LOCUS       sequence-492510-        8920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLP/FRT based mammalian expression vector for mouse histone 
            H3.1-EGFP, driven by mouse histone H3.1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Imai R, Nozaki T, Tani T, Kaizu K, Hibino K, Ide S, Tamura S, 
            Takahashi K, Shribak M, Maeshima K
  TITLE     Density imaging of heterochromatin in live cells using 
            orientation-independent-DIC microscopy.
  JOURNAL   Mol Biol Cell. 2017 Nov 7;28(23):3349-3359. doi: 
            10.1091/mbc.E17-06-0359. Epub 2017 Aug 23.
  PUBMED    28835378
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-06-0359"; journalName: "Mol Biol Cell"; date: 
            "2017-11-7- 7"; volume: "28"; issue: "23"; pages: "3349-3359"
COMMENT     Sequence Label: pIx2-mH3.1p-H3.1-EGFP-3′UTR-FRT
FEATURES             Location/Qualifiers
     source          1..8920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2597..2701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2702..2770,2771..3562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2920..2939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3733..4321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4222..4241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4475..4492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4609..4630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4645..4650,4651..4668,4669..4675)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4683..4699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4688..4710
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4707..4723
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4707..4723
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4772..4893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4783..4802)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4837..4856
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5023..6045)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(5023..6045)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(6053..6100)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(join(6716..7429,7430..7432,7433..7435))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6719..7429,7430..7432,7433..7435))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6761..6782)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7110..7129
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7369..7390
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     join(8916..8920,1..15)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatcggcc gcttgcaaag
       61 cttgcatgcc tgcaggtcga gaatattctc actgattccg tcctggagtt ggatgagaga
      121 taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat ttaccttctc
      181 caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca ccgtccctgg
      241 aggtgatgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg cacggtgggt
      301 gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga tttgacgatt
      361 gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg acccagctgt
      421 atctctccag gcaagctctt ccaccccttc tgcttgcatc cagacaccat caaacatgca
      481 ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc catgctgggt
      541 tttaacttgc tctttgtcct tctatcctat cttcttatcc ttaaggctgt tctgaacgct
      601 gtgacttgga gagtgtccca gagccctcaa cacctgcatg tcccacgtcc atgctgtcct
      661 gcacttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca aacatgtgtc
      721 accccagacc aacaatgtca cagcaaactc ccccttgata ggacaagggg gaatggcttt
      781 acactgagac aggggaggtt tgggttggat atgaggaggc agtttttccc ccagagggtg
      841 gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg caggcattca
      901 aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttgatgacc ctgcacatag
      961 cagggggttg gatctggatg agcactgtgc tcctttgcaa cccaggccgt tctatgattc
     1021 tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg
     1081 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     1141 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     1201 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     1261 gggctttggg ggggggctgt ccccgtgagc tgtcgagaat attctcactg attccgtcct
     1321 ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg ctggatttag
     1381 caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac ggaggtgggg
     1441 gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag ggacatggcc
     1501 agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct tttccagcct
     1561 tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc tgtcccagtc
     1621 ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct tgcatccaga
     1681 caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc tttttgtgct
     1741 cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc ttatccttaa
     1801 ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc tgcatgtccc
     1861 acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg atgcactgaa
     1921 ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc ttgataggac
     1981 aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga ggaggcagtt
     2041 tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt ggatgcccca
     2101 tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg ctgctggttg
     2161 atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct ttgcaaccca
     2221 ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
     2281 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     2341 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     2401 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     2461 gtaattacat ccctgggggc tttggggggg ggctgtcccc gtgagctgtc gactctagag
     2521 aataggaact tcggaatagg aacgcggccg atctcccgat ccgtcgacgt caggtggcac
