LOCUS       sequence-492327-        9452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rep/Cap plasmid for producing variant AAV-hCA4-IV68.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Lin C, Chen X, Hoang JD, Ristic F, Fan Y, Jang S, Chung JHA, 
            Sullivan EE, Gawda T, Kavvathas B, Tran I, Li Y, Steele AD, Shay TF,
            Gradinaru V
  TITLE     AAVs targeting human carbonic anhydrase IV enhance gene delivery to 
            the brain.
  JOURNAL   Cell Rep. 2025 Oct 31;44(11):116419. doi: 
            10.1016/j.celrep.2025.116419.
  PUBMED    41175373
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2025-10-31"; volume: "44(11):116419. doi"; pages: " 
            10.1016/j.celrep.2025.116419"
COMMENT     Sequence Label: pUCmini-iCAP-hCA4-IV68
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2577..2687
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2726..2744)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2727..2744)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2764..2781)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2770..2994
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3056..3074
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3093..3111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3130..3148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3167..3185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3204..3222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3241..3259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3278..3296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3315..3333
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3352..3370
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3389..3407
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6124..6135
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6587..6607)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6587..6605)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6626..6642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6626..6642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6639..6661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6650..6666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6674..6680,6681..6698,6699..6704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6719..6740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6857..6874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7028..7616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7108..7127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7787..8578,8579..8647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8410..8429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8648..8752)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8778..9233)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8915..8936)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9127..9146
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9360..9382
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9374..9391
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9375..9391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9401..9420
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9401..9419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     join(9448..9452,1..12)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     join(9449..9452,1..13)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacggta tcgggggagc tcgcagggtc tccattttga agcgggaggt ttgaacgcgc
       61 agccgccatg ccggggtttt acgagattgt gattaaggtc cccagcgacc ttgacgagca
      121 tctgcccggc atttctgaca gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc
      181 gccagattct gacatggatc tgaatctgat tgagcaggca cccctgaccg tggccgagaa
      241 gctgcagcgc gactttctga cggaatggcg ccgtgtgagt aaggccccgg aggctctttt
      301 ctttgtgcaa tttgagaagg gagagagcta cttccacatg cacgtgctcg tggaaaccac
      361 cggggtgaaa tccatggttt tgggacgttt cctgagtcag attcgcgaaa aactgattca
      421 gagaatttac cgcgggatcg agccgacttt gccaaactgg ttcgcggtca caaagaccag
      481 aaatggcgcc ggaggcggga acaaggtggt ggatgagtgc tacatcccca attacttgct
      541 ccccaaaacc cagcctgagc tccagtgggc gtggactaat atggaacagt atttaagcgc
      601 ctgtttgaat ctcacggagc gtaaacggtt ggtggcgcag catctgacgc acgtgtcgca
      661 gacgcaggag cagaacaaag agaatcagaa tcccaattct gatgcgccgg tgatcagatc
      721 aaaaacttca gccaggtaca tggagctggt cgggtggctc gtggacaagg ggattacctc
