LOCUS       sequence-492098-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses an NGI-resistant version of STT3A, STT3A Q260R, in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Lampson BL, Ramiotarez AS, Baro M, He L, Hegde M, Koduri V, Pfaff 
            JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, 
            Locher KP, Kaelin WG Jr
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an 
            oligosaccharyltransferase required for inflammatory signaling to 
            NF-kappaB.
  JOURNAL   Cell. 2024 Apr 25;187(9):2209-2223.e16. doi: 
            10.1016/j.cell.2024.03.022.
  PUBMED    38670073
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.03"; journalName: "Cell"; date: "2024-04-25- 
            25"; volume: "187"; issue: "9"; pages: "2209-2223.e16"
COMMENT     Sequence Label: STT3A Q260R
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     485..505
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     531..555
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2877..3376
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2912..2935)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3309..3328
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3397..3996
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3397..3416)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3893..3913
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4010..4598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4063..4083)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4481..4492)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4805..5438
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5567..5583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5567..5583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5580..5602)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5591..5607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5615..5621,5622..5639,5640..5645))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5660..5681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5798..5815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5969..6557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6049..6068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6728..7519,7520..7588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7351..7370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7589..7693)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7741..7875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7778..7797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7832..7851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8043..8676
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8723..8848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9345..9578
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9763..9807
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10070..10185
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gtttatcgat aagcttggga gttccgcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgactctact agaggatcgc tagcgctacc ggactcagat ctcgagctca tcgggatccg
      661 ccaccatgac taagtttgga tttttgcgat tgtcctatga gaagcaggac acacttttga
      721 agcttctcat tctgtcaatg gctgctgtat tatccttctc cactcgtctg tttgctgtcc
      781 tgagatttga aagtgttatc catgagtttg atccgtactt taattatcgg actaccaggt
      841 tcctggctga ggaggggttt tataaattcc ataactggtt tgatgaccga gcctggtacc
      901 ctttgggacg aatcattgga ggaacaattt acccaggttt aatgatcacc tctgctgcaa
      961 tctaccatgt actccatttt ttccacatca ccatcgacat tcggaatgtc tgtgtgttcc
     1021 tggcccctct cttctcctcc ttcacaacga tcgtcacgta ccaccttacc aaagagctca
     1081 aggatgcagg ggctgggctt cttgctgctg ccatgattgc tgtagttcct ggatatatct
     1141 cccgatctgt ggctggctcc tatgataatg aagggattgc catcttttgc atgctactca
     1201 cctactacat gtggatcaag gcagtaaaga ctggttccat ctgttgggca gctaagtgtg
     1261 cccttgctta tttctacatg gtctcgtcat ggggaggtta tgtgttcctg atcaacttaa
     1321 ttcctctcca cgtcctcgtg ctgatgctca caggccgttt ctctcaccgg atctatgtgg
     1381 cctactgtac tgtttactgc ctgggcacta tactttctat gcagatctcc tttgtgggtt
     1441 tccggcctgt cctttcatca gagcacatgg cagcctttgg ggtctttggt ctctgccaga
     1501 tccatgcctt tgtggattac ctgcgcagca agttgaatcc acaacaattt gaagttcttt
     1561 tccggagcgt catctctctg gtaggctttg tccttctcac cgtgggagct ctcctcatgc
     1621 tgacaggaaa aatatctccc tggacggggc gtttctactc gctgctagat ccctcttatg
     1681 ctaagaacaa catccccatc attgcttctg tgtctgagca tcagcccaca acctggtcct
     1741 catactattt tgacctgcag ctactcgtct tcatgtttcc agttggtctg tattactgct
     1801 ttagcaacct gtctgatgcc cggattttta tcatcatgta tggtgtgacc agcatgtact
     1861 tttcagctgt aatggtgcgt ctaatgctag tgttggcacc tgttatgtgc attctctctg
     1921 gcattggagt ctcccaggtg ctgtccacat acatgaagaa tctggacata agtcgtccag
     1981 acaagaagag caagaagcaa caggattcca cctaccctat taagaatgaa gtggcaagtg
     2041 ggatgatact ggtcatggct ttctttctca tcacctacac ctttcattca acctgggtga
     2101 ccagtgaggc ctactcttct ccgtccattg tactatctgc ccgtggtggg gatggcagta
     2161 ggatcatatt tgatgacttc cgagaagcat attattggct tcgtcataat actccagagg
     2221 atgcgaaggt catgtcctgg tgggattatg gctatcagat tacagctatg gcaaaccgaa
     2281 caattttagt ggacaataac acatggaata atacccatat ttctcgagta gggcaggcaa
     2341 tggcgtccac agaggaaaaa gcctatgaga tcatgaggga gctcgatgtc agctatgtgc
     2401 tggtcatttt tggaggcctc actgggtatt cctctgatga tatcaacaag tttctttgga
     2461 tggtccggat tggagggagc acagatacag gcaaacatat caaggagaat gactattata
     2521 ctccaactgg ggagttccgt gtggaccgtg aaggttctcc agtgctgctc aactgcctca
     2581 tgtacaagat gtgttactat cgctttggac aggtttacac agaagccaag cgtcctccag
     2641 gctttgaccg tgtccgaaat gctgagattg ggaataaaga ctttgagctt gatgtcctgg
     2701 aggaagcata taccacagaa cattggctgg tcaggatata caaggtaaag gacctggata
     2761 atcgaggctt gtcaaggaca ggctcgggca gcggtggctc tggaggttct gtgagcggct
     2821 ggcggctgtt caagaagatt agctaagaat tccgacatgc tctagataat tctaccgggt
     2881 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     2941 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     3001 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     3061 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     3121 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     3181 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     3241 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     3301 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     3361 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     3421 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     3481 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     3541 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     3601 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     3661 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     3721 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     3781 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     3841 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     3901 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     3961 cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct
     4021 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4081 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4141 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4201 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4261 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4321 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4381 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4441 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4501 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4561 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     4621 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     4681 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     4741 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     4801 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     4861 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     4921 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     4981 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     5041 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     5101 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     5161 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     5221 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     5281 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5341 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5401 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5461 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     5521 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     5581 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5641 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5701 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5761 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5821 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5881 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5941 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6001 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6061 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6121 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6181 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6241 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6301 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6361 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6421 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6481 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6541 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6601 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6661 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6721 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6781 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6841 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6901 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6961 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7021 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7081 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7141 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7201 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7261 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7321 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7381 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7441 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7501 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7561 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7621 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7681 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7741 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7801 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7861 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     7921 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     7981 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     8041 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     8101 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     8161 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     8221 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     8281 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     8341 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     8401 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     8461 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     8521 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8581 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8641 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     8701 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     8761 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     8821 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     8881 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     8941 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     9001 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     9061 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     9121 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     9181 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     9241 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     9301 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     9361 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     9421 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     9481 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     9541 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     9601 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     9661 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     9721 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     9781 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     9841 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     9901 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     9961 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    10021 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa
    10081 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    10141 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    10201 ggacagcaga gatcca
//
