LOCUS       sequence-492741-        8593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry plasmid (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Wang X, Clem J, Malzahn A, Qi Y
  TITLE     Increasing Cas12a- and Cas9-mediated deletion sizes with 
            exonucleases.
  JOURNAL   Plant Physiol. 2025 Oct 31;199(3):kiaf507. doi: 
            10.1093/plphys/kiaf507.
  PUBMED    41182408
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-10-31"; volume: "199(3):kiaf507. doi"; pages:
            " 10.1093/plphys/kiaf507"
COMMENT     Sequence Label: pYPQ166-TREX2-N
FEATURES             Location/Qualifiers
     source          1..8593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3295..3342
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3400..3465
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3472..3492
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3517..7617
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3517..7617
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7530..7538
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7618..7665
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    8340..8463
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8480..8499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8481..8499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8504..8520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8504..8520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggtacgttcc ctaccggccg
     2581 ccacatatgt cagaagcacc tcgtgccgag acctttgtct tcctggacct ggaagccact
     2641 gggctcccca gtgtggagcc cgagattgct gagctgtctc tatttgctgt ccaccgctcc
     2701 tccctggaga acccggagca cgacgagtct ggtgcactag tattgccacg ggtccttgac
     2761 aagcttacgc tgtgcatgtg ccctgagcgc cccttcactg ctaaggctag cgagatcacc
     2821 ggactgagca gtgaaggcct ggcgcgatgt cggaaggctg gctttgatgg cgcagtggtg
     2881 cggacactgc aggcattcct gagccgtcag gcagggccca tctgtcttgt ggcccacaat
     2941 ggatttgatt atgatttccc actgctgtgt gccgagctgc ggcgactggg tgctcgactg
     3001 ccccgggata ctgtctgcct ggacactctg ccggcactgc gtggcctgga ccgtgcccac
     3061 agtcacggca cccgggcacg gggtcgccag ggttacagtc tcggcagtct cttccatcgc
     3121 tacttccgag cagagccaag cgcagcccat tcagctgagg gcgacgtgca taccctgcta
     3181 ctgatcttcc tgcatcgcgc cgcagagcta ctcgcctggg cagatgagca agcccgtgga
     3241 tgggctcaca tcgagcccat gtacttaccg cctgatgatc ccagcctgga gtttaaacgt
     3301 cctgctgcca ccaaaaaggc cggacaggct aagaaaaaga aggagctctc aggatctgag
     3361 accccaggca caagtgaatc agctacacct gagtccatgg attacaagga ccacgacggg
     3421 gattacaagg accacgacat tgattacaag gatgatgatg acaagatggc tccgaagaag
     3481 aagaggaagg ttggcatcca cggggtgcca gctgctgaca agaagtactc gatcggcctc
     3541 gatattggga ctaactctgt tggctgggcc gtgatcaccg acgagtacaa ggtgccctca
     3601 aagaagttca aggtcctggg caacaccgat cggcattcca tcaagaagaa tctcattggc
     3661 gctctcctgt tcgacagcgg cgagacggct gaggctacgc ggctcaagcg caccgcccgc
     3721 aggcggtaca cgcgcaggaa gaatcgcatc tgctacctgc aggagatttt ctccaacgag
