LOCUS       sequence-492666-       15807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting 
            Lctra, yellow, and RFPex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15807)
  TITLE     bioRxiv -- Conditional expression of Cas9 and dCas9 in Lucilia 
            cuprina reveals dCas9-associated lethality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: dCas9-ryt
FEATURES             Location/Qualifiers
     source          1..15807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    213..312
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     protein_bind    447..465
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    468..486
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    489..507
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    552..570
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    573..591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    594..612
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    615..633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    636..654
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    764..782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    814..832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1148..1195
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(1196..1219,1220..1222,1223..3778,3779..3781,
                     3782..5296)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5351..5371
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        complement(6397..6472)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(6571..6646)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(6745..6820)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10587..10686
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     repeat_region   12237..12299
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12356..12373)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12356..12372)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12365..12387)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12581..12600)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12700..12722
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12760..12778)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12846..12950
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12951..13019,13020..13811)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13169..13188)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13982..14570
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14471..14490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14724..14741
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14858..14879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14894..14899,14900..14917,14918..14924)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14932..14948
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14937..14959
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14956..14972
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14956..14972
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   15033..15067
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 aacacacaca cttataaaga tgagaacatt taacaaaaac aattataaaa ctgatggcaa
       61 tggagcaatg atgtgatgaa gctgatgaaa gttttgaata ttcattcaag agttaaaaac
      121 atgaacaaac atgcacaaaa ttagtcgaga ctgacggaca caccgaagcc ccggcggcaa
      181 ccctcagcgg atgccccggg gcttcacgtt ttcccaggtc agaagcggtt ttcgggagta
      241 gtgccccaac tggggtaacc tttgagttct ctcagttggg ggcgtagggt cgccgacatg
      301 acacaagggg ttgtgaccgg ggtggacacg tacgcgggtg cttacgaccg tcagtcgcgc
      361 gagcgcgact agtacaagcc gaattctgca gatggcgcgc caacctatgc ttatccgttt
      421 ttatttgtgt aatggccgca gaattctctc tatcactgat agggaagtct ctatcactga
      481 tagggacctc tctatcactg atagggaaat ctctatcact gatagggatc tctctatcac
      541 tgatagggac ttctctatca ctgataggga cgtctctatc actgataggg aactctctat
      601 cactgatagg gacatctcta tcactgatag ggacttctct atcactgata gggaagtatg
      661 ttctctctct tctcttctct ctctctttct cgaatgttct ctctcttctc ttctctctct
      721 ctttctcgat ggccggggcg cgccaggttt cgactttcac ttttctctat cactgatagg
      781 gagatccgag ctcgtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
      841 ctcgaagcgc cggatccaag gtttatgtga ttcatataaa tacggccgca caaactctct
      901 tcggcattag tacaatacaa acaatcaaag tgaaaacatt aaaaagaaat aacaagcgat
      961 ttaaattaag aaaaactacg ttcaagtgaa caagaaatta aataaatcag aaagtgtaca
     1021 aaagaaagta aaataattct ataaaaagtg aattttaagc aacaagttct ataaatagaa
     1081 aaactattac aaatcattca tcaagtttat atacacaaca aagaagaaaa acaaagtcac
     1141 acaaatgaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaaggacaa
     1201 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
     1261 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     1321 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     1381 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     1441 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     1501 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     1561 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     1621 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     1681 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     1741 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     1801 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     1861 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc
