LOCUS       sequence-492414-        8966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DOX-suppressible IKBKG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8966)
  TITLE     Targeting DNA polymerase epsilon leads to tumor clearance and 
            activation of an NF-_B-mediated inflammatory response in triple 
            negative breast cancer
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW57.1 DOX off puro IKBKG
FEATURES             Location/Qualifiers
     source          1..8966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             546..611
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        1908..2418
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2337..2355
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2428..3024
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2428..2447)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2924..2944
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3025..3078
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3152..3171)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             3706..3816
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     primer_bind     complement(3865..3885)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4283..4294)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4499..4732
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4804..4925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4841..4860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4895..4914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4965..5100
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5100..5171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5172..5240,5241..6032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5390..5409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6203..6791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6692..6711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6945..6962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7079..7100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7115..7120,7121..7138,7139..7145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7153..7169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7158..7180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7177..7193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7177..7193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7212..7232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7214..7232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7260..7486
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7487..7667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7714..7839
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8332..8565
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8750..8794
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggacta taaggatcac gatggtgact acaaggacca cgacatcgac tacaaggacg
      601 acgacgacaa gggaggcggt ggaggcatga ataggcacct ctggaagagc caactgtgtg
      661 agatggtaca gccgagcgga ggcccggcag cagatcagga cgtactgggc gaagagtctc
      721 ctctggggaa gccagccatg ctgcacctgc cttcagaaca gggcgctcct gagaccctcc
      781 agcgctgcct ggaagagaac caggaactgc gagatgccat ccggcagagc aaccagattc
      841 tgcgggagcg ctgcgaggag cttctgcatt tccaagccag ccagagggag gagaaggagt
      901 tcctcatgtg caagttccag gaggccagga aactggtgga gagactcggc ctggagaagc
      961 tcgatctgaa gaggcagaag gagcaggctc tgcgggaggt ggagcacctg aagagatgcc
     1021 agcagcagat ggctgaggac aaggcctctg tgaaagccca ggtgacgtcc ttgctcgggg
     1081 agctgcagga gagccagagt cgcttggagg ctgccactaa ggaatgccag gctctggaag
     1141 gtcgagctcg agcagcaagc gaacaagcac gacaactgga aagtgaacgt gaagcacttc
     1201 aacaacagca tagtgtacaa gtagatcaac tacgtatgca aggacaaagt gttgaagctg
     1261 cactacgtat ggaacgccag gccgcctcgg aggagaagag gaagctggcc cagttgcagg
     1321 tggcctatca ccagctcttc caagaatacg acaaccacat caagagcagc gtggtgggca
     1381 gtgagcggaa gcgaggaatg cagctggaag atctcaaaca gcagctccag caggccgagg
     1441 aggccctggt ggccaaacag gaggtgatcg ataagctgaa ggaggaggcc gagcagcaca
     1501 agattgtgat ggagaccgtt ccggtgctga aggcccaggc ggatatctac aaggcggact
     1561 tccaggctga gaggcaggcc cgggagaagc tggccgagaa gaaggagctc ctgcaggagc
     1621 agctggagca gctgcagagg gagtacagca aactgaaggc cagctgtcag gagtcggcca
     1681 ggatcgagga catgaggaag cgtcatgtag aagtatctca ggcccccttg cctcctgctc
     1741 ctgcttacct ctcctctccc ctggccctgc ccagccagag gaggagccct ccagaagaac
     1801 cacctgattt ctgctgtccc aagtgccagt atcaggcccc tgatatggac accctgcaga
     1861 tacatgtcat ggagtgcatt gagtagtagg tcgacgaatt cggatccggg gttggggttg
     1921 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     1981 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     2041 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     2101 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     2161 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     2221 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     2281 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     2341 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     2401 accgacctct ctccccagca attcaccatg accgagtaca agcccacggt gcgcctcgcc
     2461 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     2521 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     2581 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     2641 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     2701 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     2761 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     2821 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     2881 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     2941 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     3001 tgcatgaccc gcaagcccgg tgccgaaggt agaggttctc tcctcacttg tggtgatgtt
     3061 gaagaaaacc ctggtccaat gtctagatta gataaaagta aagtgattaa cagcgcatta
     3121 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
     3181 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
     3241 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
     3301 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
     3361 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
     3421 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
     3481 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
     3541 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
     3601 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
     3661 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat
     3721 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     3781 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatcga
     3841 tccaagatat cgtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3901 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3961 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4021 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4081 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4141 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4201 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4261 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4321 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4381 cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt acctttaaga
     4441 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4501 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4561 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4621 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4681 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     4741 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     4801 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4861 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4921 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     4981 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5041 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5101 tttcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5161 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5221 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5281 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5341 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5401 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5461 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5521 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5581 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5641 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5701 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5761 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5821 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5881 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5941 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6001 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6061 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6121 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6781 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6841 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6901 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6961 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7021 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7081 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7141 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7201 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7261 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7321 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7381 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7441 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     7501 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7561 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7621 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7681 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7741 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7801 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7861 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7921 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7981 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8041 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8101 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8161 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8221 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8281 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8341 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8401 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8461 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8521 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8581 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8641 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8701 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8761 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8821 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8881 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8941 gacccacctc ccaaccccga ggggac
//
