281 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc
     5341 accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag
     5401 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5461 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     5521 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5581 atggacgagc tgtacaagta aggtaccttt aagaccaatg acttacaagg cagctgtaga
     5641 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5701 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5761 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5821 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5881 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5941 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6121 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6241 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6301 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6781 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6841 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6901 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6961 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7021 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7081 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7141 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7201 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7261 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7321 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7381 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7441 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7501 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7561 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7621 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7681 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7741 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7801 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7861 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7921 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7981 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8461 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8641 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8701 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8761 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8821 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8881 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8941 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9001 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9061 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9121 ctaaagggaa caaaagctgg agctgcaagc tt
//
