LOCUS       sequence-492174-       10958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DOX-suppressible POLD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10958)
  TITLE     Targeting DNA polymerase epsilon leads to tumor clearance and 
            activation of an NF-_B-mediated inflammatory response in triple 
            negative breast cancer
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW57.1 DOX off blast POLD1
FEATURES             Location/Qualifiers
     source          1..10958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             599..664
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(1478..1489)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        4095..4605
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4524..4542
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4615..5007
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5017..5070
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5071..5688,5689..5694,5695..5730,5731..5733,5734..5769,
                     5770..5772,5773..5808,5809..5817)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5857..5877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6275..6286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6491..6724
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6796..6917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6833..6852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6887..6906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6957..7092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7092..7163
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7164..7232,7233..8024)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7382..7401)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8195..8783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8684..8703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8937..8954
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9071..9092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9107..9112,9113..9130,9131..9137)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9145..9161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9150..9172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9169..9185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9169..9185
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9204..9224
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9206..9224
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9252..9478
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9479..9659
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9706..9831
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10324..10557
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10742..10786
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcctagactg ccggatctag ctagttaatt aaggatccct aaatcgcaat tgaccatgga
      601 ctacaaggac cacgacggtg actacaagga ccacgacatc gactacaagg acgacgacga
      661 caagggagga ggcggaggtg gaatggatgg caagcggcgg ccaggcccag ggcccggggt
      721 gcccccaaag cgggcccgtg ggggcctctg ggatgatgat gatgcacctc ggccatccca
      781 attcgaggag gacctggcac tgatggagga gatggaggca gaacacaggc tgcaggagca
      841 ggaggaggag gagctgcagt cagtcctgga gggggttgca gacgggcagg tcccaccatc
      901 agccatagat cctcgctggc ttcggcccac accaccagcg ctggaccccc agacagagcc
      961 cctcatcttc caacagttgg agattgacca ttatgtgggc ccagcacagc ctgtgcctgg
     1021 ggggccccca ccatcccacg gctccgtgcc tgtgctccgc gccttcgggg tcaccgatga
     1081 ggggttctct gtctgctgcc acatccacgg cttcgctccc tacttctaca ccccagcgcc
     1141 ccctggtttc gggcccgagc acatgggtga cctgcaacgg gagctgaact tggccatcag
     1201 ccgggacagt cgcgggggga gggagctgac tgggccggcc gtgctggctg tggaactgtg
     1261 ctcccgagag agcatgtttg ggtaccacgg gcacggcccc tccccgttcc tgcgcatcac
     1321 cgtggcgctg ccgcgcctcg tggccccggc ccgccgtctc ctggaacagg gcatccgtgt
     1381 ggcaggcctg ggcacgccca gcttcgcgcc ctacgaggcc aacgtcgact ttgagatccg
     1441 gttcatggtg gacacggaca tcgtcggctg caactggctg gagctcccag ctgggaaata
     1501 cgccctgagg ctgaaggaga aggctacgca gtgccagctg gaggcggacg tgctgtggtc
     1561 tgacgtggtc agtcacccac cggaagggcc atggcagcgc attgcgccct tgcgcgtgct
     1621 cagcttcgat atcgagtgcg ccggccgcaa aggcatcttc cctgagcctg agcgggaccc
     1681 tgtcatccag atctgctcgc tgggcctgcg ctggggggag ccggagccct tcctacgcct
     1741 ggcgctcacc ctgcggccct gtgcccccat cctgggtgcc aaggtgcaga gctacgagaa
     1801 ggaggaggac ctgctgcagg cctggtccac cttcatccgt atcatggacc ccgacgtgat
     1861 caccggttac aacatccaga acttcgacct tccgtacctc atctctcggg cccagaccct
     1921 caaggtacaa acattccctt tcctgggccg tgtggccggc ctttgctcca acatccggga
     1981 ctcttcattc cagtccaagc agacgggccg gcgggacacc aaggttgtca gcatggtggg
     2041 ccgcgtgcag atggacatgc tgcaggtgct gctgcgggag tacaagctcc gctcctacac
     2101 gctcaatgcc gtgagcttcc acttcctggg cgagcagaag gaggacgtgc agcacagcat
     2161 catcaccgac ctgcagaatg ggaacgacca gacccgccgc cgcctggctg tgtactgcct
     2221 gaaggatgcc tacctgccac tgcggctgct ggagcggctc atggtgctgg tgaacgccgt
     2281 ggagatggcg agggtcactg gcgtgcccct cagctacctg ctcagtcgtg gccagcaggt
     2341 caaggtcgta tcccagctgt tgcggcaggc catgcacgag gggctgctga tgcccgtggt
     2401 gaagtcagag ggcggcgagg actacacggg agccactgtc atcgagcccc tcaaagggta
     2461 ctacgacgtc cccatcgcca ccctggactt ctcctcgctg tacccgtcca tcatgatggc
     2521 ccacaacctg tgttacacca cgctccttcg gcccgggact gcacagaaac tgggcctgac
     2581 tgaggatcag ttcatcagga cccccaccgg ggacgagttt gtgaagacct cagtgcggaa
     2641 ggggctgctg ccccagatcc tggagaacct gctcagtgcc cggaagaggg ccaaggccga
     2701 gctggccaag gagacagacc ccctccggcg ccaggtcctg gatggacggc agctggcgct
     2761 gaaggtgagc gccaactccg tatacggctt cactggcgcc caggtgggca agttgccgtg
     2821 cctggagatc tcacagagcg tcacggggtt cggacgtcag atgatcgaga aaaccaagca
     2881 gctggtggag tctaagtaca cagtggagaa tggctacagc accagtgcca aggtggtgta
     2941 tggtgacact gactccgtca tgtgccgatt cggcgtgtcc tcggtggctg aggcgatggc
     3001 cctggggcgg gaggccgcgg actgggtgtc aggtcacttc ccgtcgccca tccggctgga
     3061 gtttgagaag gtctacttcc catacctgct tatcagcaag aagcgctacg cgggcctgct
     3121 cttctcctcc cggcccgacg cccacgaccg catggactgc aagggcctgg aggccgtgcg
     3181 cagggacaac tgccccctcg tggccaacct ggtcactgcc tcactgcgcc gcctgctcat
     3241 cgaccgagac cctgagggcg cggtggctca cgcacaggac gtcatctcgg acctgctgtg
     3301 caaccgcatc gatatcagcc agctcgtgat tactaaagag ctcaccagag ccgcttcaga
     3361 ttacgcggga aaacaagccc atgtcgaact ggcagaacgg atgagaaaaa gagatccagg
     3421 gagcgctccc tcactcgggg atagagtccc ctatgtaatc atttcagcag ccaagggggt
     3481 agccgcctac atgaaatcag aggacccttt gttcgtgctg gaacacagtc ttcccattga
     3541 tacacagtac tacttggaac agcagttggc taaaccgctg ctgcggatct ttgaaccaat
     3601 tttgggcgaa ggtagggcag aggcagtatt gctccggggt gatcatacgc gctgcaaaac
     3661 ggtactcact ggcaaagttg gtggcctgct cgcgttcgca aagagacgaa actgctgtat
     3721 cgggtgtagg acagtgctca gccatcaggg agcagtctgt gaattttgcc agcccaggga
     3781 gagcgagctt tatcagaaag aggtctctca cctcaacgct ctggaggaga gattttccag
