LOCUS       sequence-492504-       11922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HaloTag-RPA194.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Ide S, Imai R, Ochi H, Maeshima K
  TITLE     Transcriptional suppression of ribosomal DNA with phase separation.
  JOURNAL   Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 
            10.1126/sciadv.abb5953. Print 2020 Oct.
  PUBMED    33055158
REFERENCE   2  (bases 1 to 11922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-10-14"; volume: "6(42). pii: 6/42/eabb5953. doi"; pages:
            " 10.1126/sciadv.abb5953. Print 2020 Oct"
COMMENT     Sequence Label: pEF1-FRT-HaloTag-RPA194
FEATURES             Location/Qualifiers
     source          1..11922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5290..6174)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     regulatory      6180..6189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6263..6282)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6264..6282)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6299..7477)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6308..7246
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6331..6351)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7765..7784
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7859..7963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7964..8032,8033..8824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8182..8201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8995..9583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9484..9503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9737..9754
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9871..9892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9907..9912,9913..9930,9931..9937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9945..9961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9950..9972
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9969..9985
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9969..9985
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    10034..10155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10045..10064)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10099..10118
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10285..11307)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(10285..11307)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(11315..11362)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11494..11513
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    11646..11870
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     11859..11876
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(11914..11922,1..33))
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct ctagactcga
       61 gcggccgcca ctgtgctgga tctatctcag aggctgcttg agctcgaaca ggcctgtccc
      121 gcccctgacg accttcccga ccacaaggca ggcagaagga gacctcagct catcgtggga
      181 tcccagcatg gtggcttgct tcagaaactg gaagctggtt tcaaatgtca tctgctgtag
      241 cggggaagag tttgaccgga tcccaaagcg attcagtggc ttgtaaacac cctcgaagca
      301 catataatca gcaaccaggg agagatggcg agggtcgacc gcgatgccat acacggcaaa
      361 cacatccttg atctccttct cgatcacccg cagcgcggcc tcaatgccat acgtgttggc
      421 tatggcgtgg atgtcgttgg agtagaggcg gcgcagatcc aggacctctg catacttgaa
      481 tagctctggg aggttgattc cttctgtgtt tagcacaagc tccttctcgt tcttattgtt
      541 ggttgtttca ttcaggaggc accgagtgat gcccttggtc gcatagatga cggcaccatg
      601 ggccaaagat actaccaggg agctcatgtc aaagttgatc ttcatcagag ggagcttcac
      661 tgtcacctgg caccacaggc tctcctcggt gtcgtactgg tagtcatcta tgaacgggtg
      721 gatctcacgc acagcctgga cccggcgctc catggcctcg ggcccctggg gctcctggct
      781 gtgggtgggt ttccggggct gcgtcaggag ggcgggaagg gacgggtcct cctcagtgcc
      841 taagcccacc tcttcatctt gctcttgggt ctttcgagca ccttccctgt ggggatttcg
      901 ttcctcctgc atgtcttcat cgtcgttctc ctcgccctcc ctctcctcct cttcctcact
      961 ctcataatca acctcctcct cctgcttctc cttgcgtttg gcatcagagg catcggcgtc
     1021 tccctcctca gcttcagcat ccacaatgtg cccctcctct tcctcatcac cctcctgctc
     1081 tccccgactc ctccccaact ccccagcgtt gtccagatcc cgctgtgtag ctcttcgagt
