LOCUS       sequence-49240-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Kwon HJ, Palnitkar M, Deisenhofer J
  TITLE     The structure of the NPC1L1 N-terminal domain in a closed 
            conformation.
  JOURNAL   PLoS One. 2011 Apr 15;6(4):e18722.
  PUBMED    21525977
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011-04-15"; volume: "6(4)"; pages: "e18722"
COMMENT     Sequence Label: pfast NPC1L1(NTD)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     81..143
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             156..173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
       61 gcggatctcg gtccgaaacc atgaaattct tagtcaacgt tgcccttgtt tttatggtcg
      121 tatacatttc ttacatctat gcggattcgt actaccatca ccatcaccat cacgattacg
      181 atatcccaac gaccgaaaac ctgtattttc agggcgccga gccttacaca accatccacc
      241 agcctggcta ctgcgccttc tatgacgaat gtgggaagaa cccagagctg tctggaagcc
      301 tcatgacact ctccaacgtg tcctgcctgt ccaacacgcc ggcccgcaag atcacaggtg
      361 atcacctgat cctattacag aagatctgcc cccgcctcta caccggcccc aacacccaag
      421 cctgctgctc cgccaagcag ctggtatcac tggaagcgag tctgtcgatc accaaggccc
      481 tcctcacccg ctgcccagcc tgctctgaca attttgtgaa cctgcactgc cacaacacgt
      541 gcagccccaa tcagagcctc ttcatcaatg tgacccgcgt ggcccagcta ggggctggac
      601 aactcccagc tgtggtggcc tatgaggcct tctaccagca tagctttgcc gagcagagct
      661 atgactcctg cagccgtgtg cgcgtccctg cagctgccac gctggctgtg ggcaccatgt
      721 gtggcgtgta tggctctgcc ctttgcaatg cccagcgctg gctcaacttc cagggagaca
      781 caggcaatgg tctggcccca ctggacatca ccttccacct cttggagcct ggccaggccg
      841 tggggagtgg ga
//
