LOCUS       sequence-492308-        9990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  snuABE4.1 with MLH1-SB fused to the C-terminus via P2A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Im HW, Jeong B, Lee Y, Oh YE, CJung C, Kim YW, Uhm H, Bae S
  TITLE     Engineered ADARs enable single-nucleotide resolution DNA A-to-G 
            editing without bystander effects
  JOURNAL   bioRxiv 2025.07.19.664718
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.19.664718"
COMMENT     Sequence Label: snuABE4.2
FEATURES             Location/Qualifiers
     source          1..9990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(765..785)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2061..2081)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2130..2149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2131..2149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2191..2394)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2220..2240)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2395..2774
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2946..2965
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3044..3148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3149..3217,3218..4009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3367..3386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4180..4768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4669..4688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4922..4939
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5026..5137
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5126..5143
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5166..5183)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5445..5501)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5511..5537)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(5796..5849)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5850..5876)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(5886..5906)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(6630..9990,1..740))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 aggcccaggc tcagggcaat caggtttccg aacaggccat tcttcttctc gccgggcagc
       61 tgggcgatca gattttccag ctttctgctc ttgctcagtc tggcagacag gatggccttg
      121 gcgtccacgc cgctggcgtt gatggggttt tcctcgaaca gctggttgta ggtctgcacc
      181 agctggatga acagcttgtc cacgtcgctg ttgtcggggt tcaggtcgcc ctcgatcagg
      241 aagtggcccc ggaacttgat catgtgggcc agggccagat agatcagccg caggtcggcc
      301 ttgtcggtgc tgtccaccag tttctttctc aggtggtaga tggtggggta cttctcgtgg
      361 taggccacct cgtccacgat gttgccgaag atggggtgcc gctcgtgctt cttatcctct
      421 tccaccagga aggactcttc cagtctgtgg aagaagctgt cgtccacctt ggccatctcg
      481 ttgctgaaga tctcttgcag atagcagatc cggttcttcc gtctggtgta tcttcttctg
      541 gcggttctct tcagccgggt ggcctcggct gtttcgccgc tgtcgaacag cagggctccg
      601 atcaggttct tcttgatgct gtgccggtcg gtgttgccca gcaccttgaa tttcttgctg
      661 ggcaccttgt actcgtcggt gatcacggcc cagcccacag agttggtgcc gatgtccagg
      721 ccgatgctgt acttcttgtc gcttccgccg ctagagcctc cgctcacctt tcttttcttc
      781 ttagggctct cgaattcaga gccgtcggcg gtccgcttgc tgccaccaga gcttccgccg
      841 gagctgatgc ttccatagct gctgttataa atgatatgtt tcagcaagct gtcctcgtcc
      901 cactcgaact ggtccacctc ggggggcttt ttcatccaga agcccagctt ggccttagaa
      961 aagcccttca gcagggccat tttagcggcc tgaaatcctg tagccttctc tttgcatttg
     1021 ttgtacatca gaggctcgtt ggtggcgtgg atattggtga tggtggggat ttttcccagc
     1081 agtctcagga accggtagaa cagcttgcac ttgctgatcc gggagatgtg gtccaggtcg
     1141 gttctgcctg tgatggcgtt cacgatttcg gcttcaggtt cgccgtagtt ccagttgatg
     1201 ctgaatgaag ggctcttgcc cacctgtctg gcctcggtat tgctgatggc gcccagctta
     1261 ggcttgttca gtctgtatgg aggggtcagg tcgtagatct cgttctcgat tctgccgtac
     1321 acggccctgt acaggtggct ggggtggaac aggctgccca ggatgataga atccaggtac
     1381 acaggttgga tgaagttgct caggagagcg ccctggacac ccaccacatt ccacttagcc
     1441 agcttatcgc tacaagacat tgttagcagt ctctcgccca ccagcacgcc atcccaggtc
     1501 tggatcagat cggagctatt aacaggaatg gtgccctgtc ctctctcgat cttggtccgc
     1561 agctggcccc gggcctttct attagggtgc ttgtcgaaga tacacacgtc gtgggggcta
     1621 aacagtctgg agtcgccaca aggggcgctg gtgatgtaca ggtggaactt gtacttgggg
     1681 tcgatcagga agcccttgtt tggggacttc agaaagatgc tgttgcgtcc ggtgtcgtcc
     1741 ttcacgtact tttccagctc gctgtagaag tagttgcaca ggcaccgtct gctgatgatc
     1801 tcggcgtgag agtcgttgag gacggagccg ttggcagaaa ttctatcgcc gctgatgcat