      781 ggagaagcag tggatccagg aggaccaggc ctcatacatc tccttcaatg cggcctccaa
      841 ctcgcggtcc caaatcaagg ctgccttgga caatgcggga aagattatga gcctgactaa
      901 aaccgccccc gactacctgg tgggccagca gcccgtggag gacatttcca gcaatcggat
      961 ttataaaatt ttggaactaa acgggtacga tccccaatat gcggcttccg tctttctggg
     1021 atgggccacg aaaaagttcg gcaagaggaa caccatctgg ctgtttgggc ctgcaactac
     1081 cgggaagacc aacatcgcgg aggccatagc ccacactgtg cccttctacg ggtgcgtaaa
     1141 ctggaccaat gagaactttc ccttcaacga ctgtgtcgac aagatggtga tctggtggga
     1201 ggaggggaag atgaccgcca aggtcgtgga gtcggccaaa gccattctcg gaggaagcaa
     1261 ggtgcgcgtg gaccagaaat gcaagtcctc ggcccagata gacccgactc ccgtgatcgt
     1321 cacctccaac accaacatgt gcgccgtgat tgacgggaac tcaacgacct tcgaacacca
     1381 gcagccgttg caagaccgga tgttcaaatt tgaactcacc cgccgtctgg atcatgactt
     1441 tgggaaggtc accaagcagg aagtcaaaga ctttttccgg tgggcaaagg atcacgtggt
     1501 tgaggtggag catgaattct acgtcaaaaa gggtggagcc aagaaaagac ccgcccccag
     1561 tgacgcagat ataagtgagc ccaaacgggt gcgcgagtca gttgcgcagc catcgacgtc
     1621 agacgcggaa gcttcgatca actacgcgga caggtaccaa aacaaatgtt ctcgtcacgt
     1681 gggcatgaat ctgatgctgt ttccctgcag acaatgcgag agactgaatc agaattcaaa
     1741 tatctgcttc actcacggtg tcaaagactg tttagagtgc tttcccgtgt cagaatctca
     1801 acccgtttct gtcgtcaaaa aggcgtatca gaaactgtgc tacattcatc acatcatggg
     1861 aaaggtgcca gacgcttgca ctgcttgcga cctggtcaat gtggacttgg atgactgtgt
     1921 ttctgaacaa taaatgactt aaaccaggta tgagtcggct ggataaatct aaagtcataa
     1981 acggcgctct ggaattactc aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg
     2041 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     2101 tgctcgatgc cctggccatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     2161 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc
     2221 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     2281 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     2341 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     2401 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     2461 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     2521 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg
     2581 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     2641 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaaatgc
     2701 atgaattcga tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     2761 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2821 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2881 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2941 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     3001 aggcggaaag aaccagctgg ggctcgaatc aagctatcaa gtgccacctg acgtctccct
     3061 atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc
     3121 tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat
     3181 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
     3241 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
     3301 gacacgtctc gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca
     3361 gtgatagaga agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat
     3421 ataagcagag agatctgttc aaatttgaac tgactaagcg gctcccgcca gattttggca
     3481 agattactaa gcaggaagtc aaggactttt ttgcttgggc aaaggtcaat caggtgccgg
     3541 tgactcacga gtttaaagtt cccagggaat tggcgggaac taaaggggcg gagaaatctc
     3601 taaaacgccc actgggtgac gtcaccaata ctagctataa aagtctggag aagcgggcca
     3661 ggctctcatt tgttcccgag acgcctcgca gttcagacgt gactgttgat cccgctcctc
     3721 tgcgaccgct agcttcgatc aactacgcgg acaggtacca aaacaaatgt tctcgtcacg
     3781 tgggcatgaa tctgatgctg tttccctgca gacaatgcga gagactgaat cagaattcaa
     3841 atatctgctt cactcacggt gtcaaagact gtttagagtg ctttcccgtg tcagaatctc
     3901 aacccgtttc tgtcgtcaaa aaggcgtatc agaaactgtg ctacattcat cacatcatgg
     3961 gaaaggtgcc agacgcttgc actgcttgcg acctggtcaa tgtggacttg gatgactgtg
     4021 tttttgaaca ataaatgact taagccaggt atggctgccg atggttatct tccagattgg
     4081 ctcgaggaca accttagtga aggaattcgc gagtggtggg ctttgaaacc tggagcccct
     4141 caacccaagg caaatcaaca acatcaagac aacgctcgag gtcttgtgct tccgggttac
     4201 aaataccttg gacccggcaa cggactcgac aagggggagc cggtcaacgc agcagacgcg
     4261 gcggccctcg agcacgacaa ggcctacgac cagcagctca aggccggaga caacccgtac
     4321 ctcaagtaca accacgccga cgccgagttc caggagcggc tcaaagaaga tacgtctttt
     4381 gggggcaacc tcgggcgagc agtcttccag gccaaaaaga ggcttcttga acctcttggt
