LOCUS       sequence-492045-        5752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insert a C-terminal moxGFP2 at GNT1 by popin-popout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5752)
  TITLE     Glick lab plasmids 2025
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YIplac211-Gnt1-moxGFP2
FEATURES             Location/Qualifiers
     source          1..5752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2250..2267)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2250..2266)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2259..2281)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2475..2494)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2594..2616
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2734..3537)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3538..3753)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(3760..3778)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3846..3950
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3951..4019,4020..4811)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4169..4188)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4982..5570
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5471..5490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5724..5741
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctgga
      241 cattataagt tctacatcat ctttatcgcc gttatttgat ttccaaagta gtaaaaaggt
      301 tggaaaatta aaatttgctt ctaatttaat ggttattaat ccttcaaagg aggcgtttga
      361 tgaaattgtg aacgttatgc tgcccaagat tttaaacaag aaggaaaagt acgatatgga
      421 tttgatcaat gaagaaatgt acaatttaaa gaagattatt tataaacagt ttattttctt
      481 cagaaaagtc cgaaaacttt tcaaaccaga agtattggtt ttgccatttg caaggtacgg
      541 cttactgacg ggttcattga gaaacccacg gcactattct ataatctata atgacgtttt
      601 gggttataag acattggata atgatggcaa cgatattccg gtaggattaa acgacagtgt
      661 cgcatattct aagtacatac atttttcgga ttatccattg gccaagccct ggaattatcc
      721 atcaatgaag gagtttgaat gtatcgttaa agaagaagac gcggaggatt caaagctaga
      781 gcaccaagct tgtgaccttt ggaactcggt gtacgcttct tacatacagt ccagggaaat
      841 ctgcctcgtt ggaagttcga tggatagcac tgagagcttg ttcacaggtg ttgttcctat
      901 cttggtcgaa ttagacggtg acgtaaacgg tcataagttc agtgttagag gtgaaggtga
      961 aggtgacgct actaacggta aattgacatt gaagtttatt agtactacag gtaaattgcc
     1021 agttccttgg ccaactttgg ttactacatt gacatacggt gttcaagttt tttcaagata
     1081 cccagatcat atgaagcaac atgatttctt taagtctgct atgccagaag gttacgttca
     1141 agaaagaact atcactttta aagatgatgg tacttacaag acaagagcag aggttaagtt
     1201 cgaaggtgac actttggtta acagaatcga attgaagggt atcgatttta aagaagatgg
     1261 taatattttg ggtcataaat tggaatacaa ctacaactca cataacgttt acattacagc
     1321 tgataaacaa aagaatggta ttaaagcaaa cttcaagatc agacataatg ttgaagatgg
     1381 ttctgttcaa ttagcagatc attaccaaca aaacactcca attggtgacg gtccagtttt
     1441 gttaccagat aaccattact tgtctacaca atcaaaattg tctaaagatc caaacgaaaa
     1501 gagagatcat atggttttgt tagaatttgt tactgctgct ggtatcacat gattatgagg
     1561 agaagtgaaa attttcaaaa agtgtaaaaa ttagtatagt cggaaacgca tatatatata
     1621 tcatagtatt gataatgttt ctgccaagat tgtgtccgtt atcgcatttt ctaacgggta
     1681 aggtatgtac taggtgagag cgggatctta tttcaacatg ttgtgcttta atgcttcttt
     1741 attcaacagc ctgtcaacgt catcaccgta ttttgcgtta ccttttccat tttctttgaa
     1801 ggcataatca acagtaattc tgttgttggc gactaactga ttgttcaaag attttattgc
     1861 caaatctgct ttttcaaagt cctcaaaata aacataagca cactttaatt ttccgtttga
     1921 taggtagaaa atttcgggtt cccttatgag cttaccaaat ttattgaaaa ttttcactaa
     1981 ttggtcgcta tctatcgaat ctgcaaggtt ttttatgaac aacttagcaa taggtagaat
     2041 catgtcctta gaaatatttg aaggtaaatt agttgttcct gtagaattgg ttacttgacg
     2101 aacctttatc agtcggtcgt ataatctaac ggtattattc attattttta ttgcatattg
     2161 agcatctcct tgattataaa attcgatgaa tgcgtagcct tggtaagctt gtagaaccta
     2221 gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg
     2281 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2341 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2401 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2461 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     2521 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2581 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2641 gcgagacgaa agggcgggta ataactgata taattaaatt gaagctctaa tttgtgagtt
     2701 tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa
     2761 atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt
     2821 tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca catcatccac
     2881 ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat
     2941 aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat
     3001 aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga
     3061 tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac
     3121 ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt
     3181 cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt
     3241 attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa
     3301 tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt
     3361 ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt
     3421 acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt
     3481 ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat
     3541 ttatcttcgt ttcctgcatg tttttgttct gtgcagttgg gttaagaata ctgggcaatt
     3601 tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct
     3661 tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca
     3721 aaaaaaagaa taaaaaaaaa atgatgaatt gaaaagctgg cctcgtgata cgcctatttt
     3781 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3841 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3901 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3961 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4021 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4081 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4141 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4201 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4261 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4321 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4381 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4441 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4501 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4561 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4621 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4681 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4741 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4801 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4861 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4921 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4981 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5041 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5101 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5161 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5221 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5281 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5341 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5401 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5461 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5521 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5581 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5641 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5701 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
