LOCUS       sequence-492509-       11922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HaloTag-RPA194-E593Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Ide S, Imai R, Ochi H, Maeshima K
  TITLE     Transcriptional suppression of ribosomal DNA with phase separation.
  JOURNAL   Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 
            10.1126/sciadv.abb5953. Print 2020 Oct.
  PUBMED    33055158
REFERENCE   2  (bases 1 to 11922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-10-14"; volume: "6(42). pii: 6/42/eabb5953. doi"; pages:
            " 10.1126/sciadv.abb5953. Print 2020 Oct"
COMMENT     Sequence Label: pEF1-FRT-HaloTag-RPA194-E593Q
FEATURES             Location/Qualifiers
     source          1..11922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..52
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     101..120
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        101..119
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      194..203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             209..1093
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             6350..6391
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6429..6446)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6435..6659
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6792..6811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6943..6990
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6998..8020
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6998..8020
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8150..8271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8187..8206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8241..8260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8320..8336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8320..8336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8333..8355)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8344..8360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8368..8374,8375..8392,8393..8398))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8413..8434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8551..8568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8722..9310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8802..8821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9481..10272,10273..10341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10104..10123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10342..10446)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10521..10540)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(10828..11922,1..84)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(11059..11922,1..75)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
       61 tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactataggg
      121 agacccaagc tggctaggta agcttggtac cgagctcgga tccactagtc cagtgtggtg
      181 gaattctgca gatgccacca tgggatccga aatcggtact ggctttccat tcgaccccca
      241 ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac
      301 ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc
      361 gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga
      421 caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga
      481 agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt
      541 ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg
      601 ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg
      661 caccaccgac gtcggccgca agctgatcat cgatcagaac gtttttatcg agggtacgct
      721 gccgatgggt gtcgtccgcc cgctgactga agtcgagatg gaccattacc gcgagccgtt
      781 cctgaatcct gttgaccgcg agccactgtg gcgcttccca aacgagctgc caatcgccgg
      841 tgagccagcg aacatcgtcg cgctggtcga agaatacatg gactggctgc accagtcccc
      901 tgtcccgaag ctgctgttct ggggcacccc aggcgttctg atcccaccgg ccgaagccgc
      961 tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg gtctgaatct
     1021 gctgcaagaa gacaacccgg acctgatcgg cagcgagatc gcgcgctggc tgtctactct
     1081 ggagatttcc ggtggtggag gtggatctgg tggaggtgga tctggtggcg gcggttcaat
     1141 gttgatctcc aagaacatgc cctggcggcg gctgcagggc atttccttcg ggatgtattc
     1201 ggctgaagag ctcaagaaat taagtgttaa atccattacg aaccctcgat acctggacag
     1261 cctggggaac ccatcggcaa acggcctgta cgatttagct ttgggccctg cagattccaa
     1321 agaggtgtgc tccacctgcg tgcaggactt cagcaactgt tctgggcacc tgggccacat
     1381 tgagctccca ctcacagtgt ataaccctct cctcttcgat aagctgtacc tgctgcttcg
     1441 gggctcttgt ttaaactgcc acatgctgac ttgtccccgg gccgtgattc acctcttact
     1501 ctgccagctg agggttctgg aagtcggggc cctacaagca gtctacgagc ttgagagaat
     1561 tctgaacagg tttctggaag aaaatgccga tccctctgcc tctgaaattc gggaggaatt
     1621 agaacaatac acaactgaaa ttgtgcagaa caacctcctg gggtcccagg gcgcacatgt
     1681 aaagaacgtg tgtgagagca agagcaagct cattgctctc ttctggaagg cacatatgaa
     1741 tgctaagcgc tgtccccact gcaagaccgg gcgatccgtt gtccgaaagg aacacaacag
     1801 caagttgact atcacatttc cagccatggt gcacaggaca gctggccaga aggactctga
     1861 gcccctggga attgaggaag ctcagatagg aaaacgagga tacttaacac ccaccagtgc
     1921 ccgcgaacac ctttctgccc tgtggaagaa tgaaggattc tttctgaatt accttttttc
     1981 gggaatggat gatgatggta tggaatccag attcaatccc agtgtgttct ttctagattt
     2041 cttggtggtg ccgccctcaa ggtatcgccc agtcagtcgc ctaggagacc agatgtttac
     2101 taatggccag acggtgaact tgcaggctgt catgaaggat gtagttctga ttcgaaaact
     2161 tctggcattg atggcccaag aacagaagtt gccagaggaa gtggccacac ccactacaga
