LOCUS       sequence-492361-       11734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified plasmid from pADH99 to perform HIS-FLP CRISPR in 
            Candidozyma auris.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Sofras D, Carolus H, Subotic A, Romero CL, Ennis CL, Hernday AD, 
            Nobile CJ, Rybak JM, Van Dijck P
  TITLE     A comparative evaluation of CRISPR-Cas9 allele editing systems in 
            Candida auris: challenging research in a challenging bug.
  JOURNAL   bioRxiv [Preprint]. 2025 Jan 11:2025.01.09.632232. doi: 
            10.1101/2025.01.09.632232.
  PUBMED    39829791
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jan 11:2025.01.09.632232. doi: 
            10.1101/2025.01.09.632232."
COMMENT     Sequence Label: pADH99Cau
FEATURES             Location/Qualifiers
     source          1..11734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(67..84)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(238..826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(318..337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(997..1788,1789..1857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1620..1639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1858..1962)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2030..2048
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    complement(3535..3568)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             3946..8049
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3946..8049
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8062..8082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8086..8106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8110..8130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8131..8196
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      8261..8508
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(8277..8295)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             9068..10339
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     promoter        10767..11110
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(join(11618..11624,11625..11642,11643..11648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11663..11684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
       61 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      121 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      181 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      241 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      301 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      361 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      421 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      481 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      541 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      601 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      661 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      721 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      781 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      841 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
      901 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
      961 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttga tcagtgaggc
     1021 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1081 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1141 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1201 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1261 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1321 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1381 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1441 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     1501 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     1561 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     1621 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     1681 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     1741 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     1801 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     1861 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     1921 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     1981 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2041 cacgaggccc tttgacggca cggccacgcg tttaaaccgc ctcaagcgga tttgcagctg
