LOCUS       sequence-492373-        4710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C1 Cloning vector for mammalian cells incorporating mScarlet3 -H red
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  TITLE     mScarlet3-H plasmids
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pmScarlet3H-C1
FEATURES             Location/Qualifiers
     source          1..4710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..99
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(398..417)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    442..489
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      818..1406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1307..1326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1581..1884
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1885..2088
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2039..2059
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2127..2136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2133..2819
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     2531..2550
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2829..2894
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    3018..3139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3055..3074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3109..3128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3146..3601)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3283..3304)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3495..3514
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3628..3732
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3734..4091
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3734..3925
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3757..3777)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3942..4077
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4126..4710,1..210)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(4126..4710,1..210)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4180..4199)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
       61 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
      121 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
      181 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
      241 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
      301 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
      361 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
      421 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
      481 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
      541 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
      601 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
      661 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      721 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      781 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      841 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      901 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      961 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1021 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1081 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1141 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1201 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1261 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1321 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1381 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1441 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1501 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
     1561 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1621 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     1681 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     1741 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     1801 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1861 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     1921 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     1981 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2041 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     2101 cgtcagatcc gctagcgcta ccggtcgcca ccatggatag caccgaggca gtgatcaagg
     2161 agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg
     2221 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg agggtgacca
     2281 agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca
     2341 gggccttcac gaagcacccc gccgacatcc ccgactactg gaagcagtcc ttccccgagg
     2401 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgccgtgtcc gtggcccagg
     2461 acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc
     2521 ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcatcc accgagcggt
     2581 tgtaccccga ggacgtcgtg ctgaagggcg acattaagca cgccctgcgc ctgaaggacg
     2641 gcggccgcta cctggcggac ttcaagacca cctacagggc caagaagccc gtgcagatgc
     2701 ccggcgcctt caacatcgac cgcaagttgg acatcacatc ccacaacgag gactacaccg
     2761 tggtggaaca gtacgaacgc tccgtggccc gccactccac cggcggctcc ggtggctcct
     2821 tgtacaagtc cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     2881 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     2941 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3001 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3061 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3121 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     3181 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     3241 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     3301 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     3361 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     3421 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     3481 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     3541 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     3601 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3661 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3721 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     3781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3841 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3901 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3961 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4021 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4081 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4141 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4201 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4261 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     4321 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4381 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4441 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4501 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4561 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4621 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     4681 gtgacccatg gcgatgcctg cttgccgaat
//