     2221 tgaggaaaaa gactctttga ttgctattga ccgatccttt ttgagtacac ttccaggcca
     2281 gtccctcata gacaaacttt acaacatttg gattcgcctt cagagccacg tcaatattgt
     2341 gtttgatagc gagatggaca aactaatgat ggacaagtac ccaggcatta ggcagatcct
     2401 ggagaagaaa gaaggcctgt tccgaaaaca catgatggga aagcgagtgg actacgctgc
     2461 gcgctcagtc atctgcccag acatgtacat caacaccaac gaaattggaa ttcccatggt
     2521 gtttgccaca aaactgacct acccacagcc agttacccca tggaatgttc aggaacttag
     2581 gcaagcggtc atcaacggcc ctaatgtgca cccaggagcc tccatggtca tcaatgagga
     2641 cggcagccgc acagccctga gcgctgtgga catgacccag cgagaggccg tggccaagca
     2701 gcttctgacc ccagccacgg gggcacctaa gccccagggg acaaaaattg tgtgccggca
     2761 tgtgaagaat ggggacattc tgctactgaa ccgacagccc acactgcaca gaccctccat
     2821 ccaggcccac cgtgcccgca tcctgcctga agagaaagtg ctgcggctcc actatgccaa
     2881 ctgcaaggcc tataatgccg actttgatgg agaccagatg aatgcccatt tcccccagag
     2941 tgagctgggc cgggccgagg cctacgtcct ggcctgcact gatcagcagt accttgttcc
     3001 caaggatggc caaccattgg cgggactgat ccaggatcac atggtttcag gggcaagcat
     3061 gactactcgg ggttgctttt tcacccggga gcactatatg gagctggtgt accgaggact
     3121 cacggacaaa gtggggcgcg tgaagctcct ttctccttcc atcctgaagc cctttccgct
     3181 gtggacagga aaacaggttg tgtcaacgct gctcataaat ataatcccag aggaccacat
     3241 cccactgaac ttatctggaa aggcgaaaat cactgggaaa gcctgggtga aggaaactcc
     3301 tcgatccgtt cctggcttta accctgactc gatgtgcgag tcccaggtga tcatcaggga
     3361 aggggagctg ctctgcggag tgctggacaa ggcgcactat gggagctccg cctacggcct
     3421 ggtccactgc tgctatgaga tctatggagg cgagaccagc ggcaaggttc taacctgcct
     3481 ggcccgcctc ttcaccgcct acctgcagct ctacagaggc ttcaccttgg gcgtggaaga
     3541 cattttggtg aagccaaagg cagatgtcaa gaggcaacgt atcattgaag aatccaccca
     3601 ctgcgggccc caggctgtca gggctgcatt aaacctgcca gaagccgcat catatgatga
     3661 ggtccgagga aaatggcagg atgcccatct gggcaaggac cagagggatt ttaacatgat
     3721 tgatctgaag ttcaaggagg aagtgaacca ttacagcaat gagattaaca aggcatgcat
     3781 gccttttggc ctacacagac agttcccaga gaacagcctg cagatgatgg tgcagtcggg
     3841 agccaaaggt tcaactgtga acacgatgca gatctcgtgc ctgctgggcc agattgaact
     3901 ggaaggtcgg agacccccgc tgatggcgtc tggcaagtca ctgccctgct ttgagcctta
     3961 tgagttcacc cccagggctg gtggctttgt cactggcagg ttcctcaccg gcatcaaacc
     4021 tcctgagttc ttcttccact gcatggcagg acgagagggc ctggtggaca ctgctgtgaa
     4081 aaccagccgc tcaggctatc tccaaaggtg catcatcaag cacctagagg ggctggtcgt
     4141 gcagtatgat ctcacggtcc gtgacagtga cggcagtgtg gtgcagttcc tgtatgggga
     4201 ggatggcctg gacatcccca agacacagtt cctgcagccc aagcagttcc ccttcctggc
     4261 cagcaactac gaggtgataa tgaaatcaca gcatctccat gaagttttat ccagagcaga
     4321 tcccaaaaaa gctctccacc acttcagagc tatcaaaaaa tggcaaagca agcaccccaa
     4381 caccctgctg agaagaggcg ccttcttgag ttattcccag aaaattcagg aagctgtgaa
     4441 agccctgaaa cttgagagtg aaaaccgcaa tggccgcagc cctgggactc aggagatgct
     4501 gaggatgtgg tatgagttgg atgaggaaag ccgaaggaaa taccagaaga aggcggccgc
     4561 ttgtcctgac cccagtctgt ctgtctggcg tcctgacatc tactttgcat cagtgtcaga
     4621 aacatttgaa acaaaggttg atgactacag tcaagagtgg gcagctcaaa cagagaagag
     4681 ttatgagaaa tcagagcttt ctctcgacag gttgaggacc ttgctgcagc tgaagtggca
     4741 gcgctcactg tgtgagccgg gcgaggctgt gggcctgctg gctgcccaga gcatcggaga
     4801 gccctccacc cagatgaccc tcaacacctt ccactttgca ggcagaggcg agatgaacgt
     4861 caccctgggc attccaaggt tgcgggagat tctcatggtg gccagcgcca acatcaagac
     4921 acccatgatg agcgtgcccg tgctcaacac caagaaagcc ctgaagagag tgaaaagcct
     4981 gaagaagcaa ctcaccaggg tgtgcttggg ggaggtgttg cagaaaattg acgtccagga
     5041 gtccttctgt atggaagaaa aacagaacaa attccaggtg taccagctgc ggtttcagtt
     5101 cctgccacat gcatattacc agcaggagaa gtgcctgaga cccgaggaca tcctgcgctt
     5161 catggaaaca agattcttta aacttctgat ggaatccatc aaaaagaaga ataataaagc
     5221 atcagctttc aggaacgtaa acactcgaag agctacacag cgggatctgg acaacgctgg
     5281 ggagttgggg aggagtcggg gagagcagga gggtgatgag gaagaggagg ggcacattgt
     5341 ggatgctgaa gctgaggagg gagacgccga tgcctctgat gccaaacgca aggagaagca
     5401 ggaggaggag gttgattatg agagtgagga agaggaggag agggagggcg aggagaacga
     5461 cgatgaagac atgcaggagg aacgaaatcc ccacagggaa ggtgctcgaa agacccaaga
     5521 gcaagatgaa gaggtgggct taggcactga ggaggacccg tcccttcccg ccctcctgac
     5581 gcagccccgg aaacccaccc acagccagga gccccagggg cccgaggcca tggagcgccg
     5641 ggtccaggct gtgcgtgaga tccacccgtt catagatgac taccagtacg acaccgagga
     5701 gagcctgtgg tgccaggtga cagtgaagct ccctctgatg aagatcaact ttgacatgag
     5761 ctccctggta gtatctttgg cccatggtgc cgtcatctat gcgaccaagg gcatcactcg
     5821 gtgcctcctg aatgaaacaa ccaacaataa gaacgagaag gagcttgtgc taaacacaga
     5881 aggaatcaac ctcccagagc tattcaagta tgcagaggtc ctggatctgc gccgcctcta
     5941 ctccaacgac atccacgcca tagccaacac gtatggcatt gaggccgcgc tgcgggtgat
     6001 cgagaaggag atcaaggatg tgtttgccgt gtatggcatc gcggtcgacc ctcgccatct
     6061 ctccctggtt gctgattata tgtgcttcga gggtgtttac aagccactga atcgctttgg
     6121 gatccggtca aactcttccc cgctacagca gatgacattt gaaaccagct tccagtttct
     6181 gaagcaagcc accatgctgg gatcccacga tgagctgagg tctccttctg cctgccttgt
     6241 ggtcgggaag gtcgtcaggg gcgggacagg cctgttcgag ctcaagcagc ctctgagata
     6301 gatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     6361 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttaaacccg
     6421 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     6481 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     6541 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     6601 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6661 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     6721 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6781 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6841 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6901 cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag
     6961 ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac
     7021 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     7081 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     7141 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     7201 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     7261 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     7321 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     7381 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     7441 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     7501 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     7561 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     7621 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     7681 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     7741 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     7801 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     7861 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     7921 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     7981 aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga
     8041 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     8101 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     8161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8281 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8341 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8401 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8461 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8521 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8581 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8641 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8701 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8761 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8821 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8881 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8941 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9001 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9061 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9121 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9181 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9241 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9301 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9361 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9421 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9481 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9541 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9601 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9661 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9721 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9781 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9841 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9901 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9961 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10021 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10081 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10141 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10201 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10261 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10321 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10381 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10441 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    10501 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    10561 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    10621 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    10681 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag gcttttgcaa
    10741 aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
    10801 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
    10861 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
    10921 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
    10981 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
    11041 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
    11101 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
    11161 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
    11221 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
    11281 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
    11341 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
    11401 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
    11461 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
    11521 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
    11581 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
    11641 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
    11701 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
    11761 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
    11821 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
    11881 gttaggccag cttggcactt gatgtaattc tccttggaat tt
//
