LOCUS       sequence-492372-        5736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Construct for labelling microtubles in mammalian cells using 
            microtuble targeted mScarlet3-H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5736)
  TITLE     mScarlet3-H plasmids
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mScarlet3-H_MapTauC
FEATURES             Location/Qualifiers
     source          1..5736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      600..609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             606..1292
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     1004..1023
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2517..2638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2554..2573)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2608..2627
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2645..3100)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2782..2803)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2994..3013
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3127..3231
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3233..3590
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3233..3424
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3256..3276)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3441..3576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3625..4419
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3625..4419
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3679..3698)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4289..4308
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4607..4626)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4651..4698
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5027..5615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5516..5535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg
      601 ccaccatgga tagcaccgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg
      661 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      721 agggcaccca gaccgccaag ctgagggtga ccaagggtgg ccccctgccc ttctcctggg
      781 acatcctgtc ccctcagttc atgtacggct ccagggcctt cacgaagcac cccgccgaca
      841 tccccgacta ctggaagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      901 tcgaggacgg cggcgccgtg tccgtggccc aggacacctc cctggaggac ggcaccctga
      961 tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga
     1021 agacaatggg ctgggaagca tccaccgagc ggttgtaccc cgaggacgtc gtgctgaagg
     1081 gcgacattaa gcacgccctg cgcctgaagg acggcggccg ctacctggcg gacttcaaga
     1141 ccacctacag ggccaagaag cccgtgcaga tgcccggcgc cttcaacatc gaccgcaagt
     1201 tggacatcac atcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgtgg
     1261 cccgccactc caccggcggc tccggtggct ccttgtacaa gtccggaagc ggcgggtccg
     1321 ggagcggaag cgctgagccc cgccaggagt tcgaagtgat ggaagatcac gctgggacgt
     1381 acgggttggg ggacaggaaa gatcaggggg gctacaccat gcaccaagac caagagggtg
     1441 acacggacgc tggcctgaaa gctgaagaag caggcattgg agacaccccc agcctggaag
     1501 acgaagctgc tggtcacgtg acccaagctc gcatggtcag taaaagcaaa gacgggactg
     1561 gaagcgatga caaaaaagcc aagggggctg atggtaaaac gaagatcgcc acaccgcggg
     1621 gagcagcccc tccaggccag aagggccagg ccaacgccac caggattcca gcaaaaaccc
     1681 cgcccgctcc aaagacacca cccagctctg gtgaacctcc aaaatcaggg gatcgcagcg
     1741 gctacagcag ccccggctcc ccaggcactc ccggcagccg ctcccgcacc ccgtcccttc
     1801 caaccccacc cacccgggag cccaagaagg tggcagtggt ccgtactcca cccaagtcgc
     1861 cgtcttccgc caagagccgc ctgcagacag cccccgtgcc catgccagac ctgaagaatg
     1921 tcaagtccaa gatcggctcc actgagaacc tgaagcacca gccgggaggc gggaaggtgc
     1981 aaatagtcta caaaccagtt gacctgagca aggtgacctc caagtgtggc tcattaggca
     2041 acatccatca taaaccagga ggtggccagg tggaagtaaa atctgagaag cttgacttca
     2101 aggacagagt ccagtcgaag attgggtccc tggacaatat cacccacgtc cctggcggag
     2161 gaaataaaaa gattgaaacc cacaagctga ccttccgcga gaacgccaaa gccaagacag
     2221 accacggggc ggagatcgtg tacaagtcgc cagtggtgtc tggggacacg tctccacggc
     2281 atctcagcaa tgtctcctcc accggcagca tcgacatggt agactcgccc cagctcgcca
     2341 cgctagctga cgaggtgtct gcctccctgg ccaagcaggg tttgtgagga tccaccggat
     2401 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     2461 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     2521 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2581 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     2641 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2701 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     2761 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     2821 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     2881 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     2941 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3001 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3061 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     3121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     3241 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3301 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3421 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3481 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3541 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     3601 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3661 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3721 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3781 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     3841 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     3901 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     3961 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4021 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4081 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4141 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4201 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4261 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4321 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4381 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4441 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4501 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4561 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     4621 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4681 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     4741 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     4801 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     4861 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5161 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5701 ctgtggataa ccgtattacc gccatgcatt agttat
//
