LOCUS       sequence-49257-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Lum L, Yao S, Mozer B, Rovescalli A, Von Kessler D, Nirenberg M, 
            Beachy PA
  TITLE     Identification of Hedgehog pathway components by RNAi in Drosophila 
            cultured cells.
  JOURNAL   Science. 2003 Mar 28;299(5615):2039-45.
  PUBMED    12663920
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2003-03-28"; volume: "299(5615)"; pages: "2039-45"
COMMENT     Sequence Label: Copia Renilla Control
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(24..42)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(85..104)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          87..219
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 actttcgaag tcatggtggc tagcctatag tgagtcgtat taagtactct agccttaaga
       61 gctgtaattg aactgggagt ggacacctgt ggagagaaag gcaaagtgga tgtcagtaag
      121 accaataggt gcctatcaga aacgcaagag tcttctctgt ctcgacaagc ccagtttcta
      181 ttggtctcct taaacctgtc ttgtaacctt gatacttacc tgcccagtgc ctcacgacca
      241 acttctgcag tacccgggtg aattctacgc gtcgacaggc gccactagtg catgcatatg
      301 tgaagcttgg ctgcagattg tttagcttgt tcagctgcgc ttgtttattt gcttagcttt
      361 cgcttagcga cgtgttcact ttgcttgttt gaattgaatt gtcgctccgt agacgaagcg
      421 cctctattta tactccggcg ctcttttcgc gaacattcga ggcgcgctct ctcgaaccaa
      481 cgagagcagt atgccgttta ctgtgtgaca gagtgagaga gcattagtgc agagagggag
      541 agacccaaaa agaaaagaga gaataacgaa taacggccag agaaatttct cgagttttct
      601 ttctgccaaa caaatgacct accacaataa ccagtttgtt ttgggattct agaatattcg
      661 ctttattttg gaaatttctt tataaatacg gctgcttaaa taacttatgt taaagataat
      721 cgaagcgctt tgttatgcgt attccgccaa aaaaatactg cgcttctaaa ccaataaatg
      781 tctgatcggc atatgtatta aagattattt tgtactttta gttaatgaaa
//