     1141 gtttacgttc ctgaaagctg atgctttatt attcttcttt ttgatggatt ccatcagaag
     1201 tttaaagaat cttgtttcca tgaagcgcag gatgtcctcg ggtctcaggc acttctcctg
     1261 ctggtaatat gcatgtggca ggaactgaaa ccgcagctgg tacacctgga atttgttctg
     1321 tttttcttcc atacagaagg actcctggac gtcaattttc tgcaacacct cccccaagca
     1381 caccctggtg agttgcttct tcaggctttt cactctcttc agggctttct tggtgttgag
     1441 cacgggcacg ctcatcatgg gtgtcttgat gttggcgctg gccaccatga gaatctcccg
     1501 caaccttgga atgcccaggg tgacgttcat ctcgcctctg cctgcaaagt ggaaggtgtt
     1561 gagggtcatc tgggtggagg gctctccgat gctctgggca gccagcaggc ccacagcctc
     1621 gcccggctca cacagtgagc gctgccactt cagctgcagc aaggtcctca acctgtcgag
     1681 agaaagctct gatttctcat aactcttctc tgtttgagct gcccactctt gactgtagtc
     1741 atcaaccttt gtttcaaatg tttctgacac tgatgcaaag tagatgtcag gacgccagac
     1801 agacagactg gggtcaggac aagcggccgc cttcttctgg tatttccttc ggctttcctc
     1861 atccaactca taccacatcc tcagcatctc ctgagtccca gggctgcggc cattgcggtt
     1921 ttcactctca agtttcaggg ctttcacagc ttcctgaatt ttctgggaat aactcaagaa
     1981 ggcgcctctt ctcagcaggg tgttggggtg cttgctttgc cattttttga tagctctgaa
     2041 gtggtggaga gcttttttgg gatctgctct ggataaaact tcatggagat gctgtgattt
     2101 cattatcacc tcgtagttgc tggccaggaa ggggaactgc ttgggctgca ggaactgtgt
     2161 cttggggatg tccaggccat cctccccata caggaactgc accacactgc cgtcactgtc
     2221 acggaccgtg agatcatact gcacgaccag cccctctagg tgcttgatga tgcacctttg
     2281 gagatagcct gagcggctgg ttttcacagc agtgtccacc aggccctctc gtcctgccat
     2341 gcagtggaag aagaactcag gaggtttgat gccggtgagg aacctgccag tgacaaagcc
     2401 accagccctg ggggtgaact cataaggctc aaagcagggc agtgacttgc cagacgccat
     2461 cagcgggggt ctccgacctt ccagttcaat ctggcccagc aggcacgaga tctgcatcgt
     2521 gttcacagtt gaacctttgg ctcccgactg caccatcatc tgcaggctgt tctctgggaa
     2581 ctgtctgtgt aggccaaaag gcatgcatgc cttgttaatc tcattgctgt aatggttcac
     2641 ttcctccttg aacttcagat caatcatgtt aaaatccctc tggtccttgc ccagatgggc
     2701 atcctgccat tttcctcgga cctcatcata tgatgcggct tctggcaggt ttaatgcagc
     2761 cctgacagcc tggggcccgc agtgggtgga ttcttcaatg atacgttgcc tcttgacatc
     2821 tgcctttggc ttcaccaaaa tgtcttccac gcccaaggtg aagcctctgt agagctgcag
     2881 gtaggcggtg aagaggcggg ccaggcaggt tagaaccttg ccgctggtct cgcctccata
     2941 gatctcatag cagcagtgga ccaggccgta ggcggagctc ccatagtgcg ccttgtccag
     3001 cactccgcag agcagctccc cttccctgat gatcacctgg gactcgcaca tcgagtcagg
     3061 gttaaagcca ggaacggatc gaggagtttc cttcacccag gctttcccag tgattttcgc
     3121 ctttccagat aagttcagtg ggatgtggtc ctctgggatt atatttatga gcagcgttga
     3181 cacaacctgt tttcctgtcc acagcggaaa gggcttcagg atggaaggag aaaggagctt
     3241 cacgcgcccc actttgtccg tgagtcctcg gtacaccagc tccatatagt gctcccgggt
     3301 gaaaaagcaa ccccgagtag tcatgcttgc ccctgaaacc atgtgatcct ggatcagtcc
     3361 cgccaatggt tggccatcct tgggaacaag gtactgctga tcagtgcagg ccaggacgta
     3421 ggcctcggcc cggcccagct cactctgggg gaaatgggca ttcatctcgt ctccatcaaa
     3481 gtcggcatta taggccttgc agttggcata gtggagccgc agcactttct cttcaggcag
     3541 gatgcgggca cggtgggcct ggatggaggg tctgtgcagt gtgggctgtc ggttcagtag
     3601 cagaatgtcc ccattcttca catgccggca cacaattttt gtcccctggg gcttaggtgc
     3661 ccccgtggct ggggtcagaa gctgcttggc cacggcctct cgctgggtca tgtccacagc
     3721 gctcagggct gtgcggctgc cgtcctcatt gatgaccatg gaggctcctg ggtgcacatt
     3781 agggccgttg atgaccgctt gcctaagttc ctgaacattc catggggtaa ctggctgtgg
     3841 gtaggtcagt tttgtggcaa acaccatggg aattccaatt tcgttggtgt tgatgtacat
     3901 gtctgggcag atgactgagc gcgcagcgta gtccactcgc tttcccatca tgtgttttcg
     3961 gaacaggcct tctttcttct ccaggatctg cctaatgcct gggtacttgt ccatcattag
     4021 tttgtccatc tcgctatcaa acacaatatt gacgtggctc tgaaggcgaa tccaaatgtt
     4081 gtaaagtttg tctatgaggg actggcctgg aagtgtactc aaaaaggatc ggtcaatagc
     4141 aatcaaagag tctttttcct catctgtagt gggtgtggcc acttcctctg gcaacttctg
     4201 ttcttgggcc atcaatgcca gaagttttcg aatcagaact acatccttca tgacagcctg
     4261 caagttcacc gtctggccat tagtaaacat ctggtctcct aggcgactga ctgggcgata
     4321 ccttgagggc ggcaccacca agaaatctag aaagaacaca ctgggattga atctggattc