     1921 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     1981 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     2041 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     2101 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     2161 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     2221 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     2281 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     2341 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     2401 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     2461 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     2521 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     2581 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     2641 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     2701 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     2761 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     2821 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     2881 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     2941 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     3001 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     3061 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     3121 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     3181 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     3241 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     3301 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     3361 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     3421 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     3481 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     3541 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     3601 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     3661 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc acatcgtgcc
     3721 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaaggc
     3781 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     3841 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     3901 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     3961 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     4021 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     4081 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     4141 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     4201 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     4261 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     4321 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     4381 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     4441 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     4501 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     4561 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     4621 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     4681 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     4741 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     4801 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     4861 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     4921 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     4981 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     5041 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     5101 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     5161 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     5221 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     5281 tcagctggga ggcgacagcg ctggaggagg tggaagcgga ggaggaggaa gcggaggagg
     5341 aggtagcgga cctaagaaaa agaggaaggt gtaaatagaa tgaatcgttt ttaaaataac
     5401 aaatcaattg ttttataata ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt
     5461 aggaagatta cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac
     5521 gccacaattg tgtttgttgc aaataaaccc atgattattt gattaaaatt gttgttttct
     5581 ttgttcatag acaatagtgt gttttgccta aacgtgtact gcataaactc catgcgagtg
     5641 tatagcgagc tagtggctaa cgcttgcccc accaaagtag attcgtcaaa atcctcaatt
     5701 tcatcaccct cctccaagtt taacatttgg ccgtcggaat taacttctaa agatgccaca
     5761 taatctaata aatgaaatag agattcaaac gtggcgtcat cgtccgtttc gaccatttcc
     5821 gaaaagaact cgggcataaa ctctatgatt tctctggacg tggtgttgtc gaaactctca
     5881 aagtacgcag tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg aaacatgttg
     5941 ttgtaaccga acgggtccca tagcgccaaa accaaatctg ccagcgtcaa tagaatgagc
     6001 acgatgccga caatggagct ggcttggata gcgattcgag ttaactactg tccgttttta
     6061 tttgtgtaat aaaatgaacg gaacgctgaa ttctggaaca ttagaaattc tttgttatag
     6121 aatctataaa tttatgtcaa agttttgtcg tttcttatgc gatgagagat cttaaaaggc
     6181 aagtaaattg agctgtccta ttcaaacatt tttctattaa attgttggta ccttccgcgt
     6241 cgacgattca atatcgagca aaaatacgaa cgcatatgat tccatgaaac aaatgtattt
     6301 ctttagacgt ttctaaaacc aattccgaca attatttgat tctatgacaa ctccaaatag
     6361 ttttgtttat taaaaaataa aatttcaaca aaaaaagcac cgactcggtg ccactttttc
     6421 aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa acagatcttt
     6481 ccggtgtctt actgcaccag ccgggaatcg aacccgggtc tgtaccgtgg cagggtacta
     6541 ttctaccact agaccactgg tgctttgtta gcaccgactc ggtgccactt tttcaagttg
     6601 ataacggact agccttattt taacttgcta tttctagctc taaaacccaa tccttgcccc
     6661 tatgcttgca ccagccggga atcgaacccg ggtctgtacc gtggcagggt actattctac
