LOCUS       sequence-49223-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MST4
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cccactcgcc ggtggctgtc caagtgcctg ggatgcagaa taacatagct
      121 gatccagaag aactgttcac aaaattagag cgcattggga aaggctcatt tggggaagtt
      181 ttcaaaggaa ttgataaccg tacccagcaa gtcgttgcta ttaaaatcat agaccttgag
      241 gaagccgaag atgaaataga agacattcag caagaaataa ctgtcttgag tcaatgtgac
      301 agctcatatg taacaaaata ctatgggtca tatttaaagg ggtctaaatt atggataata
      361 atggaatacc tgggcggtgg ttcagcactg gatcttcttc gagctggtcc atttgatgag
      421 ttccagattg ctaccatgct aaaggaaatt ttaaaaggtc tggactatct gcattcagaa
      481 aagaaaattc accgagacat aaaagctgcc aatgtcttgc tctcagaaca aggagatgtt
      541 aaacttgctg attttggagt tgctggtcag ctgacagata cacagattaa aagaaatacc
      601 tttgtgggaa ctccattttg gatggctcct gaagttattc aacagtcagc ttatgactca
      661 aaagctgaca tttggtcatt gggaattact gctattgaac tagccaaggg agagccacct
      721 aactccgata tgcatccaat gagagttctg tttcttattc ccaaaaacaa tcctccaact
      781 cttgttggag actttactaa gtcttttaag gagtttattg atgcttgcct gaacaaagat
      841 ccatcatttc gtcctacagc aaaagaactt ctgaaacaca aattcattgt aaaaaattca
      901 aagaagactt cttatctgac tgaactgata gatcgtttta agagatggaa ggcagaaggg
      961 cacagttgac agtaaaggtg gatac
//