     4381 cataccatca tcatccattc ccgaaaaaag gtagttcaga aagaatcctt cattcttcca
     4441 cagggcagaa aggtgttcgc gggcactggt gggtgttaag tatcctcgtt ttcctatctg
     4501 agcttcctca attcccaggg gctcagagtc cttctggcca gctgtcctgt gcaccatggc
     4561 tggaaatgtg atagtcaact tgctgttgtg ttcctttcgg acaacggatc gcccggtctt
     4621 gcagtgggga cagcgcttag cattcatatg tgccttccag aagagagcaa tgagcttgct
     4681 cttgctctca cacacgttct ttacatgtgc gccctgggac cccaggaggt tgttctgcac
     4741 aatttcagtt gtgtattgtt ctaattcctc ccgaatttca gaggcagagg gatcggcatt
     4801 ttcttccaga aacctgttca gaattctctc aagctcgtag actgcttgta gggccccgac
     4861 ttccagaacc ctcagctggc agagtaagag gtgaatcacg gcccggggac aagtcagcat
     4921 gtggcagttt aaacaagagc cccgaagcag caggtacagc ttatcgaaga ggagagggtt
     4981 atacactgtg agtgggagct caatgtggcc caggtgccca gaacagttgc tgaagtcctg
     5041 cacgcaggtg gagcacacct ctttggaatc tgcagggccc aaagctaaat cgtacaggcc
     5101 gtttgccgat gggttcccca ggctgtccag gtatcgaggg ttcgtaatgg atttaacact
     5161 taatttcttg agctcttcag ccgaatacat cccgaaggaa atgccctgca gccgccgcca
     5221 gggcatgttc ttggagatca acattgaacc gccgccacca gatccacctc caccagatcc
     5281 acctccacca ccggaaatct ccagagtaga cagccagcgc gcgatctcgc tgccgatcag
     5341 gtccgggttg tcttcttgca gcagattcag acccgggccg atgtccacag ccttgcagtt
     5401 aggcaggctt ttggccaggc gagcggcttc ggccggtggg atcagaacgc ctggggtgcc
     5461 ccagaacagc agcttcggga caggggactg gtgcagccag tccatgtatt cttcgaccag
     5521 cgcgacgatg ttcgctggct caccggcgat tggcagctcg tttgggaagc gccacagtgg
     5581 ctcgcggtca acaggattca ggaacggctc gcggtaatgg tccatctcga cttcagtcag
     5641 cgggcggacg acacccatcg gcagcgtacc ctcgataaaa acgttctgat cgatgatcag
     5701 cttgcggccg acgtcggtgg tgcggaaggc ctggaaggtc tcgcgggcaa attctggcca
     5761 ttcgtcccag gtcgggatag ggcggatgaa ctccataaat gcaatacctt tgacgcgctc
     5821 tggattgcgc ttggcccagt ggaaacccag agcggagccc cagtcgtgaa tgaccaggac
     5881 gacctcttcc agacccaggg cttcgatgaa ggcatccatg aagcggacgt ggtcgtcgaa
     5941 gaaataaccc aggtctggtt tgtcggattt gcccataccg atcaggtctg gagcaatgca
     6001 gcgatgggtc ggtgcaacat gcgggatgat gttgcgccac acgtaggagg aggtcgggtt
     6061 accgtgcagg aacagcacag gggtgccatc gcgcggacca acatcgacgt agtgcatgcg
     6121 ctcgcccagg acttccacat aatgggggtc gaatggaaag ccagtaccga tttcggatcc
     6181 catggtggca tctgcagaat tccaccacac tggactagtg gatccgagct cggtaccaag
     6241 cttacctagc cagcttgggt ctccctatag tgagtcgtat tagtaccaag ctaattcctc
     6301 acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc
     6361 aagatccaaa ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc
     6421 taacttcagt ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc
     6481 aacctaaaga cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc
     6541 ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg
     6601 tgggtgactc acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag
     6661 cagggccggg aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg
     6721 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag
     6781 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac
     6841 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag
     6901 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag
     6961 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt
     7021 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag
     7081 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct
     7141 cgggatcaag aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat
     7201 aacccgtaaa gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg
     7261 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
     7321 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
     7381 accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg
     7441 gcgatgtgcg ctctgcccac tgacgggcac cggagccaat tcccactcct ttcaagacct
     7501 agaaggtcca ttagctgcaa agattcctct ctgtttaaaa ctttatccat ctttgcaaag
     7561 ctttttgcaa aagcctagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc
     7621 gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt
     7681 gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag
