LOCUS       sequence-492403-        9943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  24-stem loop MS2 Citrine + magnetic separation RNA reporter, 
            stem-loops in 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Thurm AR, Finkel Y, Andrews C, Cai XS, Benko C, Bintu L
  TITLE     High-throughput discovery of regulatory effector domains in human 
            RNA-binding proteins.
  JOURNAL   bioRxiv [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317.
  PUBMED    39071298
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317."
COMMENT     Sequence Label: pAT122
FEATURES             Location/Qualifiers
     source          1..9943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    275..296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(311..316,317..334,335..341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    349..365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     354..376
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     373..389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     373..389
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        410..428
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    463..1266
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1273..1298
                     /label=SA
                     /note="splice acceptor site"
     CDS             1322..1375
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1385..1984
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1385..1404)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1881..1901
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2016..2033)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2022..2246
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2319..2337
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2355..2373
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2391..2409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2427..2445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2463..2481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2535..2553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2571..2589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2607..2625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          2870..3808
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3765..3785
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3841..3859
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(3869..3880)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      3890..3899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3896..3955
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             4727..4756
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4760..4906
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             4922..4975
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4982..4991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4988..4990,4991..4993,4994..5704)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5033..5054)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5294..5313)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5641..5662
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5705..5725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7062..7183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7099..7118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7153..7172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    7247..8083
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(8358..9149,9150..9218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8981..9000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9342..9930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9831..9850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
       61 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      121 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      181 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      241 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      301 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      361 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcaga attaaccctc
      421 actaaaggga ctagtcctgc aggtttaaac caattggccc tttgctttct ctgaccagca
      481 ttctctcccc tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac
      541 ggctggccca gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt
      601 ccccttgctc tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg
      661 gcgctgcacc acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc
      721 atctctcctc cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct
      781 ccttctgggg cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc
      841 cttgcgtccc gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc
      901 aaagtacccc gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga
      961 caccccgttc tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct
     1021 acccccctta cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca
     1081 ggggtccgag agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg
     1141 acagcatgtt tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc
     1201 cagggccggt taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag
     1261 tggggcaagc ttctgacctc ttctcttcct cccacagggc ctcgagagat caggcagcgg
     1321 agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct
     1381 cgagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     1441 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     1501 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1561 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1621 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1681 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1741 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1801 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     1861 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     1921 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     1981 ctgatctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     2041 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     2101 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     2161 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2221 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct gggggctagc
     2281 aaagcctagg aacagctgca tatagcactg cagtttactc cctatcagtg atagagagtc
     2341 aaaaggccat gccctcccta tcagtgatag agagagcaga ctcaaggtgg tccctatcag
     2401 tgatagagag attgagcaag agggcctccc tatcagtgat agagaatagc actgcagttt
     2461 actccctatc agtgatagag agtcaaaagg ccatgccctc cctatcagtg atagagagag
     2521 cagactcaag gtggtcccta tcagtgatag agagattgag caagagggcc tccctatcag
     2581 tgatagagat tgatccagtc agggcctccc tatcagtgat agagaagcta tgtgcaggta
     2641 gggctgtaca gtgggaatgc atgagcgcac atcgcccaca gtccccgaga agttgggggg