     3781 atggcgaagg ttgacgattc tttcttccac aggctggagg agtcattcct cgtggaggag
     3841 gataagaagc acgagcggca tccaatcttc ggcaacattg tcgacgaggt tgcctaccac
     3901 gagaagtacc ctacgatcta ccatctgcgg aagaagctcg tggactccac agataaggcg
     3961 gacctccgcc tgatctacct cgctctggcc cacatgatta agttcagggg ccatttcctg
     4021 atcgaggggg atctcaaccc ggacaatagc gatgttgaca agctgttcat ccagctcgtg
     4081 cagacgtaca accagctctt cgaggagaac cccattaatg cgtcaggcgt cgacgcgaag
     4141 gctatcctgt ccgctaggct ctcgaagtct cggcgcctcg agaacctgat cgcccagctg
     4201 ccgggcgaga agaagaacgg cctgttcggg aatctcattg cgctcagcct ggggctcacg
     4261 cccaacttca agtcgaattt cgatctcgct gaggacgcca agctgcagct ctccaaggac
     4321 acatacgacg atgacctgga taacctcctg gcccagatcg gcgatcagta cgcggacctg
     4381 ttcctcgctg ccaagaatct gtcggacgcc atcctcctgt ctgatattct cagggtgaac
     4441 accgagatta cgaaggctcc gctctcagcc tccatgatca agcgctacga cgagcaccat
     4501 caggatctga ccctcctgaa ggcgctggtc aggcagcagc tccccgagaa gtacaaggag
     4561 atcttcttcg atcagtcgaa gaacggctac gctgggtaca ttgacggcgg ggcctctcag
     4621 gaggagttct acaagttcat caagccgatt ctggagaaga tggacggcac ggaggagctg
     4681 ctggtgaagc tcaatcgcga ggacctcctg aggaagcagc ggacattcga taacggcagc
     4741 atcccacacc agattcatct cggggagctg cacgctatcc tgaggaggca ggaggacttc
     4801 taccctttcc tcaaggataa ccgcgagaag atcgagaaga ttctgacttt caggatcccg
     4861 tactacgtcg gcccactcgc taggggcaac tcccgcttcg cttggatgac ccgcaagtca
     4921 gaggagacga tcacgccgtg gaacttcgag gaggtggtcg acaagggcgc tagcgctcag
     4981 tcgttcatcg agaggatgac gaatttcgac aagaacctgc caaatgagaa ggtgctccct
     5041 aagcactcgc tcctgtacga gtacttcaca gtctacaacg agctgactaa ggtgaagtat
     5101 gtgaccgagg gcatgaggaa gccggctttc ctgtctgggg agcagaagaa ggccatcgtg
     5161 gacctcctgt tcaagaccaa ccggaaggtc acggttaagc agctcaagga ggactacttc
     5221 aagaagattg agtgcttcga ttcggtcgag atctctggcg ttgaggaccg cttcaacgcc
     5281 tccctgggga cctaccacga tctcctgaag atcattaagg ataaggactt cctggacaac
     5341 gaggagaatg aggatatcct cgaggacatt gtgctgacac tcactctgtt cgaggaccgg
     5401 gagatgatcg aggagcgcct gaagacttac gcccatctct tcgatgacaa ggtcatgaag
     5461 cagctcaaga ggaggaggta caccggctgg gggaggctga gcaggaagct catcaacggc
     5521 attcgggaca agcagtccgg gaagacgatc ctcgacttcc tgaagagcga tggcttcgcg
     5581 aaccgcaatt tcatgcagct gattcacgat gacagcctca cattcaagga ggatatccag
     5641 aaggctcagg tgagcggcca gggggactcg ctgcacgagc atatcgcgaa cctcgctggc
     5701 tcgccagcta tcaagaaggg gattctgcag accgtgaagg ttgtggacga gctggtgaag
     5761 gtcatgggca ggcacaagcc tgagaacatc gtcattgaga tggcccggga gaatcagacc
     5821 acgcagaagg gccagaagaa ctcacgcgag aggatgaaga ggatcgagga gggcattaag
     5881 gagctggggt cccagatcct caaggagcac ccggtggaga acacgcagct gcagaatgag
     5941 aagctctacc tgtactacct ccagaatggc cgcgatatgt atgtggacca ggagctggat
     6001 attaacaggc tcagcgatta cgacgtcgat catatcgttc cacagtcatt cctgaaggat
     6061 gactccattg acaacaaggt cctcaccagg tcggacaaga accggggcaa gtctgataat
     6121 gttccttcag aggaggtcgt taagaagatg aagaactact ggcgccagct cctgaatgcc
