LOCUS       sequence-49275-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Vorvis C, Markus SM, Lee WL
  TITLE     Photoactivatable GFP tagging cassettes for protein-tracking studies 
            in the budding yeast Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2008 Sep;25(9):651-9.
  PUBMED    18727145
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2008-09"; volume: "25(9)"; pages: "651-9"
COMMENT     Sequence Label: tandem PA-GFP-KanR
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..393)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(484..505)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     833..852
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctgtggat taatcgcggc ctcgaaacgt gagtcttttc cttacccatg gttgtttatg
       61 ttcggatgtg atgtgagaac tgtatcctag caagatttta aaaggaagta tatgaaagaa
      121 gaacctcagt ggcaaatcct aaccttttat atttctctac aggggcgcgg cgtggggaca
      181 attcaacgcg tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta
      241 atttttgctt cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg
      301 atgtatgggc taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag
      361 actgtcaagg agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga
      421 ggcaagctaa acagatctct acttgtacag ctcgtccatg ccgagagtga tcccggcggc
      481 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      541 ggactggtgg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      601 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      661 gaagttggcc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
      721 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      781 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      841 gttgccgtcg tccttgaaga agatggtgcg ctcct
//