     4441 ctggttgagg aagcggctaa gacggctcct ggaaagaaga ggcctgtaga gcagtctcct
     4501 caggaaccgg actcctccgc gggtattggc aaatcgggtg cacagcccgc taaaaagaga
     4561 ctcaatttcg gtcagactgg cgacacagag tcagtcccag accctcaacc aatcggagaa
     4621 cctcccgcag ccccctcagg tgtgggatct cttacaatgg cttcaggtgg tggcgcacca
     4681 gtggcagaca ataacgaagg tgccgatgga gtgggtagtt cctcgggaaa ttggcattgc
     4741 gattcccaat ggctggggga cagagtcatc accaccagca cccgaacctg ggccctgccc
     4801 acctacaaca atcacctcta caagcaaatc tccaacagca catctggagg atcttcaaat
     4861 gacaacgcct acttcggcta cagcaccccc tgggggtatt ttgacttcaa cagattccac
     4921 tgccacttct caccacgtga ctggcagcga ctcatcaaca acaactgggg attccggcct
     4981 aagcgactca acttcaagct cttcaacatt caggtcaaag aggttacgga caacaatgga
     5041 gtcaagacca tcgccaataa ccttaccagc acggtccagg tcttcacgga ctcagactat
     5101 cagctcccgt acgtgctcgg gtcggctcac gagggctgcc tcccgccgtt cccagcggac
     5161 gttttcatga ttcctcagta cgggtatctg acgcttaatg atggaagcca ggccgtgggt
     5221 cgttcgtcct tttactgcct ggaatatttc ccgtcgcaaa tgctaagaac gggtaacaac
     5281 ttccagttca gctacgagtt tgagaacgta cctttccata gcagctacgc tcacagccaa
     5341 agcctggacc gactaatgaa tccactcatc gaccaatact tgtactatct ctcaaagact
     5401 attaacggtt ctggacagaa tcaacaaacg ctaaaattca gtgtggccgg acccagcaac
     5461 atggctgtcc agggaagaaa ctacatacct ggacccagct accgacaaca acgtgtctca
     5521 accactgtga ctcaaaacaa caacagcgaa tttgcttggc ctggagcttc ttcttgggct
     5581 ctcaatggac gtaatagctt gatgaatcct ggacctgcta tggccagcca caaagaagga
     5641 gaggaccgtt tctttccttt gtctggatct ttaatttttg gcaaacaagg aactggaaga
     5701 gacaacgtgg atgcggacaa agtcatgata accaacgaag aagaaattaa aactactaac
     5761 ccggtagcaa cggagtccta tggacaagtg gccacaaacc accagagtgc ccaagtttcg
     5821 ggtacgcttg cttatgcaca ggcgcagacc ggctgggttc aaaaccaagg aatacttccg
     5881 ggtatggttt ggcaggacag agatgtgtac ctgcaaggac ccatttgggc caaaattcct
     5941 cacacggacg gcaactttca cccttctccg ctgatgggag ggtttggaat gaagcacccg
     6001 cctcctcaga tcctcatcaa aaacacacct gtacctgcgg atcctccaac ggccttcaac
     6061 aaggacaagc tgaactcttt catcacccag tattctactg gccaagtcag cgtggagatc
     6121 gagtgggagc tgcagaagga aaacagcaag cgctggaacc cggagatcca gtacacttcc
     6181 aactattaca agtctaataa tgttgaattt gctgttaata ctgaaggtgt atatagtgaa
     6241 ccccgcccca ttggcaccag atacctgact cgtaatctgt aattgcttgt taatcaataa
     6301 accgtttaat tcgtttcagt tgaactttgg tctctgcgaa gggcgaattc gtttaaacct
     6361 gcaggactag aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc
     6421 atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg
     6481 gaggtttgaa cgcgcagccg ccaagccgaa ttctgcagat atccatcaca ctggcggccg
     6541 ctcgactaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     6601 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7801 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7861 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7921 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7981 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8041 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8101 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8161 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8221 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8281 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8341 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8401 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8461 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8521 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8581 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8641 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8701 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8761 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8821 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8881 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8941 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     9001 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     9061 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     9121 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     9181 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     9241 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     9301 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     9361 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     9421 cgaattgggt accgggcccc ccctcgatcg ag
//