     2101 gtaaatcttt cgagaagcac aatattgtat actacaaatt tcttttccac actgcagact
     2161 atctcccttc gcaacataaa agtcacacac acacacacat catacgtatc atttttatac
     2221 ctactcacga aaactaatat catcgtggaa tcccctgagc atgtctgagt ggatctctac
     2281 ttttcctggt tacactctgg cagtgtccag agtgaaaaaa attgtgggaa gcatcataag
     2341 aactggccct actcctcgcc atgttggtgt catcatggac ggtaacagaa ggtatgcaaa
     2401 aagtcacaaa atcgagttga aagaaggaca taatttgggg ttcgactcga tggccaacat
     2461 tctcgagtta ctctatgagt caggtgttga gtgtgccact gtctacgcat tttcaattga
     2521 gaatttcaaa agactgcgct acgaggtcga gtggttgatg gacttagcca aatccaaatt
     2581 ccagcaaata agtcaacaag gcgaactttg cgaaaagtat ggtatcaaga taaaaatctt
     2641 gggcaacgtt gctctattgc cgtctgatgt acaggcaatc cttgccgaga cagaaaggat
     2701 tacgaagaac aacacaagag ctgtgctcaa tatttgtttt ccttacactt ctagagatga
     2761 gatggccacg tctatgagaa gaattgtgat gctatcggaa aaggtcccat cgctttttat
     2821 cgatgaatct accattgagg actacttgta cactgcagac tcaccacctc ttgatatctt
     2881 ggttcgtacc agtggaactt acaggctatc ggacttcttg ttgtggcagg cagtgcccgc
     2941 ttcttgttcg atcatgttca gcaacaagtt gtggccagat ttcactgcat gggacatgat
     3001 caagattctt ctcacctgga gcttccacgt gtactggtat ggaaatggta atggcattac
     3061 tggtgggcca acttctaagc ccattcatcc tgcaaagtct atagaaaaag atctctctcc
     3121 cctgccgggc ttgtcttcat cgacgacagc ttatgagagg tttcaggata attcaactga
     3181 tagtgagaag gattcactag gcaaatcgga cgtggaggat gttacatcta atctgtccac
     3241 gacatcagat gtcgcaaagt agcgatacac tagcatacta ctataccaag acactttgca
     3301 tcaacacaca agaatctagc tcggacttat ataccctcac tgagacaatt accatggact
     3361 tcttagatta cacaatcctc ctgatgaaga ggaattttct ctaatcgcat caactctatc
     3421 aaagacctca agatagtgta agtttaatat tcctgcagat gttggggtat tttaggttga
     3481 ctcattacta cacgcagccc tgaaaaatgg ttatcgagta tcgctaaatc tggggaagtt
     3541 cctatacttt ctagagaata ggaacttccc aggatctacg cgcattgcaa ctccgaggcc
     3601 gatacccttt gaccagtggt caattcgtca caattggcaa cattttcatg atttggcagc
     3661 ttcacgttga acaattgaac cagcagaatc cagcaaaacg agtttccgtt gtgaatgccc
     3721 ctttgtgtgt gcaaataatc atggtggctc cctaatggca taccctcagt cacctggagg
     3781 aattgctcgc tcgagtctcc tgaggtgtcc tcctgcctgt ggaaaaagcg gccaggtgtg
     3841 gtccaccgtt ggaacagctt gctgtataaa taccacaggt gcgcacgccc caggggcctt
     3901 tttttttttc tcctccctat ccaaacttaa ttttcatccc cggggatgga taaaaagtat
     3961 agtattggtt tagatattgg tactaactct gtgggttggg cagttatcac tgacgaatat
     4021 aaagttccat caaagaaatt taaggtgtta ggtaacactg acagacactc aataaaaaag
     4081 aatcttatcg gtgctctttt gttcgactcc ggtgaaactg ccgaggctac acgtttaaaa
     4141 agaacagcaa gaagaagata tacccgtaga aaaaatagaa tatgttattt acaagaaatc
     4201 ttttctaatg aaatggctaa agttgatgat tcctttttcc atagattgga agagtcattt
     4261 ttggttgaag aagacaaaaa gcatgagaga catccaatct ttgggaatat agttgatgaa
     4321 gtggcttacc atgaaaaata tcctaccatt tatcatttaa gaaagaaatt ggtagattca
     4381 actgataaag ctgaccttag attaatctat ttagcacttg cccatatgat taaatttaga
     4441 ggtcattttt tgattgaagg tgatttgaac ccagataatt ctgacgtgga taaattattt
     4501 attcaattag tccaaaccta caaccaatta tttgaggaaa atccaattaa tgctagtggt
     4561 gtcgatgcca aagctatatt atcagccaga ttatcaaaat ctagacgttt ggaaaatttg
     4621 attgcccaat tgccaggaga aaaaaagaat ggattatttg gaaacttgat cgcattatca
     4681 ttgggtttga caccaaattt taaatctaat tttgatttag ctgaagatgc taaattacaa
     4741 ttatcaaaag acacctatga cgacgatttg gacaatttac ttgctcaaat tggtgatcaa
     4801 tatgcagatt tgttcttagc tgctaaaaac ttatctgatg ctattttgtt gtctgatatt
     4861 ttgagagtga acacagaaat aaccaaagct ccattatcag catctatgat caaacgttat
     4921 gatgaacacc atcaggattt gactttattg aaagctttgg tgagacaaca attgccagag
     4981 aagtataaag aaatcttttt cgatcaatct aaaaacgggt atgcaggtta tattgatggg
     5041 ggtgcctccc aagaggaatt ttacaaattt ataaaaccta ttttagaaaa gatggatggg
