LOCUS       sequence-49242-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Lin MC, Galletta BJ, Sept D, Cooper JA
  TITLE     Overlapping and distinct functions for cofilin, coronin and Aip1 in 
            actin dynamics in vivo.
  JOURNAL   J Cell Sci. 2010 Apr 15;123(Pt 8):1329-42. Epub 2010 Mar 23.
  PUBMED    20332110
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-04-15"; volume: "123(Pt 8)"; pages: "1329-42. 
            Epub 2010 Mar 23"
COMMENT     Sequence Label: pBJ1808 pRS416-COF1RFP
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(118..573)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(255..276)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     467..486
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     699..721
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     713..730
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     714..730
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     740..759
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        740..758
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     783..799
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     784..800
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atagtaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
       61 taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
      121 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      181 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
      241 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
      301 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
      361 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      421 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      481 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      541 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
      601 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      661 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      721 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc
      781 cctcgaggtc gacggtatcg ataagcttga tatcga
//
