LOCUS       sequence-49239-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH
  TITLE     Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor 
            neurons derived from human embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2008 Dec 4;3(6):649-57.
  PUBMED    19041781
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2008-12-4"; volume: "3(6)"; pages: "649-57"
COMMENT     Sequence Label: Lenti-Hb9-RFP
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(308..328)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(726..737)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aagggccgcc accaccctgt tcctgtagga attctgcaga tatccatcac actggcggcc
       61 gcgactctag atcataatca gtttaaactc tagaggatcc accggtggcc gcctgggccc
      121 gttaacgtct cgagtttaaa cctggaaaaa catggagcaa tcacaagtag caatacagca
      181 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
      241 gtcacacctc agacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
      301 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
      361 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
      421 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
      481 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
      541 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
      601 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
      661 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
      721 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
      781 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccc
//