     7741 ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7801 ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg
     7861 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7921 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7981 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8041 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8101 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8161 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8221 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8281 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8341 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8401 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8461 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8521 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8581 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8641 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8701 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8761 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8821 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8881 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8941 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9001 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9061 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9121 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9181 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9241 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9421 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9541 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9601 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9661 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9721 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9781 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9841 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9901 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     9961 atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg
    10021 tatacagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
    10081 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
    10141 tgcaataaac aagttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
    10201 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
    10261 ggaatcgaaa tctcgtagta cgtgctattc ctttgccctc ggacgagtgc tggggcgtcg
    10321 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
    10381 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
    10441 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
    10501 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
    10561 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
    10621 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
    10681 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
    10741 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
    10801 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
    10861 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
    10921 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
    10981 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
    11041 gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc
    11101 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
    11161 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
    11221 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
    11281 agacgtcgcg gtgagttcag gctttttggc caaggaagtt cctatacttt ctagagaata
    11341 ggaacttcgg aataggaact tctaggtacg tgaaccatca ccctaatcaa gttttttggg
    11401 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
    11461 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
    11521 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
    11581 tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc
    11641 agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
    11701 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
    11761 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
    11821 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga
    11881 tcagcgggtt taaactcatt actaaccggt acgcgtagaa tc
//
