LOCUS       sequence-492883-        8507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SpCas9 and a sgRNA targeting the human UBF loci for 
            knock-in..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Ide S, Imai R, Ochi H, Maeshima K
  TITLE     Transcriptional suppression of ribosomal DNA with phase separation.
  JOURNAL   Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 
            10.1126/sciadv.abb5953. Print 2020 Oct.
  PUBMED    33055158
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-10-14"; volume: "6(42). pii: 6/42/eabb5953. doi"; pages:
            " 10.1126/sciadv.abb5953. Print 2020 Oct"
COMMENT     Sequence Label: pX330-UBF
FEATURES             Location/Qualifiers
     source          1..8507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     165..186
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(340..359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     459..481
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(519..537)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        605..709
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(710..778,779..1570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(928..947)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1741..2329
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2230..2249
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2391..2639
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2391..2411
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2562..2581
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2661..2736
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        2833..3118
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        3120..3396
                     /label=chicken beta-actin promoter
     intron          3397..3624
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      3636..3645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3645..3710
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3717..3737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3762..7862
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3762..7862
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7863..7910
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(7938..7955)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7944..8151
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8160..8300
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8160..8289
                     /label=AAV2 ITR
     rep_origin      join(8375..8507,1..323)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8462..8481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
       61 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
      121 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      181 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
      241 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
      301 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
      361 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      421 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      481 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
      541 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      601 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      661 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      721 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      781 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      841 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      901 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      961 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1021 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1081 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1141 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1201 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1261 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1321 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1381 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat
     1441 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1501 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1561 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1621 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1681 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1741 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1801 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1861 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1921 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1981 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2041 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2101 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2161 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2221 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2281 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2341 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
     2401 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     2461 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     2521 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     2581 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
     2641 ttccaggtct gtggggcagt gttttagagc tagaaatagc aagttaaaat aaggctagtc
     2701 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata
     2761 gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc
     2821 tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2881 cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac gtcaatgggt
     2941 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3001 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc agtacatgac
     3061 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     3121 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     3181 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     3241 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
     3301 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
     3361 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
     3421 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     3481 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag
     3541 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac
     3601 ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catggactat aaggaccacg
     3661 acggagacta caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa
     3721 agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg
     3781 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
     3841 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
     3901 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
     3961 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
     4021 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
     4081 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     4141 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     4201 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     4261 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     4321 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     4381 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
     4441 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
     4501 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     4561 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     4621 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     4681 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     4741 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     4801 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     4861 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     4921 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     4981 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     5041 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     5101 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     5161 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     5221 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     5281 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     5341 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     5401 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     5461 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     5521 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     5581 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     5641 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     5701 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     5761 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     5821 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     5881 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     5941 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     6001 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     6061 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     6121 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     6181 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     6241 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga
     6301 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     6361 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     6421 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     6481 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     6541 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     6601 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     6661 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     6721 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     6781 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     6841 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     6901 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     6961 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     7021 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     7081 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     7141 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     7201 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     7261 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     7321 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     7381 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     7441 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     7501 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     7561 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     7621 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     7681 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     7741 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     7801 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
     7861 acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag taagaattcc
     7921 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7981 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     8041 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     8101 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca
     8161 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     8221 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     8281 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     8341 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     8401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     8461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctt
//
