LOCUS       sequence-49207-e        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD17B4
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             604..615
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gcagcccgct gcgttttgat ggccgtgtgg tgctggtgac cggcgcgggc
      121 gcgggcctgg gccgtgcgta tgcgctggcg tttgcggaac gtggcgcgct ggtggtggtg
      181 aacgatctgg gcggtgattt taaaggcgtg ggcaaaggca gcctggcggc cgataaagtg
      241 gtggaagaaa ttcgtcgtcg tggcggtaaa gcggtggcga actatgatag cgtggaagaa
      301 ggcgaaaaag tggtgaaaac cgcgctggat gcgtttggcc gtattgatgt ggtggtgaac
      361 aatgcgggca ttctgcgtga tcgtagcttt gcgcgtatta gcgatgaaga ttgggatatt
      421 atccatcgtg tgcatctgcg tggcagcttt caggtgaccc gtgcggcctg ggaacacatg
      481 aaaaaacaga aatatggccg tattatcatg accagcagcg cgagcggcat ttatggcaac
      541 tttggccagg cgaactatag cgcggccaaa ctgggcctgc tgggcctggc gaacagcctg
      601 gcgattgaag gccgtaaaag caacattcat tgcaacacca ttgcgccgaa cgcgggcagc
      661 cgtatgaccc agaccgtgat gccggaagat ctggtggaag cgctgaaacc ggaatatgtg
      721 gcgccgctgg tgctgtggct gtgccatgaa agctgcgaag aaaacggcgg tctgtttgaa
      781 gtgggcgcgg gctggattgg caaactgcgt tgggaacgta ccctgggcgc gattgtgcgt
      841 cagaaaaacc atccgatgac cccggaagcg gtgaaagcga actggaaaaa aatttgcgat
      901 tttgaaaacg cgagcaaacc gcagagcatt caggaaagca ccggcagcat tatcgaagtg
      961 ctgagcaaaa ttgatagcga aggcggtgtg agcgcgaacc ataccagccg tgcgaccagc
     1021 accgcgtgac agtaaaggtg gatac
//
