LOCUS       sequence-49212-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SPR
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc tgggtcgtgc ggtgtgcctg ctgaccggtg cgagccgtgg ttttggtcgt
      121 accctggcgc cgctgctggc gagcctgctg agcccgggta gcgtgctggt gctgagcgcg
      181 cgtaacgatg aagcgctgcg tcagctggaa gcggaactgg gtgcggaacg tagcggtctg
      241 cgtgtggtgc gtgtgccggc ggatctgggt gcggaagcgg gtctgcagca gctgctgggt
      301 gcgctgcgtg aactgccgcg tccgaaaggc ctgcagcgtc tgctgctgat taataatgcg
      361 ggcagcctgg gcgatgtgag caaaggcttt gtggatctga gcgatagcac ccaggtgaac
      421 aattattggg cgctgaacct gaccagcatg ctgtgcctga ccagcagcgt gctgaaagcg
      481 tttccggata gcccgggcct gaaccgtacc gtggtgaata ttagcagcct gtgcgcgctg
      541 cagccgttta aaggttgggc gctgtattgc gcgggcaaag cggcccgtga tatgctgttt
      601 caggtgctgg cgctggaaga accgaatgtg cgtgtgctga attatgcgcc gggtccgctg
      661 gataccgata tgcagcagct ggcgcgtgaa accagcgttg atccggatat gcgtaaaggc
      721 ctgcaggaac tgaaagcgaa aggcaaactg gtggattgca aagtgagcgc gcagaaactg
      781 ctgagcctgc tggaaaaaga tgaatttaaa agcggcgcgc atgtggattt ttatgataaa
      841 tgacagtaaa ggtggatac
//
