LOCUS       sequence-49286-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Markus SM, Punch JJ, Lee WL
  TITLE     Motor- and tail-dependent targeting of dynein to microtubule plus 
            ends and the cell cortex.
  JOURNAL   Curr Biol. 2009 Feb 10;19(3):196-205. Epub 2009 Jan 29.
  PUBMED    19185494
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2009-02-10"; volume: "19(3)"; pages: "196-205. Epub 
            2009 Jan 29"
COMMENT     Sequence Label: pRS303:3mCherry
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             184..894
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(329..347)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     597..616
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(896..912)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(896..912)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
       61 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      121 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gactaggaga
      181 tccatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      241 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      301 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      361 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      421 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      481 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      541 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      601 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      661 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      721 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      781 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      841 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtagagatcc
      901 actagttcta gagcggccgc caccgcggtg gagctccaat tcgccctata gt
//
