LOCUS       sequence-492536-        9292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAAV-RAM-ChR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K,
            Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, 
            Hemberg M, Yin JC, Maier SF, Lin Y
  TITLE     A robust activity marking system for exploring active neuronal 
            ensembles.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918.
  PUBMED    27661450
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e13918. doi"; pages: " 
            10.7554/eLife.13918"
COMMENT     Sequence Label: pAAV-RAM-d2TTA::TRE-ChR2-WPREpA
FEATURES             Location/Qualifiers
     source          1..9292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  894..1118
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1185..1718)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1888..1893,1894..1910,1911..1916))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   2256..2385
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        2405..2719
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2413..2431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2449..2467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2484..2502
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2520..2538
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2556..2574
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2591..2609
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2627..2645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2692..2716
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2737..3663
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      3673..3682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3679..3681,3682..3684,3685..4398)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3724..3745)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3985..4004)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4332..4353
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4445..5033
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4498..4518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4916..4927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5066..5083)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5072..5183
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    5331..5379
                     /note="synthetic polyadenylation signal"
     misc_feature    5393..5484
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5433..5452
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             join(5850..6467,6468..6473,6474..6509,6510..6512,6513..6548,
                     6549..6551,6552..6587,6588..6596)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6609..6625)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6650..6669)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6651..6669)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6695..6829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6858..6998
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6858..6987
                     /label=AAV2 ITR
     polyA_signal    7165..7299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  7328..7493
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7677..8131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7764..7783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7973..7994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8157..8261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8262..8330,8331..9122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8480..8499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa
      841 cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc
      901 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
      961 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
     1021 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
     1081 agaggggcgt ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg
     1141 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     1201 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     1261 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     1321 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     1381 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa
     1441 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     1501 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     1561 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     1621 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     1681 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     1741 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc
     1801 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     1861 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
     1921 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa
     1981 gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt
     2041 caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg
     2101 cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat
     2161 gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct
     2221 agctatagtt ctagtggttg gctacagctt gcatgcctgc aggcagctgc gcgctcgctc
     2281 gctcactgag gccgcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
     2341 gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc cgcacgcgcg
     2401 tctcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     2461 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     2521 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     2581 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     2641 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     2701 agtgaaccgt cagatcgcca ccggtgttta aacaccatgg actatggcgg cgctttgtct
     2761 gccgtcggac gcgaactttt gttcgttact aatcctgtgg tggtgaacgg gtccgtcctg
     2821 gtccctgagg atcaatgtta ctgtgccgga tggattgaat ctcgcggcac gaacggcgct
     2881 cagaccgcgt caaatgtcct gcagtggctt gcagcaggat tcagcatttt gctgctgatg
     2941 ttctatgcct accaaacctg gaaatctaca tgcggctggg aggagatcta tgtgtgcgcc
     3001 attgaaatgg ttaaggtgat tctcgagttc ttttttgagt ttaagaatcc ctctatgctc
     3061 taccttgcca caggacaccg ggtgcagtgg ctgcgctatg cagagtggct gctcacttgt
     3121 cctgtcatcc ttatccgcct gagcaacctc accggcctga gcaacgacta cagcaggaga
     3181 accatgggac tccttgtctc agacatcggg actatcgtgt ggggggctac cagcgccatg
     3241 gcaaccggct atgttaaagt catcttcttt tgtcttggat tgtgctatgg cgcgaacaca
     3301 ttttttcacg ccgccaaagc atatatcgag ggttatcata ctgtgccaaa gggtcggtgc
     3361 cgccaggtcg tgaccggcat ggcatggctg tttttcgtga gctggggtat gttcccaatt
     3421 ctcttcattt tggggcccga aggttttggc gtcctgagcg tctatggctc caccgtaggt
     3481 cacacgatta ttgatctgat gagtaaaaat tgttgggggt tgttgggaca ctacctgcgc
     3541 gtcctgatcc acgagcacat attgattcac ggagatatcc gcaaaaccac caaactgaac
     3601 atcggcggaa cggagatcga ggtcgagact ctcgtcgaag acgaagccga ggccggagcc
     3661 gtgccagcgg ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3781 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3841 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
     3901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3961 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4081 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4141 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4201 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4261 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     4321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4381 gacgagctgt acaagtaagg cgcgccagcg atccaccgga tctagataac tgatccttat
     4441 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4501 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4561 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4621 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4681 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4741 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4801 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4861 gctcgcctgt gttgccacct ggattctgcg cgggacgccc ttctgctacg tcccttcggc
     4921 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4981 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     5041 ccgtcgacta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5101 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5161 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5221 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcagact agtacgtctc
     5281 tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat
     5341 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
     5401 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
     5461 gtgcaggtgc cagaacattt ctctggccta actggccgac gcgtacccta gaagtttgtt
     5521 cgtgactcag actagaagtt tgttcgtgac tcagactaga agtttgttcg tgactcagac
     5581 tagaagtttg ttcgtgactc agaactcatt cataaaacgc ttgttataaa agcagtggct
     5641 gcggcgcctc gtactccaac cgcatctgca gcgagcaact gagaagccaa gactgagccg
     5701 gcggccggat ccagctcatg agccatggct tcccgccgga ggtggaggag caggatgatg
     5761 gcacgctgcc catgtcttgt gcccaggaga gcgggatgga ccgtcaccct gcagcctgtg
     5821 cttctgctag gatcaatgtg gaattcacca tgtctagact ggacaagagc aaagtcataa
     5881 actctgctct ggaattactc aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg
     5941 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     6001 tgctcgatgc cctggcaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     6061 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc
     6121 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     6181 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     6241 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gatcaggagc
     6301 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     6361 gacaagcaat tgagctgttc gaccatcagg gagccgaacc tgccttcctt ttcggcctgg
     6421 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg
     6481 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     6541 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa
     6601 gtaagcatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
     6661 gtcgtattac gcgcgcagcg gccgaccatg gcccaacttg tttattgcag cttataatgg
     6721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct ccggacacgt
     6841 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     6901 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     6961 ggcctcagtg agcgagcgag cgcgcagctg cctgcaggtc tgagacaata accctgataa
     7021 atgcttcaat aatgtaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     7081 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     7141 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     7201 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7261 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgat atcgcctagg
     7321 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     7381 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     7441 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     7501 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     7561 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
     7621 aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg aatgggacgc
     7681 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7741 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7801 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7861 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7921 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa tagtggactc
     7981 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8041 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8101 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     8161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8461 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8521 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8581 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8641 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8701 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8761 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8821 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8881 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8941 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9001 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9061 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9181 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