     1861 tttgtgccgg tggacactgt caggaccttg caagattcca gattgtaatc ctcgctcatc
     1921 acgatgccgg ccagcactct ccgccggctg taggcagggt tgttcttaat cagctcgccg
     1981 aacttcttgt tgatcagtgt agccacgtga tcggcgaaga tctgaggcac gctttccatc
     2041 ttggcgtcca ggcaggccag gactttccgc ttcttctttg gtgactcgaa ctcgcttccg
     2101 tcggctgtcc gtttcatggt ggcggctctc cctatagtga gtcgtattag cggccgcgga
     2161 tctctagcgg atctgacggt tcactaaacc agctctgctt atatagacct cccaccgtac
     2221 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
     2281 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
     2341 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
     2401 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
     2461 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
     2521 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
     2581 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
     2641 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
     2701 gaactcaata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
     2761 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
     2821 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat
     2881 tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa
     2941 ctatgcggca tcagagcaga ttgtactgag agtagaccct aggggatcgg gagatcgatc
     3001 tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3061 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3121 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3181 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3241 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3301 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3361 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3421 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3481 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3541 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3601 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3661 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3721 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3781 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3841 taaatctgga gccggtgagc gtggttctcg cggtatcatt gcagcactgg ggccagatgg
     3901 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3961 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4141 ctgagtgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4321 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4801 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4861 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4921 cagcgagtca gtgagcgagg actgctattc tcttcccaat cctccccctt gctgtcctgc
     4981 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aattttctca
     5041 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     5101 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
     5161 actcaatggt gatggtgatg atgaccggtt tactcaagtt tctcaaattt ttccaagatc
     5221 ttcttcttaa aagcctccca aatctcctcc ttattcttcc acttcccctc agctttcaac
     5281 tcctttttgg cctcctcgac aatttctttg gcgagcttag cgagttcttc tgtctccttt
     5341 ttctcttttt cttcttcctc tttccgcttc ttcttcaaat tctccctcag gcgtcgcatc
     5401 ccttcttcaa tctttctctt ctcttcctct tcgctcatgg tgccagggcc ggggttctcc
     5461 tctacatctc cagcctgctt cagcagagag aaattcgtcg cgcctgatcc gtcgagtttc
     5521 actctcttag ctgcgggtga tcccccagat tccaattttt cgaatttctc caggatcttt
     5581 ttcttgaatg cttcccagat ctcttctttg tttttccatt tgccttccgc cttcagttcc
     5641 ttcttcgcct cttctacgat ttcctttgcc agcttggcca attcctcagt ttctttcttc
     5701 tctttctcct cctcctcttt tcgctttttt ttgaggtttt cccgcagtcg cctcataccc
     5761 tcctcgatct tccgcttttc ctcctcctcg gacatggggc ccgggttttc ttccacatct
     5821 ccgcatgtca ggaggctgcc tcttccctcg tccagtttca ccctctttgc tgctggcccg
     5881 ctaccaacct tgcgcttttt tttcggggac tcaaactcag agccatcggc agtcctcttt
     5941 gacccgccgc tctcattctt tttggcgaag tagtcatcct taaacaggat aagcagatca
     6001 gtctccttgt atttttgtcc tggggtttcc acaaatttct tagcgctttc tatggagtcg
     6061 aacacgacga atatggaacc tttaaaggcc ttgtgaaggg tgcggcgcat ctgaatgttc
     6121 aggacctgac ctttatcctc cagccactct ttaatatcgt caagggtggc gtctgttggg
     6181 aaccccttga tataaacgga cctatttttg acgtcgttct tatattcatc agtcacctca
     6241 ggcaagggtt tactaggtga tcttctaatc tttgtcttgt cctcagagat ctccataagt
     6301 tcggctttac tcttactcag ggcctccacg ataacattaa agtcggtcgt gagccggttc
     6361 aaccgattga acttaatcat tatttcgaga ggtacccagc cttcatccag ttttatctgt
     6421 tcctttagaa acttgtcccg tggcaaattg aagtcgccaa aataatactc aatttgatga
