LOCUS       sequence-49277-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Markus SM, Punch JJ, Lee WL
  TITLE     Motor- and tail-dependent targeting of dynein to microtubule plus 
            ends and the cell cortex.
  JOURNAL   Curr Biol. 2009 Feb 10;19(3):196-205. Epub 2009 Jan 29.
  PUBMED    19185494
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2009-02-10"; volume: "19(3)"; pages: "196-205. Epub 
            2009 Jan 29"
COMMENT     Sequence Label: pRS306:3mCherry
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..770
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(205..223)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     473..492
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(772..788)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(772..788)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagact aggagatcca
       61 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      121 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      181 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
      241 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
      301 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      361 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      421 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
      481 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
      541 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
      601 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
      661 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
      721 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag agatccacta
      781 gttctagagc ggccgccacc gcggtggagc tccaattcgc cctatagtga gtc
//