     2581 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2641 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2701 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2761 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2821 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2881 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2941 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3001 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3061 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3121 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3181 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3241 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3301 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3361 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3421 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3481 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3541 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3601 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3661 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3721 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3781 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3841 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3901 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3961 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4021 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4081 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4141 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4201 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4261 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4321 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4381 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4441 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4501 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     4561 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     4621 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     4681 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     4741 ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc
     4801 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4861 tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag
     4921 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     4981 ttcatagaag gcggcggtgg aatcgaaatc tcgtagtacg tgctattcct ttgccctcgg
     5041 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     5101 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     5161 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     5221 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc
     5281 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     5341 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     5401 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     5461 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     5521 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     5581 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     5641 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
     5701 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     5761 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac
     5821 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     5881 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     5941 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     6001 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttggcca aggaagttcc
     6061 tatactttct agagaatagg aacttcggaa taggaacttc taggtacgtg aaccatcacc
     6121 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6181 cccccgattt agagcttgac ggggaaagaa gctgctggtt gtagcacttt gggttgttct
     6241 gggctatacg tcttccctat ggttttcttc atgtttctcc acccatccaa ccatttaacc
     6301 aggggacttc tggtatacaa aactaactca ggttctcttg ggactggata gctgagcagt
     6361 taaaatgctt ccagcaattc catgtataag acataggaaa ccatgaattt aaatgcctat
     6421 aattgactcc gccggcctta tattgctggc aaaataatta aaaatccttc aagaaaccga
     6481 acagtaagtg catttgggga ataattagag ctaatgttca gcttaagtat attggagttt
     6541 gcagaatact caccaactta gacactctaa agagcatccc tcagctaaaa aaaactgtta
     6601 gaacctggct accgttccaa gaaaaaaact ccaattggcc tataagtcaa cagcaccttt
     6661 gtgtaggttt gggtggctct gaaaagagcc tttgaaaaac acagaaagac gaactttact
     6721 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     6781 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     6841 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     6901 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     6961 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     7021 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     7081 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     7141 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     7201 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     7261 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     7321 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     7381 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accattggat
     7441 ccgagctcgg taccaagcta gccctctccc cgcggatgcg gcgggccagc tggatgtcct
     7501 tgggcatgat ggtgacacgc ttggcgtgga tggcgcacag gttggtgtcc tcaaacagac
     7561 ccacgaggta ggcctcacag gcctcctgca gagccatgac ggccgagctc tggaagcgca
     7621 ggtcggtctt gaagtcctgc gcgatctcgc gcaccaggcg ctggaacggc agcttgcgga
     7681 tcagcagctc ggtcgacttc tggtagcgcc ggatctcgcg cagcgccacg gtgccgggac
     7741 ggtagcggtg aggcttcttc acgccgccgg tggccggggc gctcttgcgg gcggccttgg
     7801 tggccagctg cttgcgcggg gccttgccgc cggtggactt gcgagcggtc tgcttagtac
     7861 gagccatggt aacacccgag cttccaaagc aaagtgatgg agggaaagct tcaaccacca
     7921 aaatttatac agacggccga gttctgattg gtcaaaatgg ctgccagatc aacttaattg
     7981 gctggtgagt acttttgttt tcgcagtctg atcatgtgtt taaccaagtg tgcaatttcc
     8041 tcattgaaaa gaaaatcccg ccgcaggttc gtttaaatca atgtaatttt tattaaaagc
     8101 acttttctta ctttcttttt cctacttcag attaaatgaa aagatgtata aaaggtaggt
     8161 agtgacttta aaaagaggct ctaagaacat tgacccccct ggaattcaat ttgccgaacg
     8221 aggtgtgtgt gtagggggga gggtgtctag gggaaatgtt tttagctgtg ataccaccaa
     8281 atgttttaac atcctttgct gattttgact ttgccaaatg gcaggacttg tcaatcatgc
     8341 acataaacag gattcaagtg aggattgatt gtacagtgat tagttgttta tcagcacatg
     8401 tgctgataaa ggaacatttc aagacaattc tttgtgcagt ccggtctgtc ctggaactcg
     8461 tcctgcagag taggctggcc tggagttcaa gatgtgtctg ttttatgtac taggtccgag
     8521 gttgggtgcc tgccttccaa aaaccaatat actcttagct aaggttaggg gttgaactaa
     8581 ccacaagcca gggaaaacta ggagaggtaa tacacgtatt tcccacaatg cactatccca
     8641 gtagtgttta ggtctctttg gagaaagtga gtggctctga aaagagcctt tggtgattcg
     8701 taatgtttaa gctttttgca aaagcctagt caataatcaa tgtcaacgcg tatatctggc
     8761 ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc
     8821 ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa
     8881 cacacaagca gggagcagat actggcttaa ctatgcggca
//