     6721 cactagacca ctggtgcttt gttagcaccg actcggtgcc actttttcaa gttgataacg
     6781 gactagcctt attttaactt gctatttcta gctctaaaac tcgtagggtt taccttcacc
     6841 tgcatcggcc gggaatcgaa cccgggccgc ccgcgtggca ggcgagcatt ctaccactga
     6901 accaccgatg cttgatgtct acacacactc aaagcccaaa gatcatcact ttaataaact
     6961 atttaaccgt tctttttgag aaaaaagaat atgggaatta taaacaacaa tactatatgt
     7021 tgaaaacata aaaatatcat ttacagatta gggtttgtta ttatcacatt attactacct
     7081 gagtcgtcga cgccggatcc ttcaagttcc tcttgtaccc atcaaaatga ggccaaacta
     7141 gtgctgtttt taatgcactc actatgcatt tttatagctt cctctacacc tcggcaagtt
     7201 cattaaactt ttgccaagca ttttaacaat tactattttt agttgttttc cctctttttc
     7261 cccccattaa gtaataaaac attaaaaaat tttcttttgg aatattttca ttcaatgcag
     7321 cttaagcctt tcattttatt aggttcttgt ttttttcgtg tttttaaagt gcacttcttt
     7381 aaaatttgtt cttttcattc atgttttttc aaatttgaaa ataaaaaaaa tttacaagtg
     7441 ttttgttttg gattcgttat catttttaaa ataacagttt gtttaaaaaa aagtatattg
     7501 aatgtttagt tgagtagttt cagctaatag gtcgagaatt ttttcaatac acacttcttc
     7561 tgtctttttt ttggttttga tggttgtcga tgatgggttt ggctgcaagc ttttattgaa
     7621 acaagtggtg acgggcttcg gcacgttcgt attgttctac tacggtgtaa tcttcattat
     7681 gactggtaat atccaatttt gaatcgacgt agtagtaacc tggcaactta acgggctttt
     7741 tggccatgta gatggactta aattcaacca agtagtgacc accacccttc aatttcaaag
     7801 ccttatgaat ttcacctttc aagacaccat cacggggata caaacgttca gtagaaggtt
     7861 cccaacccaa ggttttcttt tgcataacaa gaccatctga tggaaagttt acaccgatga
     7921 atttgacgtg gtaaataaaa gtaccatctt gcaaagagga atcttgagta acggtgacaa
     7981 caccaccatc ttcgaaattc attacacgtt cccacttaaa accttcgggg aaggacaact
     8041 ttttataatc gggaatatca gcgggatgct taacgtatac cttagaaccg tattggaatt
     8101 gtggggacaa aatatcccag gcgaatggca agggaccacc cttggtaact tgcaatttag
     8161 cagtttgagt accttcgtag ggtttacctt caccttcacc ttcgatttca aattcgtgac
     8221 cattgacaga accttccatg tgtaccttaa aacgcataaa aggcttgata acattttcag
     8281 tagaatccat ggatccttta agctgagcaa tttcagcttg gaaagcaaaa gtttcaactt
     8341 cttcaggcat ctgtgtttaa taaaaaaaaa atagaaaaat acaaattatt agtaaaataa
     8401 tcaatcaaaa tatgaagtat aaataatttt aatgacgcac gtttttcgtt aagtgtaggg
     8461 agtgggattg gtactgaaaa tttttaataa aaatctagaa atctctttaa gttaagaatt
     8521 actagttttt taggggtgga ttgggtatgc tttttaacga atgaaaacat gaaatatcga
     8581 ttataatttc gaaaaaattt tgaaaaattt tctttaaaaa atcgcatgct agaaatttct
     8641 ttttattagc atggtgaaat tttctttata tactagattt attttctaaa aatgtcatgg
     8701 ccaaagaaaa tttaatctag aacaatttac aattacttag taacgttatt cttaaaccat
     8761 attgaaggtt tttctttctg gcaaattact tgagttttta catcacactt ttttcaaaag
     8821 aacaaaaacg ttttgttact acattttatt ttttttaata aaaacttacc ttgatgtgtt
     8881 tgttttatat taatttcaca aaaataaata ctttttaaat tatttttctt tttgcaattg
     8941 aaattaaaca actacacact tttttaatgt tcttttttca gacttttctt tattaagaaa
     9001 attaaaacac ttttaacttt ctcccgtttg gaattcttgg tgttttttgc acaatttttc
     9061 tttcaagact gatgccggtt agcagccatg cactgaattt ataggtaaaa caaaaataaa
     9121 cgaaaatact agaatgttct ggaacaaact acccgtactt ccgtaggctc taaacaacat
     9181 catgtggttg tttatttggt gataacaaaa gaaattggtt acattttaga tatataaaaa
     9241 aattaattta ttaaaattgt agaaaaccaa tattaattat aggtttttat tgttatttta
     9301 atttattagt tgcaaattat caaaaaaatg tgcaaaacat tcaacatgat taatgcccac
     9361 acagcgttgt atatatgtag aagcagtggc ttttaaactg taaaattgca tcaatgtcaa
     9421 aatttctaca atgttctctc tgtttttaat aacatgcgga atgaatcaac tataatttac
     9481 tttatttatt tatgcaaaaa tatataaaag ttattaatta aatgaaaaag gaattaatct
     9541 taggcattgc tgagtaatgt catgacataa actaaatatt tttctagtca aacaatgaac
     9601 ttatttattt caaaaacgtt ttattcttta aattatagtt aaaaagtctt attaattgac
     9661 aaatctatgt gtaattaagt gttttttgaa ctaagttaca agcttaatat gtatttgatt
     9721 aattgaaata tgtattaact attaattttt aaattaaata aatttaatat taattatata
     9781 ttaaactttg tgctttatat ggaaatagta tgtatgattt aatcacattc gatttacata
     9841 ttagtcatca taaattataa atgaaagtaa tttaattgtt ttattaaaga aaataataac
     9901 attattaaaa caataatatt gctatcaaaa tttataaaaa tgcataaaat gaaaaaatat
     9961 tgattttctc tcatcatggg ggcattaata attttttcgc cagtgcagct gatgtgcagt
    10021 aaaacatcag agttgccggt cttgaataaa aacaatgaaa agtggaataa tttaaaaatg
    10081 tgacaatcat atacttttaa catattttat aaaatttgac tgtaaaaatt taaaaattaa
    10141 aatttgcaaa cattatctca tacatattat aacaactctt ttgtttttat ataaatttaa
    10201 taaatgaaat caaaaattct ctcacaaatt tgtaatttcc aaaagtgtgg ccttttattt
    10261 tccccttttt aggtaaaaat ttcaaatatc tacaaccctg taaatgacag ctgtttgcgc
    10321 tcttttatgt attttttttg tttatcatgt cggtttttta gttcgcggtg caatttactc
    10381 gaaatttctt aaagtttgtt gaatttttgg cggtataatt ataatatttt gttttagtgt
    10441 tctttttatc aaattataat gtttaagtga attattttct gataatggtt tcaacacgtg
    10501 ggcccatctg cagaattcgg cttgtactag tcgcgctcgc gcgactgacg gtcgtaagca
    10561 cccgcgtacg tgtccacccc ggtcacaacc ccttgtgtca tgtcggcgac cctacgcccc
    10621 caactgagag aactcaaagg ttaccccagt tggggcacta ctcccgaaaa ccgcttctga
    10681 cctgggaaaa cgtgaagccc cggggcatcc gctgagggtt gccgccgggg cttcggtgtg
    10741 tccgtcagtg agctcctcag gtaataataa caactacaac caaaaaacaa aagcaacaac
    10801 aacgacaact tctacagggc aagtaacaaa acatagtatt tgtcaacgac acactatatg
    10861 aatgatgatg atgatgcaac aacagcaaca acagcaacaa cagctcgagt atcaacaaca
    10921 caacaaaaaa aactttttta aaaagacaca gctgctggca acaaaatacc acaaaagata
    10981 taaactaacg cagttgccac acacacacat acatacataa attaaaatat tatcaaacac
