LOCUS       sequence-49237-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AASDHPPT
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatgggcag cagccatcat catcatcatc accacagcag cggcagagaa aacttgtatt
       61 tccagggcca tatggagggc gtgcgctggg ccttttcctg cggcacttgg ctgccgagcc
      121 gagccgaatg gctgctggca gtgcgatcga ttcagcccga ggagaaggag cgcattggcc
      181 agttcgtctt tgcccgggac gctaaggcag ccatggctgg tcgtctgatg ataaggaaat
      241 tagttgcaga gaaattgaat atcccttgga atcatattcg tttgcaaaga actgcaaaag
      301 gaaaaccagt tcttgcaaag gactcatcga atccttaccc gaatttcaac tttaacatct
      361 ctcatcaagg agactatgca gtgcttgctg ctgaacctga gctgcaagtt ggaattgata
      421 taatgaagac tagttttcca ggtcgtggtt caattccaga attctttcat attatgaaaa
      481 gaaagtttac caacaaagaa tgggaaacaa tcagaagctt taaggatgag tggactcagc
      541 tggatatgtt ttataggaat tgggcactta aggaaagctt cataaaagcc attggtgttg
      601 gactaggatt tgaattgcag cggcttgaat ttgatctatc tccattaaac ttggatatag
      661 gccaagttta taaagaaaca cgtttattcc tggatggaga ggaagaaaaa gaatgggcat
      721 ttgaggaaag caaaatagat gagcaccatt ttgttgcagt tgctcttagg aaacccgatg
      781 gatctagaca tcaggatgtt ccatctcagg atgattccaa accaacccag aggcaattta
      841 ctattctcaa ctttaatgat ttaatgtcat ctgccgttcc catgacacct gaagatcctt
      901 cattttggga ctgtttttgc ttcacagaag aaattccaat acgaaatggt acaaagtcat
      961 gactcgagtc tggtaa
//