     6481 cagattttgg cctccagggc agccatcttt tcattatcac cattttcagc cattgacccc
     6541 ccgctgctgc ccccgctgct ttcaggtgtt gcgctctcgc ttgtgccagg tgtctcagag
     6601 ccagaggagc ctccgctaga tcctccggag tcacctccca gctgagacag gtcgatccgt
     6661 gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac ctctttggtg
     6721 ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc aggggctccc
     6781 agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat gggcttatcc
     6841 cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag gatcactctc
     6901 ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg ctgttccaca
     6961 aacagctgtt tctgctcatt atcctcgggg gagcccttca gcttctcata gtggctggcc
     7021 aggtacagga agttcacata tttggagggc agggccagtt cgtttccctt ctgcagttcg
     7081 ccggcagagg ccagcattct cttccggccg ttttccagct cgaacaggga gtacttaggc
     7141 agcttgatga tcaggtcctt tttcacttct ttgtagccct tggcttccag aaagtcgatg
     7201 ggattcttct cgaagctgct tctttccatg atggtgatcc ccagcagctc tttcacactc
     7261 ttcagtttct tggacttgcc cttttccact ttggccacca ccagcacaga ataggccacg
     7321 gtggggctgt cgaagccgcc gtacttctta gggtcccagt ccttctttct ggcgatcagc
     7381 ttatcgctgt tcctcttggg caggatagac tctttgctga agccgcctgt ctgcacctcg
     7441 gtctttttca cgatattcac ttggggcatg ctcagcactt tccgcacggt ggcaaaatcc
     7501 cggcccttat cccacacgat ctccccggtt tcgccgtttg tctcgatcag aggccgcttc
     7561 cggatctcgc cgttggccag ggtaatctcg gtcttgaaaa agttcatgat gttgctgtag
     7621 aagaagtact tggcggtagc cttgccgatt tcctgctcgc tcttggcgat catcttccgc
     7681 acgtcgtaca ccttgtagtc gccgtacacg aactcgcttt ccagcttagg gtactttttg
     7741 atcagggcgg ttcccacgac ggcgttcagg taggcgtcgt gggcgtggtg gtagttgttg
     7801 atctcgcgca ctttgtaaaa ctggaaatcc ttccggaaat cggacaccag cttggacttc
     7861 agggtgatca ctttcacttc ccggatcagc ttgtcattct cgtcgtactt agtgttcatc
     7921 cgggagtcca ggatctgtgc cacgtgcttt gtgatctgcc gggtttccac cagctgtctc
     7981 ttgatgaagc cggccttatc cagttcgctc aggccgcctc tctcggcctt ggtcagattg
     8041 tcgaactttc tctgggtaat cagcttggcg ttcagcagct gccgccagta gttcttcatc
     8101 ttcttcacga cctcttcgga gggcacgttg tcgctcttgc cccggttctt gtcgcttctg
     8161 gtcagcacct tgttgtcgat ggagtcgtcc ttcagaaagc tctgaggcac gatagcgtcc
     8221 acatcgtagt cggacagccg gttgatgtcc agttcctggt ccacgtacat atcccgccca
     8281 ttctgcaggt agtacaggta cagcttctcg ttctgcagct gggtgttttc cacggggtgt
     8341 tctttcagga tctggctgcc cagctctttg atgccctctt cgatccgctt cattctctcg
     8401 cggctgttct tctgtccctt ctgggtggtc tggttctctc tggccatttc gatcacgatg
     8461 ttctcgggct tgtgccggcc catcactttc acgagctcgt ccaccacctt cactgtctgc
     8521 aggatgccct tcttaatggc ggggctgccg gccagattgg caatgtgctc gtgcaggcta
     8581 tcgccctggc cggacacctg ggctttctgg atgtcctctt taaaggtcag gctgtcgtcg
     8641 tggatcagct gcatgaagtt tctgttggcg aagccgtcgg acttcaggaa atccaggatt
     8701 gtcttgccgg actgcttgtc ccggatgccg ttgatcagct tccggctcag cctgccccag
     8761 ccggtgtatc tccgccgctt cagctgcttc atcactttgt cgtcgaacag gtgggcatag
     8821 gttttcagcc gttcctcgat catctctctg tcctcaaaca gtgtcagggt cagcacgata
     8881 tcttccagaa tgtcctcgtt ttcctcattg tccaggaagt ccttgtcctt gataattttc
     8941 agcagatcgt ggtatgtgcc cagggaggcg ttgaaccgat cttccacgcc ggagatttcc
     9001 acggagtcga agcactcgat tttcttgaag tagtcctctt tcagctgctt cacggtcact
     9061 ttccggttgg tcttgaacag caggtccacg atggcctttt tctgctcgcc gctcaggaag
     9121 gcgggctttc tcattccctc ggtcacgtat ttcactttgg tcagctcgtt atacacggtg
     9181 aagtactcgt acagcaggct gtgcttgggc agcaccttct cgttgggcag gttcttatcg
     9241 aagttggtca tccgctcgat gaagctctgg gcggaagcgc ccttgtccac cacttcctcg
     9301 aagttccagg gggtgatggt ttcctcgctc tttctggtca tccaggcgaa tctgctgttt
     9361 cccctggcca gagggcccac gtagtagggg atgcggaagg tcaggatctt ctcgatcttt
     9421 tcccggttgt ccttcaggaa tgggtaaaaa tcttcctgcc gccgcagaat ggcgtgcagc
     9481 tctcccaggt ggatctggtg ggggatgctg ccgttgtcga aggtccgctg cttccgcagc
     9541 aggtcctctc tcttcagctt cacgagcagt tcctcggtgc cgtccatctt ttccaggatg
     9601 ggcttgatga acttgtagaa ctcttcctgg ctggctccgc cgtcaatgta gccggcgtag
     9661 ccgttcttgc tctggtcgaa gaaaatctct ttgtacttct caggcagctg ctgccgcacg
     9721 agagctttca gcagggtcag gtcctggtgg tgctcgtcgt atctcttgat catagaggcg
     9781 ctcagggggg ccttggtgat ctcggtgttc actctcagga tgtcgctcag caggatggcg
     9841 tcggacaggt tcttggcggc cagaaacagg tcggcgtact ggtcgccgat ctgggccagc
     9901 aggttgtcca ggtcgtcgtc gtaggtgtcc ttgctcagct gcagtttggc atcctcggcc
     9961 aggtcgaagt tgctcttgaa gttgggggtc
//