    11041 acacatatac atatatcaca acatacctat atacaaaacc acatttactt tgataaaagg
    11101 cgattcaaca aaaaagtagt cgtcatcctc cttcatttat ccttgcattt cacgtgtatc
    11161 tgtgtgtgtt ataaaacctg ctggccaaaa ggcaggtagg tagttgacaa cacaaatatt
    11221 ttacttcttt ttcatcatca gcatcaacaa cagcaggagc aacatattgt agacacacac
    11281 tctctgtttc ttcacttcac acacatacaa gtaaattatg acagtgaaaa agacgggtat
    11341 ttccaagctt cagtttcgtt tactttaggt cattaagatg actgtcctga tagcttgata
    11401 tcaatggaat caacgggatt gataaatgga attactgtga ttgataaata gcttcgaaag
    11461 acctcgttac ttcagggtac ttaacaaaat ttactgttgg ccgcgaaccg cgccatggcg
    11521 ccggccggga gccggcgcat gcgaaggtcg cctatggcat tattgtacgg aatgataaac
    11581 attgcctgca taaattcttt tattatatac agccataatg tcagtagcaa cggagaaaag
    11641 gttcaaagtc gcaaaaaatt tatgagaaac ctttacatga gcctgacgtc atcgtttatg
    11701 cgtaagcgtt tagaagctcc tactttgaag agatatttgc gcgataatat ctctaatatt
    11761 ttgccaaatg aagtgcctgg tacatcagat gacagtactg aagagccagt aatgaaaaaa
    11821 cgtacttact gtacttactg cccctctaaa ataaggcgaa aggcaaatgc atcgtgcaaa
    11881 aaatgcaaaa aagttatttg tcgagagcat aatattgata tgtgccaaag ttgtttctga
    11941 ctgactaata agtataattt gtttctatta tgtataagtt aagctaatta cttattttat
    12001 aatacaacat gactgttttt aaagtacaaa ataagtttat ttttgtaaaa gagagaatgt
    12061 ttaaaagttt tgttacttta tagaagaaat tttgagtttt tgtttttttt taataaataa
    12121 ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa
    12181 aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg
    12241 cgtcaatttt acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt
    12301 taagggcccg ggatccgata tctagatgca ttcgcgaggt accgagctcg aattcactgg
    12361 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    12421 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    12481 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
    12541 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
    12601 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    12661 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    12721 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
    12781 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
    12841 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    12901 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    12961 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    13021 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    13081 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    13141 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    13201 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    13261 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    13321 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    13381 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    13441 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    13501 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    13561 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    13621 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    13681 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    13741 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    13801 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    13861 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    13921 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    13981 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    14041 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    14101 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    14161 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    14221 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    14281 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    14341 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    14401 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    14461 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    14521 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    14581 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    14641 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    14701 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    14761 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    14821 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    14881 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    14941 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt gcatgcaggc
    15001 ctctgcagtc gacgggccca agcttccttt aaccctagaa agatagtctg cgtaaaattg
    15061 acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc
    15121 atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa
    15181 gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt
    15241 acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac
    15301 gtgataactt attatatata tattttcttg ttatagatat cgtgactaat atataataaa
    15361 atgggtagtt ctttagacga tgagcatatc ctctctgctc ttctgcaaag cgatgacgag
    15421 cttgttggtg aggattctga cagtgaaata tcagatcacg taagtgaaga tgacgtccag
    15481 agcgatacag aagaagcgtt tatagatgag gtacatgaag tgcagccaac gtcaagcggt
    15541 agtgaaatat tagacgaaca aaatgttatt gaacaaccag gttcttcatt ggcttctaac
    15601 agaatcttga ccttgcgaca gaggactatt agaggtaaga ataaacattg ttggtcaact
    15661 tcaaagtcca cgaggcgtag cggagtggta ccaggccctc gagtctgggc ccatgcatgg
    15721 gagctctccc aagatcttat ggtcgacctg caggcaacat tcatgtgtgg gtgattacac
    15781 tcacacaaaa acacacacat tcacaca
//