     2701 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     2761 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     2821 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     2881 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     2941 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3001 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     3061 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     3121 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     3181 agatagtctt gtaaatgcgg gccaacatgt gcacactggt atttcggttt ttggggccgc
     3241 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     3301 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     3361 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     3421 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     3481 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     3541 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     3601 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     3661 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     3721 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     3781 ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agcgattacg ccaagctcga
     3841 aattaaccct cactaaaggg aacaaaagct ggagctccca ccgcggtggg ccaccatgga
     3901 gacagacaca ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacgc
     3961 tagcgagacg ggcggatcag gcggtcgtct cggatccgac aaaactcaca catgcccacc
     4021 gtgcccagca cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa
     4081 ggacaccctc atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca
     4141 cgaagaccct gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa
     4201 gacaaagccg cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt
     4261 cctgcaccag gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct
     4321 cccagccccc atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt
     4381 ctacaccctg cccccatccc gggaggagat gaccaagaac caggtcagcc tgacctgcct
     4441 ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga
     4501 gaacaactac aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag
     4561 caagctcacc gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat
     4621 gcacgaggct ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaaagg
     4681 aggaggtgga agcggaggag gaggaagcgg aggaggaggt agcggagaac aaaaactcat
     4741 ctcagaagag gatctgaatg ctgtgggcca ggacacgcag gaggtcatcg tggtgccaca
     4801 ctccttgccc tttaaggtgg tggtgatctc agccatcctg gccctggtgg tgctcaccat
     4861 catctccctt atcatcctca tcatgctttg gcagaagaag ccacgttgta caggcagtgg
     4921 agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaccggt
     4981 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5041 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5101 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5161 gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct accccgacca
     5221 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5281 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5341 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5401 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     5461 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5521 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5581 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5641 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5701 caagccaaaa aagaagagaa aggtatagtt gcggccgcga attcgccctt cgcgggatcc
     5761 tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc gaatcttcaa
     5821 acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatccta cggtacttat
     5881 tgccaagaaa gcacgagcat cagccgtgcc tccaggtcga atcttcaaac gacgacgatc
     5941 acgcgtcgct ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc
     6001 acgagcatca gccgtgcctc caggtcgaat cttcaaacga cgacgatcac gcgtcgctcc
     6061 agtattccag ggttcatcag atcctacggt acttattgcc aagaaagcac gagcatcagc
     6121 cgtgcctcca ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag tattccaggg
     6181 ttcatcagat cctacggtac ttattgccaa gaaagcacga gcatcagccg tgcctccagg
     6241 tcgaatcttc aaacgacgac gatcacgcgt cgctccagta ttccagggtt catcagatcc
     6301 tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc gaatcttcaa
     6361 acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatccta cggtacttat
     6421 tgccaagaaa gcacgagcat cagccgtgcc tccaggtcga atcttcaaac gacgacgatc
     6481 acgcgtcgct ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc
     6541 acgagcatca gccgtgcctc caggtcgaat cttcaaacga cgacgatcac gcgtcgctcc
     6601 agtattccag ggttcatcag atcctacggt acttattgcc aagaaagcac gagcatcagc
     6661 cgtgcctcca ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag tattccaggg
     6721 ttcatcagat cctacggtac ttattgccaa gaaagcacga gcatcagccg tgcctccagg
     6781 tcgaatcttc aaacgacgac gatcacgcgt cgctccagta ttccagggtt catcagatcc
     6841 tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc gaatcttcaa
     6901 acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatccta cggtacttat
     6961 tgccaagaaa gcacgagcat cagccgtgcc tccaggtcga atcttcaaac gacgacgatc
     7021 acgcgtcgct ccagtattcc agggttcatc agatctaact aaacttgttt attgcagctt
     7081 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7141 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgcatgtaaa
     7201 ttgtaagcgt ctcggcctct agagtcgaga tgacattgat tattgaacta gggacaggat
     7261 tggtgacaga aaagccccat ccttaggcct cctccttcct agtctcctga tattgggtct
     7321 aacccccacc tcctgttagg cagattcctt atctggtgac acacccccat ttcctggagc
     7381 catctctctc cttgccagaa cctctaaggt ttgcttacga tggagccaga gaggatcctg
     7441 ggagggagag cttggcaggg ggtgggaggg aaggggggga tgcgtgacct gcccggttct
     7501 cagtggccac cctgcgctac cctctcccag aacctgagct gctctgacgc ggctgtctgg
     7561 tgcgtttcac tgatcctggt gctgcagctt ccttacactt cccaagagga gaagcagttt
     7621 ggaaaaacaa aatcagaata agttggtcct gagttctaac tttggctctt cacctttcta
     7681 gtccccaatt tatattgttc ctccgtgcgt cagttttacc tgtgagataa ggccagtagc
     7741 cagccccgtc ctggcagggc tgtggtgagg aggggggtgt ccgtgtggaa aactcccttt
     7801 gtgagaatgg tgcgtcctag gtgttcacca ggtcgtggcc gcctctactc cctttctctt
     7861 tctccatcct tctttcctta aagagtcccc agtgctatct gggacatatt cctccgccca
     7921 gagcagggtc ccgcttccct aaggccctgc tctgggcttc tgggtttgag tccttggcaa
     7981 gcccaggaga ggcgctcagg cttccctgtc ccccttcctc gtccaccatc tcatgcccct
     8041 ggctctcctg ccccttccct acaggggttc ctggctctgc tcttcgcctt cttgacgagt
     8101 tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     8161 gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8221 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc aaaaaggatc
     8281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8881 tccgtaagat gcttttctgt gactggtgaa tactcaacca agtcattctg agaatagtgt
     8941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9241 tgaagcattt atcagggtta ttgtctcatg accaaaatcc cttaacgtga gttttcgttc
     9301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acg
//
