LOCUS       sequence-49224-e         436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 436)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GHRHR
FEATURES             Location/Qualifiers
     source          1..436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             73..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..135
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atggtaagcg ctattgtttt atatgtgctt ttggcggcgg cggcgcattc tgcctttgcg
       61 gccgccatgg gccaccatca tcatcatcat tcttctggtg tagatctggg taccgagaac
      121 ctgtacttcc aatccatgct gagagaggat gagagtgcct gtctacaagc agcagaggag
      181 atgccccaaa ccaccctggg ctgccctgcg acctgggatg ggctgctgtg ctggccaacg
      241 gcaggctctg gcgagtgggt caccctcccc tgcccggatt tcttctctca cttcagctca
      301 gagtcagggg ctgtgaaacg ggattgtact atcactggct ggtctgagcc ctttccacct
      361 taccctgtgg cctgccctgt gcctctggag ctgctggctg aggaggaatg acagtaaagg
      421 tggatacgga tccgaa
//