     5101 actgaggaac ttttggtcaa attgaacaga gaagatttgt tacgtaaaca gagaactttt
     5161 gataatggta gtatacctca ccaaattcat ttgggtgagt tgcatgcaat tttaagaaga
     5221 caagaagatt tttatccatt tttaaaagat aatagagaaa aaatcgagaa aattttaacc
     5281 tttagaattc catactatgt tgggcctttg gctagaggta attcaagatt tgcctggatg
     5341 acacgtaaat cagaagaaac tattacccct tggaattttg aagaggttgt tgataaagga
     5401 gcatcagcac agagttttat tgaaagaatg accaatttcg ataaaaactt accaaatgaa
     5461 aaagttttac caaaacattc cttgttatac gaatatttta ctgtttacaa tgaacttaca
     5521 aaggttaaat atgttactga aggtatgcgt aagccagcct ttttatctgg agaacagaaa
     5581 aaggcaatag ttgatttatt gtttaaaaca aatagaaaag ttactgttaa acaattaaaa
     5641 gaagattact ttaagaaaat tgaatgtttt gattcagttg aaatcagtgg tgttgaagac
     5701 agatttaatg ctagtttagg aacttaccat gatttactta aaattatcaa agataaagat
     5761 ttcttggata acgaagaaaa tgaagacatt ttagaagaca ttgttttaac cttaacttta
     5821 ttcgaagata gagagatgat tgaagaacgt ttgaagactt atgcacattt gtttgacgat
     5881 aaagtgatga aacagttgaa aagaagacgt tatactggat ggggtagatt gtctcgtaaa
     5941 ttgatcaatg gaattagaga taaacaaagt ggtaaaacta tcttggactt tttgaaatct
     6001 gacggatttg ctaatagaaa tttcatgcaa ttgatccacg acgatagttt gacatttaaa
     6061 gaagacatcc aaaaggccca agtgagtggg caaggtgatt cattacatga acatattgca
     6121 aatttagccg gatctcctgc tattaagaaa gggatattac aaactgttaa agttgtggat
     6181 gaattagtga aagtaatggg aagacataaa cctgaaaaca ttgtcattga gatggcaaga
     6241 gaaaatcaaa ctacacaaaa aggacagaaa aatagtagag aacgtatgaa aagaatagaa
     6301 gagggtatta aagaattggg tagtcaaata ttgaaagaac acccagtgga aaatacccag
     6361 ttgcaaaatg aaaaattata tctttactac cttcaaaatg gacgtgatat gtatgttgat
     6421 caggaattag atataaatag actttcagat tatgatgtag atcatatagt tccacaatct
     6481 ttcttgaaag atgattccat agacaataaa gtattaacta gaagtgataa aaatagaggt
     6541 aaaagtgata atgtcccaag tgaggaagtc gtcaaaaaga tgaaaaatta ctggcgtcaa
     6601 cttttgaatg ctaaattaat tactcaaaga aaatttgata atttgactaa agcagaaaga
     6661 ggtgggcttt ctgaattaga taaagccggg ttcattaaaa gacaattggt cgaaactaga
     6721 caaattacta aacatgttgc ccaaatttta gattcccgta tgaacactaa gtatgacgaa
     6781 aatgataagt taatacgtga ggttaaagtc attactttaa aatcaaaact tgtctctgat
     6841 ttcagaaagg atttccaatt ctataaagtt agagaaatta ataattatca tcatgctcat
     6901 gatgcatatt tgaatgctgt agttggaact gctttaatca agaaataccc taaattagaa
     6961 tctgaatttg tatatggtga ttacaaagtc tatgatgtta gaaagatgat tgctaaatca
     7021 gaacaagaaa ttggtaaagc tacagctaaa tacttctttt actctaacat tatgaatttc
     7081 tttaaaacag aaattacttt ggcaaacggt gaaattagaa aaagacctct tattgaaaca
     7141 aatggtgaga ctggagagat agtttgggac aaagggcgtg atttcgctac tgttagaaaa
     7201 gttttatcaa tgccacaagt taacattgta aagaaaacag aggttcaaac tggtggtttc
     7261 tcaaaagaaa gtattttgcc taaaagaaat agtgataaat tgattgccag aaaaaaggat
     7321 tgggatccaa agaaatatgg tggtttcgac tcaccaaccg tagcctattc tgttttggtt
     7381 gtggcaaagg ttgaaaaggg taaaagtaaa aagcttaaat cagtaaaaga acttttgggt
     7441 attacaataa tggaaagaag ttcctttgaa aagaacccta ttgatttttt ggaagctaaa
     7501 ggttataagg aagtaaagaa ggacttaata atcaaattgc ctaaatattc tttatttgaa
     7561 ttagaaaatg ggagaaaaag aatgttggct tctgctggag aattgcaaaa gggtaatgaa
     7621 ttagcattgc cttccaaata tgttaacttc ttgtatttag cttcacacta tgaaaagttg
     7681 aaagggtcac cagaagataa cgagcaaaaa caattatttg ttgaacaaca caaacactac
     7741 ttagatgaga ttatagaaca aattagtgaa ttcagtaaaa gagtgatatt agctgatgca
     7801 aatttagata aagttttgtc agcctataac aaacatagag ataagccaat tagagaacaa
     7861 gcagaaaaca ttattcactt atttaccctt accaatttag gagcacctgc tgctttcaag
     7921 tattttgata caacaattga tcgtaaaaga tatacctcaa caaaagaagt cttagacgcc
     7981 accttaattc atcaatcaat cactggattg tatgagacaa gaattgattt gtctcaattg
     8041 ggtggtgatg aaggggctga tcctaagaag aaaagaaaag ttgatccaaa gaaaaagcgt
     8101 aaggtggatc ctaagaaaaa gagaaaggtt gactacaaag accatgacgg tgattataaa
     8161 gatcatgaca tcgactacaa ggatgacgat gacaagtgat aatgactgca gagatccatc
     8221 gacctgccgc caagctaatt ccgggcgaat ttctgtcgag tcatgtaatt agttatgtca
     8281 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
     8341 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