     6181 aagctgatca cgcagcggaa gttcgataac ctcacaaagg ctgagagggg cgggctctct
     6241 gagctggaca aggcgggctt catcaagagg cagctggtcg agacacggca gatcactaag
     6301 cacgttgcgc agattctcga ctcacggatg aacactaagt acgatgagaa tgacaagctg
     6361 atccgcgagg tgaaggtcat caccctgaag tcaaagctcg tctccgactt caggaaggat
     6421 ttccagttct acaaggttcg ggagatcaac aattaccacc atgcccatga cgcgtacctg
     6481 aacgcggtgg tcggcacagc tctgatcaag aagtacccaa agctcgagag cgagttcgtg
     6541 tacggggact acaaggttta cgatgtgagg aagatgatcg ccaagtcgga gcaggagatt
     6601 ggcaaggcta ccgccaagta cttcttctac tctaacatta tgaatttctt caagacagag
     6661 atcactctgg ccaatggcga gatccggaag cgccccctca tcgagacgaa cggcgagacg
     6721 ggggagatcg tgtgggacaa gggcagggat ttcgcgaccg tcaggaaggt tctctccatg
     6781 ccacaagtga atatcgtcaa gaagacagag gtccagactg gcgggttctc taaggagtca
     6841 attctgccta agcggaacag cgacaagctc atcgcccgca agaaggactg ggatccgaag
     6901 aagtacggcg ggttcgacag ccccactgtg gcctactcgg tcctggttgt ggcgaaggtt
     6961 gagaagggca agtccaagaa gctcaagagc gtgaaggagc tgctggggat cacgattatg
     7021 gagcgctcca gcttcgagaa gaacccgatc gatttcctgg aggcgaaggg ctacaaggag
     7081 gtgaagaagg acctgatcat taagctcccc aagtactcac tcttcgagct ggagaacggc
     7141 aggaagcgga tgctggcttc cgctggcgag ctgcagaagg ggaacgagct ggctctgccg
     7201 tccaagtatg tgaacttcct ctacctggcc tcccactacg agaagctcaa gggcagcccc
     7261 gaggacaacg agcagaagca gctgttcgtc gagcagcaca agcattacct cgacgagatc
     7321 attgagcaga tttccgagtt ctccaagcgc gtgatcctgg ccgacgcgaa tctggataag
     7381 gtcctctccg cgtacaacaa gcaccgcgac aagccaatca gggagcaggc tgagaatatc
     7441 attcatctct tcaccctgac gaacctcggc gcccctgctg ctttcaagta cttcgacaca
     7501 actatcgatc gcaagaggta cacaagcact aaggaggtcc tggacgcgac cctcatccac
     7561 cagtcgatta ccggcctcta cgagacgcgc atcgacctgt ctcagctcgg gggcgacaag
     7621 cggccagcgg cgacgaagaa ggcggggcag gcgaagaaga agaagtgagc tcagagcttt
     7681 cgttcgtatc atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca
     7741 cacaccagaa tcctactgag tttgagtatt atggcattgg gaaaactgtt tttcttgtac
     7801 catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa
     7861 atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta
     7921 atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc
     7981 aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata
     8041 tgaggagtaa aacacttgta gttgtaccat tatgcttatt cactaggcaa caaatatatt
     8101 ttcagaccta gaaaagctgc aaatgttact gaatacaagt atgtcctctt gtgttttaga
     8161 catttatgaa ctttccttta tgtaattttc cagaatcctt gtcagattct aatcattgct
     8221 ttataattat agttatactc atggatttgt agttgagtat gaaaatattt tttaatgcat
     8281 tttatgactt gccaattgtt ccggaactct agataagctt accggaaagg gcgaattcgc
     8341 aactttgtat acaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     8401 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     8461 atgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     8521 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     8581 atcatgcctc ctc
//
