LOCUS       sequence-49243-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ448 - pDESTR4-R3(ttTi4348, I, unc-18)
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    114..135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(150..155,156..173,174..180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    188..204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     193..215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     212..228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     212..228
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtaatgtg
      121 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      181 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgggtcacca
      241 ggtatcgcgc tttaattagg gtgcaagttt tttataattt ttaatcccat taaaattcaa
      301 agcagaaagg ttctgatgct ctacgcaaaa tatcaaaatc acaatttatg acaatattgt
      361 tacaaaaatc caaaaaaaaa attgcagttc gaaatggtga ttgaagaaaa cggattacgt
      421 gaagctactc gacgaatcat atcctatttg gaaaaatata ttcatgcact gcactatcat
      481 cataaggatg atgagattta ataatttttg taatttatct tacaattagc attttattta
      541 attttaaaaa aatttgaatg ggaacataaa gtcaaattcc tgagctgcat ggctaagggt
      601 gccctcgaaa attt
//