     8401 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
     8461 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg ccccaataat
     8521 gattggtttg atatttttgt ctagtaccat ctgtaccatt acacttaaat tatctttata
     8581 tctgtctaac tcgactgtct ggatttcatt gatgtagtcg tatgcatcgt tagttccaaa
     8641 aaatattgtc atcaatttga tattggtttc cgactctaaa atttttggaa gaatttgtct
     8701 agcgtgctct gagttgtagc cactgaaacc acggttaata acatccaatt ttcggatata
     8761 cacattctgt aatgctggat gaaagccata ctgggtacaa ctaaactggg tgatggagtc
     8821 accgaacaac acaaatttac cgtattccat gattgctatg gttgagaatt tttttttttt
     8881 tcttgtccca cgccattttt caaattatgc agttgagaat gttagttttt gtgtacaccc
     8941 cgttcgctga atatttcgga ataattcaaa gattggggag tgggggaggc gatagacgaa
     9001 gacacggtat aaaaatgggc aaaattttcc ccaacttttt gcagtggttt aactaataat
     9061 cgtcgacatg ccacaatttg atatattatg taaaacacca cctaaggtgc ttgttcgtca
     9121 gtttgtggaa aggtttgaaa gaccttcagg tgagaaaata gcattatgtg ctgctgaact
     9181 aacctattta tgttggatga ttacacataa cggaacagca atcaagagag ccacattcat
     9241 gagctataat actatcataa gcaattcgtt gagtttcgat attgtcaata aatcactcca
     9301 gtttaaatac aagacgcaaa aagcaacaat tttggaagcc tcattaaaga aattgattcc
     9361 tgcttgggaa tttacaatta ttccttacta tggacaaaaa catcaatctg atatcactga
     9421 tattgtaagt agtttgcaat tacagttcga atcatcggaa gaagcagata agggaaatag
     9481 ccacagtaaa aaaatgctta aagcacttct aagtgagggt gaaagcatct gggagatcac
     9541 tgagaaaata ctaaattcgt ttgagtatac ttcgagattt acaaaaacaa aaactttata
     9601 ccaattcctc ttcctagcta ctttcatcaa ttgtggaaga ttcagcgata ttaagaacgt
     9661 tgatccgaaa tcatttaaat tagtccaaaa taagtatttg ggagtaataa tccagtgttt
     9721 agtgacagag acaaagacaa gcgttagtag gcacatatac ttctttagcg caaggggtag
     9781 gatcgatcca cttgtatatt tggatgaatt tttgaggaat tctgaaccag tcctaaaacg
     9841 agtaaatagg accggcaatt cttcaagcaa taaacaggaa taccaattat taaaagataa
     9901 cttagtcaga tcgtacaata aagctttgaa gaaaaatgcg ccttattcaa tctttgctat
     9961 aaaaaatggc ccaaaatctc acattggaag acatttgatg acctcatttc tttcaatgaa
    10021 gggcctaacg gagttgacta atgttgtggg aaattggagc gataagcgtg cttctgccgt
    10081 ggccaggaca acgtatactc atcagataac agcaatacct gatcactact tcgcactagt
    10141 ttctcggtac tatgcatatg atccaatatc aaaggaaatg atagcattga aggatgagac
    10201 taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg aaggaagcat
    10261 acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc tttcatccta
    10321 cataaataga cgcatataag agtgaaattc tggaaatctg gaaatctggt tttgtattct
    10381 tgttattctt ctttttgtta ttacatatat aacttgttac ttttttaaaa aaatctttgt
    10441 atattttata aatatataaa actaaattta agaaaaagag aaaaatgttt tatttgagag
    10501 attgaaattt tacttgaatt tagcttagct tttataaagt attattatgt aaaaaaacaa
    10561 aacaaatata cattaaaaag ttaagactat aaaatagcca cccaaggcat ttctatatct
    10621 tgttgttgtt gttttcatct tctgtatcag aggaacttat tttattattt tcgtcacggg
    10681 tattttctct tgtttgatga ttcatcccat tcattccatc ataaaatatc aagcttgcct
    10741 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
    10801 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
    10861 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
    10921 acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
    10981 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
    11041 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
    11101 ataaacaacc ggatcctaca attatgtcta ctactttgga tgatactgct tatagataca
    11161 gaacttctgt tccaggtgat gctgaagcta ttgaagcttt ggatggttct ttcactaccg
    11221 atactgtttt tagagttact gctactggtg atggtttcac tttgagagaa gttccagttg
    11281 atccaccatt gactaaggtt ttcccagatg atgaatccga tgatgaatcc gatgctggtg
    11341 aagatggtga tccagattct agaactttcg ttgcttatgg tgatgatggt gatttggctg
    11401 gtttcgttgt tgtttcttat tctggttgga acagaagatt gactgttgaa gatattgaag
    11461 ttgctccaga acatagaggt catggtgttg gtagagcttt gatgggtttg gctactgaat
    11521 tcgccagaga aagaggtgct ggtcatttgt ggttggaagt taccaatgtt aatcggtgtt
    11581 taaaccccag cgcctggcgg gcgctcacaa ttccacacaa catacgagcc ggaagcataa
    11641 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    11701 tgcccgcttt ccagtcggga aacctgtcgt gcca
//
