LOCUS       sequence-49281-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Chung HS, Raetz CR
  TITLE     Interchangeable domains in the Kdo transferases of Escherichia coli 
            and Haemophilus influenzae.
  JOURNAL   Biochemistry. 2010 May 18;49(19):4126-37.
  PUBMED    20394418
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2010-05-18"; volume: "49(19)"; pages: 
            "4126-37"
COMMENT     Sequence Label: pBAD33.1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             74..96
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             104..136
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(202..219)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      372..458
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      550..577
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        596..687
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tactgtttct ccatacccgt ttttttgggc tagcgaattc gagctcggta cccggggatc
       61 ctctagaaat aattttgttt aactttaaga aggagatata catatggcta gcatgactgg
      121 tggacagcaa atgggtcggg atccgaattc gagctccgtc gacaagcttg gctgttttgg
      181 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
      241 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
      301 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
      361 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      421 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
      481 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
      541 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
      601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      781 aaagatgctg aagatcagtt gggtgcag
//