     3841 actgtggact cagtgtcagc ggtgtcaagg aagcttgcac gaggatgtaa tttgtacttc
     3901 tcgcgattgc cccatctttt atatgagaaa aaaggttcgg aaggatctcg aagatcaaga
     3961 gcagctcctc agacgatttg gccctcctgg acccgaagcc tggctcgacg aattcggatc
     4021 cctgcagcct atggccactt tggtagggac agcttcccat gggaagtgcc caaaaagctt
     4081 aaatattgag gtccggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     4141 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     4201 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     4261 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     4321 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     4381 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     4441 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     4501 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     4561 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagcaatt caccatggtt
     4621 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     4681 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     4741 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     4801 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     4861 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     4921 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     4981 ctgccctctg gttatgtgtg ggagggcccc ggtgccgaag gtagaggttc tctcctcact
     5041 tgtggtgatg ttgaagaaaa ccctggtcca atgtctagac tggacaagag caaagtcata
     5101 aactctgctc tggaattact caatgaagtc ggtatcgaag gcctgacgac aaggaaactc
     5161 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     5221 ctgctcgatg ccctggcaat cgagatgctg gacaggcatc atacccactt ctgccccctg
     5281 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc
     5341 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     5401 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     5461 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggatcaggag
     5521 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     5581 agacaagcaa ttgagctgtt cgaccatcag ggagccgaac ctgccttcct tttcggcctg
     5641 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac
     5701 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     5761 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta
     5821 agtaaggatc gatccaagat atcgtattct taactatgtt gctcctttta cgctatgtgg
     5881 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5941 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6001 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6061 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6121 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6181 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     6241 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6301 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6361 gagtcggatc tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc cggactagag
     6421 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6481 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6721 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6781 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6901 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     6961 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7021 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7081 tttttggagg cctttcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7141 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7201 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7261 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7321 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7381 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7441 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7501 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7561 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7621 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7681 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7741 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7801 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7861 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7921 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7981 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8101 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8221 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8281 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8341 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8401 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8461 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8521 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8581 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8641 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8701 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8761 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8821 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8881 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8941 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9001 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9061 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9181 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9241 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     9301 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     9361 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     9421 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     9481 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9541 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9601 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9661 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9721 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9781 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9841 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9901 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9961 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    10021 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    10081 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    10141 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
    10201 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    10261 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    10321 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    10381 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    10441 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    10501 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10561 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10621 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10681 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10741 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10801 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10861 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10921 attatcgttt cagacccacc tcccaacccc gaggggac
//
